Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution

Biotechnology Journal

Volumn 9, Issue 8, 2014, Pages 1013-1022

  Traxlmayr, Michael W ^a   Lobner, Elisabeth ^a   Hasenhindl, Christoph ^a   Stadlmayr, Gerhard ^a   Oostenbrink, Chris ^a   Rüker, Florian ^a   Obinger, Christian ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Antibody engineering; Directed evolution; Fcab; PH depending binding; Yeast surface display

Indexed keywords

AMINO ACIDS; ANTIGENS; BODY FLUIDS; YEAST;

ANTIBODY ENGINEERING; DIRECTED EVOLUTION; FCAB; PH-DEPENDING BINDING; YEAST SURFACE DISPLAYS;

PROTEINS;

EPIDERMAL GROWTH FACTOR RECEPTOR 2; FC RECEPTOR; HISTIDINE; IMMUNOGLOBULIN G1; PROTEIN VARIANT; ERBB2 PROTEIN, HUMAN; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G;

ANTIBODY ENGINEERING; ANTIBODY LIBRARY; ANTIGEN BINDING; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTALLIZATION; DIRECTED MOLECULAR EVOLUTION; GEL PERMEATION CHROMATOGRAPHY; MOLECULAR DYNAMICS; MUTATION; NONHUMAN; PH; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STABILITY; SCREENING; THERMOSTABILITY; CHEMISTRY; HUMAN; METABOLISM; PHYSIOLOGY; PICHIA; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE;

BINDING SITES; DIRECTED MOLECULAR EVOLUTION; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MOLECULAR DYNAMICS SIMULATION; PICHIA; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RECEPTOR, ERBB-2;

EID: 84905222360     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300483     Document Type: Article

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