Rational cytokine design for increased lifetime and enhanced potency using pH-activated “histidine switching”

Nature Biotechnology

Volumn 20, Issue 9, 2002, Pages 908-913

  Sarkar, Casim A ^a,b   Lowenhaupt, Ky ^b   Horan, Thomas ^d   Boone, Thomas C ^d   Tidor, Bruce ^b,c   Lauffenburger, Douglas A ^a,b  

^a USA   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c NONE   (United States)

^d AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; BIOASSAY; CELLS; PROTEINS; RECYCLING;

ENDOSOMAL COMPARTMENTS;

PH EFFECTS;

CYTOKINE; HISTIDINE; PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; COMPUTER AIDED DESIGN; COMPUTER PREDICTION; CYTOKINE PRODUCTION; ELECTRICITY; GENE SWITCHING; HALF LIFE TIME; LIGAND BINDING; MATHEMATICAL COMPUTING; MOLECULAR MODEL; PH; PRIORITY JOURNAL; PROTON TRANSPORT; RECEPTOR BINDING; RECYCLING;

COMPUTER SIMULATION; CYTOKINES; ELECTROSTATICS; ESCHERICHIA COLI; GENES, SWITCH; GRANULOCYTE COLONY-STIMULATING FACTOR; HISTIDINE; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECEPTORS, GRANULOCYTE COLONY-STIMULATING FACTOR; SENSITIVITY AND SPECIFICITY;

EID: 0036713917     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt725     Document Type: Article

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