Enhanced catalytic efficiency of aminoglycoside phosphotransferase (3′)-IIa achieved through protein fragmentation and reassembly

Journal of Molecular Biology

Volumn 353, Issue 1, 2005, Pages 26-37

  Paschon, David E ^a   Patel, Zarana S ^a   Ostermeier, Marc ^a  

^a Johns Hopkins University   (United States)

Author keywords

Aminoglycoside phosphotransferase; Protein complementation assay; Protein engineering; Protein fragment complementation

Indexed keywords

AMINOGLYCOSIDE; KANAMYCIN; PHOSPHOTRANSFERASE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; DIMERIZATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE FUSION; GENE LIBRARY; GENE LOCATION; GENE MUTATION; KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN FUNCTION; SEQUENCE HOMOLOGY; WILD TYPE;

ESCHERICHIA COLI;

EID: 25144503362     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2005.08.026     Document Type: Article

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