Functional analysis of serially expanded human iPS cell-derived RPE cultures

Investigative Ophthalmology and Visual Science

Volumn 54, Issue 10, 2013, Pages 6767-6778

  Singh, Ruchira ^a   Phillips, M Joseph ^a,b   Kuai, David ^a   Meyer, Jackelyn ^a   Martin, Jessica M ^a   Smith, Molly A ^a   Perez, Enio T ^a   Shen, Wei ^a   Wallace, Kyle A ^a   Capowski, Elizabeth E ^a   Wright, Lynda S ^a   Gamm, David M ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

Induced pluripotent stem cell; Passaging; Retinal pigment epithelium

Indexed keywords

ADENOSINE TRIPHOSPHATE; CALCIUM; G PROTEIN COUPLED RECEPTOR; PIGMENT EPITHELIUM DERIVED FACTOR; VASCULOTROPIN;

ARTICLE; CELL PHAGOCYTOSIS; CONTROLLED STUDY; FIBROBLAST; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENETIC VARIABILITY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; PHOTORECEPTOR; PIGMENT EPITHELIUM; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RETINA TEAR; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKIN BIOPSY; STEM CELL EXPANSION; WESTERN BLOTTING;

INDUCED PLURIPOTENT STEM CELL; PASSAGING; RETINAL PIGMENT EPITHELIUM;

ANIMALS; BLOTTING, WESTERN; CATTLE; CELL DIFFERENTIATION; CELL LINE; EMBRYONIC STEM CELLS; EYE PROTEINS; FIBROBLASTS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HUMANS; IMMUNOHISTOCHEMISTRY; PHAGOCYTOSIS; REAL-TIME POLYMERASE CHAIN REACTION; RETINAL PIGMENT EPITHELIUM; RNA;

EID: 84886877711     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.13-11943     Document Type: Article

References (38)

1. Lim LS, Mitchell P, Seddon JM, Holz FG, Wong TY. Age-related macular degeneration. Lancet. 2012; 379: 1728-1738.
2. Strauss O. The retinal pigment epithelium in visual function. Physiol Rev. 2005; 85: 845-881.
3. Meyer JS, Howden SE, Wallace KA, et al. Optic vesicle-like structures derived from human pluripotent stem cells facilitate a customized approach to retinal disease treatment. Stem Cells. 2011; 29: 1206-1218.
4. Howden SE, Gore A, Li Z, et al. Genetic correction and analysis of induced pluripotent stem cells from a patient with gyrate atrophy. Proc Natl Acad Sci U S A. 2011; 108: 6537-6542.
5. Singh R, Shen W, Kuai D, et al. iPS cell modeling of Best disease: insights into the pathophysiology of an inherited macular degeneration. Hum Mol Genet. 2012; 22: 593-607.
6. Du H, Lim SL, Grob S, Zhang K. Induced pluripotent stem cell therapies for geographic atrophy of age-related macular degeneration. Semin Ophthalmol. 2011; 26: 216-224.
7. Ukrohne TU, Westenskow PD, Kurihara T, et al. Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells. Stem Cells Transl Med. 2012; 1: 96-109.
8. Hirami Y, Osakada F, Takahashi K, et al. Generation of retinal cells from mouse and human induced pluripotent stem cells. Neurosci Lett. 2009; 458: 126-131.
9. Buchholz DE, Hikita ST, Rowland TJ, et al. Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells. Stem Cells. 2009; 27: 2427-2434.
10. Rowland TJ, Buchholz DE, Clegg DO. Pluripotent human stem cells for the treatment of retinal disease. J Cell Physiol. 2012; 227: 457-466.
11. Meyer JS, Shearer RL, Capowski EE, et al. Modeling early retinal development with human embryonic and induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2009; 106: 16698-16703.
12. Bharti K, Miller SS, Arnheiter H. The new paradigm: retinal pigment epithelium cells generated from embryonic or induced pluripotent stem cells. Pigment Cell Melanoma Res. 2011; 24: 21-34.
13. Kokkinaki M, Sahibzada N, Golestaneh N. Human induced pluripotent stem-derived retinal pigment epithelium (RPE) cells exhibit ion transport, membrane potential, polarized vascular endothelial growth factor secretion, and gene expression pattern similar to native RPE. Stem Cells. 2011; 29: 825-835.
14. Carr AJ, Vugler AA, Hikita ST, et al. Protective effects of human iPS-derived retinal pigment epithelium cell transplantation in the retinal dystrophic rat. PLoS One. 2009; 4: e8152.
15. Phillips MJ, Wallace KA, Dickerson SJ, et al. Blood-derived human iPS cells generate optic vesicle-like structures with the capacity to form retinal laminae and develop synapses. Invest Ophthalmol Vis Sci. 2012; 53: 2007-2019.
16. Gamm DM, Melvan JN, Shearer RL, et al. A novel serum-free method for culturing human prenatal retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 2008; 49: 788-799.
17. Sonoda S, Spee C, Barron E, Ryan SJ, Kannan R, Hinton DR. A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells. Nat Protoc. 2009; 4: 662-673.
18. Mitchell CH. Release of ATP by a human retinal pigment epithelial cell line: potential for autocrine stimulation through subretinal space. J Physiol. 2001; 534: 193-202.
19. Blaauwgeers HG, Holtkamp GM, Rutten H, et al. Polarized vascular endothelial growth factor secretion by human retinal pigment epithelium and localization of vascular endothelial growth factor receptors on the inner choriocapillaris. Evidence for a trophic paracrine relation. Am J Pathol. 1999; 155: 421-428.
20. Maminishkis A, Chen S, Jalickee S, et al. Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue. Invest Ophthalmol Vis Sci. 2006; 47: 3612-3624.
21. Vaajasaari H, Ilmarinen T, Juuti-Uusitalo K, et al. Toward the defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cells. Mol Vis. 2011; 17: 558-575.
22. Smith-Thomas L, Haycock JW, Metcalfe R, et al. Involvement of calcium in retinal pigment epithelial cell proliferation and pigmentation. Curr Eye Res. 1998; 17: 813-822.
23. Wimmers S, Karl MO, Strauss O. Ion channels in the RPE. Prog Retin Eye Res. 2007; 26: 263-301.
24. Rosenthal R, Heimann H, Agostini H, Martin G, Hansen LL, Strauss O. Ca2+channels in retinal pigment epithelial cells regulate vascular endothelial growth factor secretion rates in health and disease. Mol Vis. 2007; 13: 443-456.
25. Karl MO, Kroeger W, Wimmers S, et al. Endogenous Gas6 and Ca2+-channel activation modulate phagocytosis by retinal pigment epithelium. Cell Signal. 2008; 20: 1159-1168.
26. Hall MO, Abrams TA, Mittag TW. ROS ingestion by RPE cells is turned off by increased protein kinase C activity and by increased calcium. Exp Eye Res. 1991; 52: 591-598.
27. Feldman EL, Randolph AE. Peptides stimulate phosphoinositide hydrolysis in human retinal pigment epithelium. Invest Ophthalmol Vis Sci. 1993; 34: 431-437.
28. Peterson WM, Meggyesy C, Yu K, Miller SS. Extracellular ATP activates calcium signaling, ion, and fluid transport in retinal pigment epithelium. J Neurosci. 1997; 17: 2324-2337.
29. Grisanti S, Guidry C. Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype. Invest Ophthalmol Vis Sci. 1995; 36: 391-405.
30. Lee SC, Kwon OW, Seong GJ, Kim SH, Ahn JE, Kay ED. Epitheliomesenchymal transdifferentiation of cultured RPE cells. Ophthalmic Res. 2001; 33: 80-86.
31. Shi JW, Liu W, Zhang TT, et al. The enforced expression of c-Myc in pig fibroblasts triggers mesenchymal-epithelial transition (MET) via F-actin reorganization and RhoA/Rock pathway inactivation. Cell Cycle. 2013; 12: 1119-1127.
32. Fuchs U, Kivela T, Tarkkanen A. Cytoskeleton in normal and reactive human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 1991; 32: 3178-3186.
33. Grossniklaus HE, Ling JX, Wallace TM, et al. Macrophage and retinal pigment epithelium expression of angiogenic cytokines in choroidal neovascularization. Mol Vis. 2002; 8: 119-126.
34. Sonoda S, Sreekumar PG, Kase S, et al. Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: relevance to age-related macular degeneration. Aging (Albany NY). 2009; 2: 28-42.
35. Westenskow PD, Moreno SK, Krohne TU, et al. Using flow cytometry to compare the dynamics of photoreceptor outer segment phagocytosis in iPS-derived RPE cells. Invest Ophthalmol Vis Sci. 2012; 53: 6282-6290.
36. Tezel TH, Del Priore LV. Serum-free media for culturing and serial-passaging of adult human retinal pigment epithelium. Exp Eye Res. 1998; 66: 807-815.
37. Yamanaka S. A fresh look at iPS cells. Cell. 2009; 137: 13-17.
38. Toivonen S, Ojala M, Hyysalo A, et al. Comparative analysis of targeted differentiation of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells reveals variability associated with incomplete transgene silencing in retrovirally derived hiPSC lines. Stem Cells Transl Med. 2013; 2: 83-93.