Thermoase-derived flaxseed protein hydrolysates and membrane ultrafiltration peptide fractions have systolic blood pressure-lowering effects in spontaneously hypertensive rats

International Journal of Molecular Sciences

Volumn 15, Issue 10, 2014, Pages 18131-18147

  Nwachukwu, Ifeanyi D ^a   Girgih, Abraham T ^b   Malomo, Sunday A ^a   Onuh, John O ^a   Aluko, Rotimi E ^a,b  

^a UNIVERSITY OF MANITOBA   (Canada)

^b UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Angiotensin converting enzyme; Antihypertensive; Bioactive peptides; Flaxseed; Hypertension; Membrane ultrafiltration; Renin; Spontaneously hypertensive rat; Systolic blood pressure; Thermoase

Indexed keywords

BACTERIAL ENZYME; CAPTOPRIL; DIPEPTIDYL CARBOXYPEPTIDASE; FLAXSEED PROTEIN; PROTEIN HYDROLYSATE; RENIN; THERMOASE; UNCLASSIFIED DRUG; VEGETABLE PROTEIN; ANTIHYPERTENSIVE AGENT; DIPEPTIDYL CARBOXYPEPTIDASE INHIBITOR; PLANT EXTRACT;

AMINO ACID COMPOSITION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIHYPERTENSIVE ACTIVITY; ARTICLE; BLOOD PRESSURE REGULATION; CONTROLLED STUDY; DRUG PURIFICATION; ENZYME DIGESTION; ENZYME INHIBITION; EXTRACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IN VITRO STUDY; IN VIVO STUDY; LINSEED; MALE; MEMBRANE FILTER; NONHUMAN; PROTEIN HYDROLYSIS; RAT; SPONTANEOUSLY HYPERTENSIVE RAT; SYSTOLIC BLOOD PRESSURE; ULTRAFILTRATION; ANIMAL; ANTAGONISTS AND INHIBITORS; BLOOD PRESSURE; CHEMISTRY; DRUG EFFECTS; FLAX; HYPERTENSION; ISOLATION AND PURIFICATION; PATHOPHYSIOLOGY; PLANT SEED;

RATTUS;

ANGIOTENSIN-CONVERTING ENZYME INHIBITORS; ANIMALS; ANTIHYPERTENSIVE AGENTS; BLOOD PRESSURE; FLAX; HUMANS; HYPERTENSION; MALE; PLANT EXTRACTS; PLANT PROTEINS; RATS, INBRED SHR; RENIN; SEEDS; ULTRAFILTRATION;

EID: 84907835503     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms151018131     Document Type: Article

References (32)

1. Lawes, C. M.; Hoorn, S. V.; Rodgers, A. Global burden of blood-pressure-related disease, 2001. Lancet 2008, 371, 1513-1518.
2. Lim, S. S.; Vos, T.; Flaxman, A. D.; Danaei, G.; Shibuya, K.; Adair-Rohani, H.; AlMazroa, M. A.; Amann, M.; Anderson, H. R.; Andrews, K. G.; et al. A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990-2010: A systematic analysis for the global burden of disease study 2010. Lancet 2012, 380, 2224-2260.
3. Atlas, S. A. The renin-angiotensin aldosterone system: Pathophysiological role and pharmacologic inhibition. J. Manag. Care Pharm. 2007, 13, S9-S20.
4. Chen, J.; Liu, S.; Ye, R.; Cai, G.; Ji, B.; Wu, Y. Angiotensin-I converting enzyme (ACE) inhibitory tripeptides from rice protein hydrolysate: Purification and characterization. J. Funct. Foods 2013, 5, 1684-1692.
5. Fengjuan, L.; Takahashi, Y.; Yamaki, K. Inhibitory effect of catechin-related compounds on renin activity. Biomed. Res. 2013, 34, 167-171.
6. Udenigwe, C. C.; Lin, Y.; Hou, W.; Aluko, R. E. Kinetics of the inhibition of renin and angiotensin I-converting enzyme by flaxseed protein hydrolysate fractions. J. Funct. Food. 2009, 1, 199-207.
7. Yang, H. Y. T.; Erdös, E. G.; Levin, Y. A dipeptidyl carboxypeptidase that converts angiotensin-I and inactivates bradykinin. Biochim. Biophys. Acta 1970, 214, 374-376.
8. Segall, L.; Covic, A.; Goldsmith, D. J. A. Direct renin inhibitors: the dawn of a new era, or just a variation on a theme? Nephrol. Dial. Transpl. 2007, 22, 2435-2439.
9. Girgih, A. T.; Udenigwe, C. C.; Li, H.; Adebiyi, A. P.; Aluko, R. E. Kinetics of enzyme inhibition and antihypertensive effects of hemp seed (Cannabis sativa L.) protein hydrolysates. J. Am. Oil Chem. Soc. 2011, 88, 1767-1774.
10. Mäkinen, S.; Johannson, T.; Vegarud Gerd, E.; Pihlava, J. M.; Pihlanto, A. Angiotensin I-converting enzyme inhibitory and antioxidant properties of rapeseed hydrolysates. J. Funct. Foods 2012, 4, 575-583.
11. Martínez-Maqueda, D.; Miralles, B.; Recio, I.; Hernández-Ledesma, B. Antihypertensive peptides from food proteins: A review. Food Funct. 2012, 3, 350-361.
12. Onuh, J. O.; Girgih, A. T.; Aluko, R. E.; Aliani, M. Inhibitions of renin and angiotensin converting enzyme activities by enzymatic chicken skin protein hydrolysates. Food Res. Int. 2013, 53, 260-267.
13. Hall, C., III; Tulbek, M. C.; Xu, Y. Flaxseed. Adv. Food Nutr. Res. 2006, 51, 1-97.
14. Doyen, A.; Udenigwe, C. C.; Mitchell, P. L.; Marette, A.; Aluko, R. E.; Bazinet, L. Anti-diabetic and antihypertensive activities of two flaxseed protein hydrolysate fractions revealed following their simultaneous separation by electrodialysis with ultrafiltration membranes. Food Chem. 2014, 145, 66-76.
15. Marambe, P. W. M. L. H. K.; Shand, P. J.; Wanasundara, J. P. D. An in vitro investigation of selected biological activities of hydrolysed flaxseed (Linum usitatissimum L.) proteins. J. Am. Oil Chem. Soc. 2008, 85, 1155-1164.
16. Xu, Y.; Hall, C., III; Wolf-Hall, C. Antifungal activity stability of flaxseed protein extract using response surface methodology. J. Food Sci. 2008, 73, M9-M14.
17. Oomah, B. D.; Mazza, G. Functional properties, uses of flaxseed protein. Inform 1995, 6, 1246-1252.
18. Udenigwe, C. C.; Aluko, R. E. Antioxidant and angiotensin converting enzyme-inhibitory properties of a flaxseed protein-derived high fischer ratio peptide mixture. J. Agric. Food Chem. 2010, 58, 4762-4768.
19. Keil, B. Specificity of Proteolysis; Springer-Verlag: Berlin, Germany, 1992; p. 336.
20. Alashi, A. M.; Blanchard, C. L.; Mailer, R. J.; Agboola, S. O.; Mawson, A. J.; He, R.; Malomo, S. A.; Girgih, A. T.; Aluko, R. E. Blood pressure lowering effects of Australian canola protein hydrolysates in spontaneously hypertensive rats. Food Res. Int. 2014, 55, 281-287.
21. He, R.; Alashi, A.; Malomo, S. A.; Girgih, A. T.; Chao, D.; Ju, X.; Aluko, R. E. Antihypertensive and free radical scavenging properties of enzymatic rapeseed protein hydrolysates. Food Chem. 2013, 141, 153-159.
22. Ajibola, C. F.; Fashakin, J. B.; Fagbemi, T. N.; Aluko, R. E. Renin and angiotensin converting enzyme inhibition with antioxidant properties of african yam bean protein hydrolysate and reverse-phase HPLC-separated peptide fractions. Food Res. Int. 2013, 52, 437-444.
23. Udenigwe, C. C.; Adebiyi, A. P.; Doyen, A.; Li, H.; Bazinet, L.; Aluko, R. E. Low molecular weight flaxseed protein-derived arginine-containing peptides reduced blood pressure of spontaneously hypertensive rats faster than amino acid form of arginine and native flaxseed protein. Food Chem. 2012, 132, 468-475.
24. Wu, J.; Aluko, R. E.; Nakai, S. Structural requirements of angiotensin I-converting enzyme inhibitory peptides: Quantitative structure-activity relationship study of di-and tripeptides. J. Agric. Food Chem. 2006, 54, 732-738.
25. Ishiguro, K.; Sameshima, Y.; Kume, T.; Ikeda, K.; Matsumoto, J.; Yoshimoto, M. Hypotensive effect of a sweetpotato protein digest in spontaneously hypertensive rats and purification of angiotensin I-converting enzyme inhibitory peptides. Food Chem. 2012, 131, 774-779.
26. Dev, D. K.; Quensel, E. Preparation and functional properties of linseed protein products containing differing levels of mucilage. J. Food Sci. 1988, 53, 1834-1837.
27. Markwell, M. A. K.; Haas, S. M.; Bieber, L. L.; Tolbert, N. E. A modification of the lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 1978, 87, 206-210.
28. Holmquist, B.; Bunning, P.; Riordan, J. F. A continuous spectrophotometric assay for angiotensin converting enzyme. Anal. Biochem. 1979, 95, 540-548.
29. Bidlingmeyer, B. A.; Cohen, S. A.; Tarvin, T. L. Rapid analysis of amino acids using pre-column derivatization. J. Chromatogr. Biomed. Appl. 1984, 336, 93-104.
30. Gehrke, C. W.; Wall Sr., L. L.; Absheer, J. S. Sample preparation for chromatography of amino acids: Acid hydrolysis of proteins. J. Assoc. Off. Anal. Chem. 1985, 68, 811-821.
31. Landry, J.; Delhaye, S. Simplified procedure for the determination of tryptophan of foods and feedstuffs from barytic hydrolysis. J. Agric. Food Chem. 1992, 40, 776-779.
32. Aukema, H. M.; Gauthier, J.; Roy, M.; Jia, Y.; Li, H.; Aluko, R. E. Distinctive effects of plant protein sources on renal disease progression and associated cardiac hypertrophy in experimental kidney disease. Mol. Nutr. Food Res. 2011, 55, 1044-1051.