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Bioorganic and Medicinal Chemistry Letters
Volumn 24, Issue 18, 2014, Pages 4389-4396
Discovery of a new family of carbonic anhydrases in the malaria pathogen Plasmodium falciparum - The η-carbonic anhydrases
Author keywords

Anion; Carbonic anhydrase; Inhibitor; Plasmodium falciparum; CA class enzyme
Indexed keywords

BENZENE DERIVATIVE; BENZENEBORONIC ACID; CARBON DIOXIDE; CARBONATE DEHYDRATASE; ETA CARBONIC ANHYDRASE; PHENYLARSONIC ACID; PROTOZOAL PROTEIN; SULFAMIC ACID; SULFANILAMIDE; SULFUR ACID DERIVATIVE; UNCLASSIFIED DRUG; CARBONATE DEHYDRATASE INHIBITOR; ISOENZYME; ORGANOMETALLIC COMPOUND; ZINC;
EID: 84906960520     PISSN: 0960894X     EISSN: 14643405     Source Type: Journal    
DOI: 10.1016/j.bmcl.2014.08.015     Document Type: Article
Times cited : (295)
References (78)
  • 64
    • 0015239422 scopus 로고
    • An applied photophysics stopped-flow instrument has been used for assaying the CA catalyzed CO2hydration activity Phenol red (at a concentration of 0.2 mM) has been used as indicator
    • working at the absorbance maximum of 557 nm, with 10-20 mM Hepes (pH 7.5) as buffer, and 20 mM Na2SO4or 20 mM NaBF4for maintaining constant the ionic strength, following the initial rates of the CA-catalyzed CO2hydration reaction for a period of 10-100 s. The CO2concentrations ranged from 1.7 to 17 mM for the determination of the kinetic parameters and inhibition constants. For each inhibitor at least six traces of the initial 5-10% of the reaction have been used for determining the initial velocity. The uncatalyzed rates were determined in the same manner and subtracted from the total observed rates. Stock solutions of inhibitor (10 mM) were prepared in distilled-deionized water and dilutions up to 0.01 μM were done thereafter with distilled-deionized water. Inhibitor and enzyme solutions were preincubated together for 15 min at room temperature prior to assay, in order to allow for the formation of the E-I complex.
    • R.G. Khalifah J. Biol. Chem. 246 1971 2561 An applied photophysics stopped-flow instrument has been used for assaying the CA catalyzed CO2hydration activity. Phenol red (at a concentration of 0.2 mM) has been used as indicator, working at the absorbance maximum of 557 nm, with 10-20 mM Hepes (pH 7.5) as buffer, and 20 mM Na2SO4or 20 mM NaBF4for maintaining constant the ionic strength, following the initial rates of the CA-catalyzed CO2hydration reaction for a period of 10-100 s. The CO2concentrations ranged from 1.7 to 17 mM for the determination of the kinetic parameters and inhibition constants. For each inhibitor at least six traces of the initial 5-10% of the reaction have been used for determining the initial velocity. The uncatalyzed rates were determined in the same manner and subtracted from the total observed rates. Stock solutions of inhibitor (10 mM) were prepared in distilled-deionized water and dilutions up to 0.01 μM were done thereafter with distilled-deionized water. Inhibitor and enzyme solutions were preincubated together for 15 min at room temperature prior to assay, in order to allow for the formation of the E-I complex. The inhibition constants were obtained by non-linear least-squares methods using the Cheng-Prusoff equation whereas the kinetic parameters for the uninhibited enzymes from Lineweaver-Burk plots, as reported earlier, 29-31and represent the mean from at least three different determinations. PfCA was a recombinant protein, obtained and purified as reported earlier.23a
    • (1971) J. Biol. Chem. , vol.246 , pp. 2561
    • Khalifah, R.G.1

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.