Substrate binding and specificity of rhomboid intramembrane protease revealed by substrate-peptide complex structures

EMBO Journal

Volumn 33, Issue 20, 2014, Pages 2408-2421

  Zoll, Sebastian ^a,c   Stanchev, Stancho ^a   Began, Jakub ^a,b   Škerle, Jan ^a,b   Lepšík, Martin ^a   Peclinovská, Lucie ^a,b   Majer, Pavel ^a   Strisovsky, Kvido ^a  

^a INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

^c UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

intramembrane protease; rhomboid family; rhomboid protease; structure; substrate recognition

Indexed keywords

PROTEINASE; RHOMBOID PROTEASE GLPG; UNCLASSIFIED DRUG; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; GLPG PROTEIN, E COLI; MEMBRANE PROTEIN; PEPTIDE CHLOROMETHYL KETONE; PROTEIN BINDING; RECOMBINANT PROTEIN;

ARTICLE; CATALYSIS; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYMATIC ASSAY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; MOLECULAR DYNAMICS; NONHUMAN; PROTEIN FOLDING; PROVIDENCIA STUARTII; QUANTITATIVE ASSAY; SITE DIRECTED MUTAGENESIS; WATER RETENTION; ANTAGONISTS AND INHIBITORS; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; PROVIDENCIA; SYNTHESIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID CHLOROMETHYL KETONES; BINDING SITES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DNA-BINDING PROTEINS; ENDOPEPTIDASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; MUTATION; PROTEIN BINDING; PROVIDENCIA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 84911500001     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.15252/embj.201489367     Document Type: Article

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