Reduction in C-terminal amidated species of recombinant monoclonal antibodies by genetic modification of CHO cells

BMC Biotechnology

Volumn 14, Issue 1, 2014, Pages

  Sˇkulj, Mihaela ^a   Pezdirec, Dejan ^a   Gaser, Dominik ^a   Kreft, Marko ^b,c,d   Zorec, Robert ^b,c  

^a Lek Pharmaceuticals Dd   (Slovenia)

^b Celica Biomedical Sciences Center   (Slovenia)

^c UNIVERSITY OF LJUBLJANA   (Slovenia)

^d UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

C terminal amidation; Genetic modification; Recombinant monoclonal antibody

Indexed keywords

AMIDATION; CHO CELL; GENETIC MODIFICATIONS; RECOMBINANT MONOCLONAL ANTIBODY;

ALPHA AMIDATING ENZYME; MESSENGER RNA; MONOCLONAL ANTIBODY; PLASMID DNA; RNA; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; ZINC FINGER NUCLEASE; MIXED FUNCTION OXIDASE; MULTIENZYME COMPLEX; PEPTIDYLGLYCINE MONOOXYGENASE; RECOMBINANT PROTEIN;

AMIDATION; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL CLONE; CHO CELL LINE; CONTROLLED STUDY; DNA MODIFICATION; FEMALE; GENE DISRUPTION; GENE EXPRESSION; GENE FREQUENCY; GENE SILENCING; GENE TARGETING; GENETIC MANIPULATION; NONHUMAN; PROTEIN STABILITY; REDUCTION; RNA INTERFERENCE; ANIMAL; BIOSYNTHESIS; CHEMISTRY; CRICETULUS; GENETIC ENGINEERING; GENETICS; METHODOLOGY; PROTEIN PROCESSING;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETULUS; GENETIC ENGINEERING; MIXED FUNCTION OXYGENASES; MULTIENZYME COMPLEXES; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; RNA INTERFERENCE;

EID: 84906838429     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-14-76     Document Type: Article

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