Construction of a random circular permutation library using an engineered transposon

Analytical Biochemistry

Volumn 474, Issue , 2015, Pages 16-24

  Pierre, Brennal ^a   Shah, Vandan ^a   Xiao, Jenny ^a   Kim, Jin Ryoun ^a  

^a JACOBS ENGINEERING   (United States)

Author keywords

Circular permutation; Combinatorial library; Protein engineering; Transposon

Indexed keywords

BIOCHEMICAL ENGINEERING; GENETIC ENGINEERING; LIBRARIES;

CIRCULAR PERMUTATION; COMBINATORIAL LIBRARY; COMPREHENSIVE EVALUATION; CONVENTIONAL METHODS; PROTEIN ENGINEERING; SEQUENCE MODIFICATION; THREE-DIMENSIONAL STRUCTURE; TRANSPOSON;

PROTEINS;

AMINO ACID SEQUENCE; ARTICLE; BIOENGINEERING; CIRCULAR PERMUTATION; CLONING VECTOR; COMBINATORIAL LIBRARY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN STRUCTURE; TRANSPOSON; AGAR GEL ELECTROPHORESIS; GENE EXPRESSION REGULATION; GENE LIBRARY; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METABOLISM; MOLECULAR GENETICS; PLASMID; PROCEDURES;

CIRCULAR DNA; TRANSPOSON;

BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; DNA, CIRCULAR; ELECTROPHORESIS, AGAR GEL; GENE LIBRARY; GENETIC ENGINEERING; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PLASMIDS;

EID: 84923351044     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.12.011     Document Type: Article

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