Native purification and labeling of RNA for single molecule fluorescence studies

Methods in Molecular Biology

Volumn 1240, Issue , 2015, Pages 63-95

  Rinaldi, Arlie J ^a   Suddala, Krishna C ^a   Walter, Nils G ^a  

^a NONE

Author keywords

Biotin labeling of RNA; Fluorophore labeling of RNA; Non denaturing purification; Non denaturing RNA transcription; RNA folding; Single molecule fluorescence resonance energy transfer

Indexed keywords

BIOTIN; ESTER; OLIGONUCLEOTIDE; PERIODATE; POLYNUCLEOTIDE ADENYLYLTRANSFERASE; RNA; RNA POLYMERASE; UNTRANSLATED RNA; BACTERIOPHAGE T7 RNA POLYMERASE; BENZENESULFONIC ACID DERIVATIVE; BOVINE SERUM ALBUMIN; DNA DIRECTED RNA POLYMERASE; FLUORESCENT DYE; MACROGOL DERIVATIVE; PERIODIC ACID; POLYADENYLIC ACID; RIBOSWITCH; STREPTAVIDIN; VIRUS PROTEIN;

ARTICLE; BIOTINYLATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; IN VITRO STUDY; RNA CONFORMATION; RNA FOLDING; RNA PURIFICATION; RNA STRUCTURE; RNA TRANSCRIPTION; SINGLE MOLECULE FLUORESCENCE RESONANCE ENERGY TRANSFER; ANIMAL; BOVINE; CHEMISTRY; CLICK CHEMISTRY; GENETIC TRANSCRIPTION; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; RIBOSWITCH; STAINING; THERMOANAEROBACTER;

ANIMALS; BENZENESULFONATES; BIOTINYLATION; CATTLE; CLICK CHEMISTRY; DNA-DIRECTED RNA POLYMERASES; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; OLIGONUCLEOTIDES; PERIODIC ACID; POLY A; POLYETHYLENE GLYCOLS; RIBOSWITCH; RNA; SERUM ALBUMIN, BOVINE; STAINING AND LABELING; STREPTAVIDIN; THERMOANAEROBACTER; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 84919625133     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1896-6_6     Document Type: Article

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