Relationship between thermal stability, degradation rate and expression yield of barnase variants in the periplasm of Escherichia coli

Protein Engineering

Volumn 9, Issue 12, 1996, Pages 1197-1202

  Kwon, Won Shik ^a   Da Silva, Nancy A ^a   Kellis Jr , James T ^b  

^a University of California   (United States)

^b GENENCOR INTERNATIONAL INC   (United States)

Author keywords

Barnase; Periplasmic degradation; Thermal stability

Indexed keywords

BACILLUS AMYLOLIQUEFACIENS; ESCHERICHIA COLI;

ALKALINE PHOSPHATASE; BACTERIAL ENZYME; BARNASE; MUTANT PROTEIN; RECOMBINANT PROTEIN; RIBONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; EXTRACELLULAR FLUID; GENETIC ENGINEERING; HALF LIFE TIME; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN STABILITY; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; THERMOSTABILITY;

BACILLUS; ENZYME STABILITY; ESCHERICHIA COLI; HALF-LIFE; HEAT; MUTATION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RIBONUCLEASES;

EID: 0030472210     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/9.12.1197     Document Type: Article

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