An improved single-chain fab platform for efficient display and recombinant expression

Journal of Molecular Biology

Volumn 427, Issue 2, 2015, Pages 576-586

  Koerber, James T ^a,b   Hornsby, Michael J ^a,b   Wells, James A ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Antibody; Bacterial and mammalian expression; High throughput screening; Phage display; Signal peptide

Indexed keywords

RECOMBINANT ANTIBODY; SIGNAL RECOGNITION PARTICLE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; COMPLEMENTARITY DETERMINING REGION; IMMUNOGLOBULIN F(AB) FRAGMENT; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANTIBODY LIBRARY; ANTIGEN BINDING; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; DISULFIDE BOND; HIGH THROUGHPUT SEQUENCING; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SINGLE CHAIN FRAGMENT ANTIGEN BINDING; THERMOSTABILITY; AMINO ACID SEQUENCE; ANIMAL; BACTERIOPHAGE; BACTERIUM; CHEMISTRY; COMPUTER SIMULATION; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENE VECTOR; GENETICS; HEK293 CELL LINE; HUMAN; MAMMAL; MOLECULAR GENETICS; PROMOTER REGION; PROTEIN CONFORMATION; PROTEIN PROCESSING;

BACTERIA (MICROORGANISMS); MAMMALIA;

AMINO ACID SEQUENCE; ANIMALS; BACTERIA; BACTERIOPHAGES; COMPLEMENTARITY DETERMINING REGIONS; COMPUTER SIMULATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC VECTORS; HEK293 CELLS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; MAMMALS; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; PROTEIN CONFORMATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SINGLE-CHAIN ANTIBODIES;

EID: 84920755367     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2014.11.017     Document Type: Article

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