A combinatorial histidine scanning library approach to engineer highly pH-dependent protein switches

Protein Science

Volumn 20, Issue 9, 2011, Pages 1619-1631

  Murtaugh, Megan L ^a   Fanning, Sean W ^a   Sharma, Tressa M ^a   Terry, Alexandra M ^a   Horn, James R ^a  

^a NORTHERN ILLINOIS UNIVERSITY   (United States)

Author keywords

Camelid VHH; Combinatorial histidine scanning; Dual function; Histidine; pH switch; Phage display; pK a; Protein engineering; Single domain antibody

Indexed keywords

HISTIDINE; PROTEIN; PROTEIN ANTIBODY; RIBONUCLEASE A; UNCLASSIFIED DRUG; VHH ANTIBODY;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; COMBINATORIAL LIBRARY; CONTROLLED STUDY; IN VITRO STUDY; IONIZATION; NONHUMAN; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; WILD TYPE;

HISTIDINE; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEINS; THERMODYNAMICS;

CAMELIDAE;

EID: 84860388904     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.696     Document Type: Article

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