A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold

Nature Protocols

Volumn 4, Issue 6, 2009, Pages 947-959

  Ponchon, Luc ^a   Beauvais, Geneviève ^a   Nonin Lecomte, Sylvie ^a   Dardel, Frédéric ^a  

^a UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA; RECOMBINANT RNA; RIBONUCLEASE H; TRANSFER RNA;

ARTICLE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE FUSION; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; RNA ANALYSIS; RNA CLEAVAGE; RNA PURIFICATION; RNA STABILITY; RNA STRUCTURE; RNA VECTOR; STRUCTURE ANALYSIS;

BASE SEQUENCE; CHROMATOGRAPHY, LIQUID; ESCHERICHIA COLI; GENETIC TECHNIQUES; GENETIC VECTORS; ISOTOPES; MOLECULAR SEQUENCE DATA; PLASMIDS; RIBONUCLEASE H; RNA; RNA, BACTERIAL; RNA, TRANSFER;

ESCHERICHIA COLI;

EID: 66749152587     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.67     Document Type: Article

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