Rapid production of novel pre-MicroRNA agent hsa-mir-27b in escherichia coli using recombinant RNA technology for functional studies in mammalian cells

Drug Metabolism and Disposition

Volumn 42, Issue 11, 2014, Pages 1791-1795

  Li, Mei Mei ^a,b   Wang, Wei Peng ^a,c   Wu, Wen Juan ^a   Huang, Min ^b   Yu, Ai Ming ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b SUN YAT SEN UNIVERSITY   (China)

^c SOOCHOW UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450 3A4; HSA MICRORNA 27B; MESSENGER RNA; MICRORNA; MICRORNA 27B; MIDAZOLAM 1' HYDROXYLASE; OXYGENASE; RECOMBINANT RNA; RECOMBINANT TRANSFER RNA; TRANSFER RNA; UNCLASSIFIED DRUG; VITAMIN D RECEPTOR; MIRN27 MICRORNA, HUMAN; PRIMER DNA; RNA;

ANION EXCHANGE FAST PROTEIN LIQUID CHROMATOGRAPHY; ARTICLE; CANCER CELL LINE; CHIMERA; CONCENTRATION RESPONSE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUNCTION; GENE FUSION; GENETIC PROCEDURES; HUMAN; HUMAN CELL; LIQUID CHROMATOGRAPHY; LS 180 CELL LINE; MAMMAL CELL; PROTEIN EXPRESSION; PURIFICATION; RECOMBINANT RNA TECHNOLOGY; RNA PROCESSING; RNA SYNTHESIS; BIOSYNTHESIS; GENETIC RECOMBINATION; GENETICS; ION EXCHANGE CHROMATOGRAPHY; NUCLEOTIDE SEQUENCE; TUMOR CELL LINE;

BASE SEQUENCE; CELL LINE, TUMOR; CHROMATOGRAPHY, ION EXCHANGE; DNA PRIMERS; ESCHERICHIA COLI; HUMANS; MICRORNAS; RECOMBINATION, GENETIC; RNA;

EID: 84907917954     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.114.060145     Document Type: Article

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