Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography

RNA

Volumn 16, Issue 3, 2010, Pages 647-653

  Easton, Laura E ^a   Shibata, Yoko ^a   Lukavsky, Peter J ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

Diethylaminoethyl (DEAE) sepharose; Fast performance liquid chromatography (FPLC); In vitro transcription; Native RNA purification

Indexed keywords

CHLOROFORM; EDETIC ACID; OLIGOMER; OLIGONUCLEOTIDE; PLASMID DNA; RNA; RNA POLYMERASE;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; DENATURATION; DNA TEMPLATE; PLASMID; PRIORITY JOURNAL; PROTEIN AGGREGATION; RNA PURIFICATION; RNA STRUCTURE;

CHROMATOGRAPHY, LIQUID; DNA-DIRECTED RNA POLYMERASES; RNA; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 77149143215     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.1862210     Document Type: Article

References (23)

1. Anderson AC, Scaringe SA, Earp BE, Frederick CA. 1996. HPLC purification of RNA for crystallography and NMR. RNA 2: 110-117.
2. Batey RT, Kieft JS. 2007. Improved native affinity purification of RNA. RNA 13: 1384-1389.
3. Batey RT, Inada M, Kujawinski E, Puglisi JD, Williamson JR. 1992. Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA. Nucleic Acids Res 20: 4515-4523.
4. Bullock SL, Zicha D, Ish-Horowicz D. 2003. The Drosophila hairy RNA localization signal modulates the kinetics of cytoplasmic mRNA transport. EMBO J 22: 2484-2494.
5. Doudna JA. 1997. Preparation of homogeneous ribozyme RNA for crystallization. Methods Mol Biol 74: 365-370.
6. Grodberg J, Dunn JJ. 1988. ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification. J Bacteriol 170: 1245-1253.
7. Kern JA, Davis RH. 1997. Application of solution equilibrium analysis to in vitro RNA transcription. Biotechnol Prog 13: 747-756.
8. Kieft JS, Batey RT. 2004. A general method for rapid and nondenaturing purification of RNAs. RNA 10: 988-995.
9. Kim I, McKenna SA, Viani Puglisi E, Puglisi JD. 2007. Rapid purification of RNAs using fast performance liquid chromatography (FPLC). RNA 13: 289-294.
10. LeCuyer KA, Behlen LS, Uhlenbeck OC. 1995. Mutants of the bacteriophage MS2 coat protein that alter its cooperative binding to RNA. Biochemistry 34: 10600-10606.
11. Lim F, Peabody DS. 1994. Mutations that increase the affinity of a translational repressor for RNA. Nucleic Acids Res 22: 3748-3752.
12. Lukavsky PJ, Puglisi JD. 2004. Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides. RNA 10: 889-893.
13. McKenna SA, Kim I, Puglisi EV, Lindhout DA, Aitken CE, Marshall RA, Puglisi JD. 2007. Purification and characterization of transcribed RNAs using gel filtration chromatography. Nat Protoc 2: 3270-3277.
14. Milligan JF, Groebe DR, Witherell GW, Uhlenbeck OC. 1987. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res 15: 8783-8798.
15. Pestova TV, Shatsky IN, Fletcher SP, Jackson RJ, Hellen CU. 1998. A prokaryotic-like mode of cytoplasmic eukaryotic ribosome binding to the initiation codon during internal translation initiation of hepatitis C and classical swine fever virus RNAs. Genes & Dev 12: 67-83.
16. Pikovskaya O, Serganov AA, Polonskaia A, Serganov A, Patel DJ. 2009. Preparation and crystallization of riboswitch-ligand complexes. Methods Mol Biol 540: 115-128.
17. Price SR, Ito N, Oubridge C, Avis JM, Nagai K. 1995. Crystallization of RNA-protein complexes. I. Methods for the large-scale preparation of RNA suitable for crystallographic studies. J Mol Biol 249: 398-408.
18. Serano TL, Cohen RS. 1995. A small predicted stem - loop structure mediates oocyte localization of Drosophila K10 mRNA. Development 121: 3809-3818.
19. Sharp PA. 2009. The centrality of RNA. Cell 136: 577-580.
20. Shields TP, Mollova E, Ste. Marie L, Hansen MR, Pardi A. 1999. Highperformance liquid chromatography purification of homogenouslength RNA produced by trans cleavage with a hammerhead ribozyme. RNA 5: 1259-1267.
21. Uhlenbeck OC. 1995. Keeping RNA happy. RNA 1: 4-6.
22. Winkler WC, Nahvi A, Roth A, Collins JA, Breaker RR. 2004. Control of gene expression by a natural metabolite-responsive ribozyme. Nature 428: 281-286.
23. Zhou Z, Sim J, Griffith J, Reed R. 2002. Purification and electron microscopic visualization of functional human spliceosomes. Proc Natl Acad Sci 99: 12203-12207.