Remediating agitation-induced antibody Aggregation by eradicating exposed hydrophobic motifs

mAbs

Volumn 6, Issue 6, 2014, Pages 1540-1550

  Clark, Rutilio H ^a   Latypov, Ramil F ^a   De Imus, Cyr ^a   Carter, Jane ^a   Wilson, Zien ^a   Manchulenko, Kathy ^a   Brown, Michael E ^a   Ketchem, Randal R ^a  

^a AMGEN INC   (United States)

Author keywords

Aggregation; Agitation; Antibody; Computational; Engineering; In silico; Modeling; Stability; Thermal

Indexed keywords

IMMUNOGLOBULIN G2; GAMMA INTERFERON; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; PROTEIN AGGREGATE; PROTEIN BINDING;

AGITATION INDUCED ANTIBODY AGGREGATION; ALGORITHM; ANTIBODY COMBINING SITE; ANTIBODY ENGINEERING; ANTIBODY TITER; ARTICLE; BIOLOGICAL ACTIVITY; HYDROPHOBICITY; PROTEIN AGGREGATION; PROTEIN MOTIF; SPATIAL AGGREGATION PROPENSITY; TEMPERATURE STRESS; THERMOSTABILITY; ANTIBODY AFFINITY; BIOLOGY; CELL CULTURE; CHEMICAL PHENOMENA; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECTS; HEK293 CELL LINE; HUMAN; IMMUNOLOGY; MECHANICAL STRESS; METABOLISM; NATURAL KILLER CELL; PROCEDURES; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE;

ALGORITHMS; AMINO ACID MOTIFS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; CELLS, CULTURED; COMPUTATIONAL BIOLOGY; HEK293 CELLS; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; IMMUNOGLOBULIN G; INTERFERON-GAMMA; KILLER CELLS, NATURAL; MODELS, MOLECULAR; PROTEIN AGGREGATES; PROTEIN BINDING; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; STRESS, MECHANICAL;

EID: 84920845095     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.4161/mabs.36252     Document Type: Article

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