Creating stable stem regions for loop elongation in Fcabs - Insights from combining yeast surface display, in silico loop reconstruction and molecular dynamics simulations

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1844, Issue 9, 2014, Pages 1530-1540

  Hasenhindl, Christoph ^a   Lai, Balder ^a   Delgado, Javier ^b   Traxlmayr, Michael W ^a   Stadlmayr, Gerhard ^a   Rüker, Florian ^a   Serrano, Luis ^b   Oostenbrink, Chris ^a   Obinger, Christian ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b CENTRE FOR GENOMIC REGULATION CRG   (Spain)

Author keywords

Fcab; Loop reconstruction; Molecular dynamics simulation; Protein engineering; Therapeutic antibody fragment; Yeast surface display

Indexed keywords

FC ANTIGEN BINDING PROTEIN; IMMUNOGLOBULIN; IMMUNOGLOBULIN FC FRAGMENT; UNCLASSIFIED DRUG;

ARTICLE; COMPUTER MODEL; DIFFERENTIAL SCANNING CALORIMETRY; FLOW CYTOMETRY; MOLECULAR DYNAMICS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; THERMOSTABILITY; WILD TYPE; YEAST;

FCAB; LOOP RECONSTRUCTION; MOLECULAR DYNAMICS SIMULATION; PROTEIN ENGINEERING; THERAPEUTIC ANTIBODY FRAGMENT; YEAST SURFACE DISPLAY;

CELL SURFACE DISPLAY TECHNIQUES; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MOLECULAR DYNAMICS SIMULATION; MUTATION; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE;

EID: 84902281027     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2014.04.020     Document Type: Article

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