Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing

American Journal of Physiology - Renal Physiology

Volumn 308, Issue 4, 2015, Pages F366-F376

  Roy, Ankita ^a   Goodman, Joshua H ^a   Begum, Gulnaz ^a   Donnelly, Bridget F ^a   Pittman, Gabrielle ^a   Weinman, Edward J ^b   Sun, Dandan ^a,c   Subramanya, Arohan R ^a,c  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

CRISPR Cas system; Genome editing; SLC12 cotransporters; WNK1

Indexed keywords

CRISPR ASSOCIATED PROTEIN; PROTEIN KINASE WNK1; SODIUM CHLORIDE COTRANSPORTER; UBIQUITIN PROTEIN LIGASE E3; CARRIER PROTEIN; CUL3 PROTEIN, HUMAN; CULLIN; KLHL3 PROTEIN, HUMAN; OSR1 PROTEIN, HUMAN; PROTEIN SERINE THREONINE KINASE; SIGNAL PEPTIDE; STK39 PROTEIN, HUMAN; TRANSCRIPTION FACTOR; WNK1 PROTEIN, HUMAN; WNK4 PROTEIN, HUMAN;

ARTICLE; CELL LINE; CELL VOLUME; CONTROLLED STUDY; GENOME; HUMAN; HUMAN CELL; HYPEROSMOTIC STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STABILITY; CELL SHAPE; CELL SIZE; CRISPR CAS SYSTEM; DEFICIENCY; DOWN REGULATION; EXON; GENE SILENCING; GENETIC TRANSFECTION; GENETICS; GENOTYPE; HEK293 CELL LINE; HUMAN GENOME; METABOLISM; OSMOTIC PRESSURE; PHENOTYPE; PROCEDURES; SIGNAL TRANSDUCTION; TIME FACTOR;

CARRIER PROTEINS; CELL SHAPE; CELL SIZE; CRISPR-CAS SYSTEMS; CULLIN PROTEINS; DOWN-REGULATION; EXONS; GENE KNOCKDOWN TECHNIQUES; GENOME, HUMAN; GENOTYPE; HEK293 CELLS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; OSMOTIC PRESSURE; PHENOTYPE; PROTEIN STABILITY; PROTEIN-SERINE-THREONINE KINASES; SIGNAL TRANSDUCTION; TIME FACTORS; TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 84922894848     PISSN: 1931857X     EISSN: 15221466     Source Type: Journal    
DOI: 10.1152/ajprenal.00612.2014     Document Type: Article

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