Novel 1:1 Labeling and purification process for C-terminal thioester and single cysteine recombinant proteins using generic peptidic toolbox reagents

Bioconjugate Chemistry

Volumn 25, Issue 7, 2014, Pages 1213-1222

  Portal, Christophe F ^a   Seifert, Jan Marcus ^b,d   Buehler, Christof ^b,e   Meisner Kober, Nicole Claudia ^b,c   Auer, Manfred ^a,b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b Novartis Institutes for Biomedical Research Vienna   (Austria)

^c NOVARTIS PHARMA AG   (Switzerland)

^d Marinomed Biotechnologie GmbH   (Austria)

^e Supercomputing Systems AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; ENERGY TRANSFER; FLUORESCENCE IMAGING; FLUORESCENCE SPECTROSCOPY; MASS SPECTROMETRY; MOLECULES; PURIFICATION; RECOMBINANT PROTEINS; SOLUBILITY; SPECTROSCOPIC ANALYSIS; SPECTRUM ANALYSIS; STOICHIOMETRY;

FLUCTUATION ANALYSIS; FLUORESCENCE FLUCTUATION; FLUORESCENT PROTEIN; LABELINGS; LABELLED PROTEIN; PROTEIN COMPLEXES; PURIFICATION PROCESS; SIMPLE++; SINGLE-MOLECULE RESOLUTION; TARGET PROTEINS;

AMINO ACIDS;

CYSTEINE; ESTER; FLUORESCENT DYE; MALEIMIDE DERIVATIVE; RECOMBINANT PROTEIN; RNA; THIOESTER; ELAV PROTEIN; PEPTIDE FRAGMENT; SULFUR DERIVATIVE;

ANALYTIC METHOD; ARTICLE; BIOLOGY; CARBOXY TERMINAL SEQUENCE; CHEMICAL LABELING; CHEMICAL REACTION; FLUORESCENCE CORRELATION SPECTROSCOPY; FLUORESCENCE CROSS CORRELATION SPECTROSCOPY; FLUORESCENCE IMAGING; FLUORESCENCE INTENSITY DIFFUSION ANALYSIS; FLUORESCENCE SPECTROSCOPY; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; LIGAND BINDING; MASS SPECTROMETRY; MOLECULAR RECOGNITION; PROTEIN FUNCTION; PURIFICATION; QUANTITATIVE ANALYSIS; RNA BINDING; SOLUBILITY; SPECTROSCOPY; STOICHIOMETRY; AFFINITY CHROMATOGRAPHY; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; SPECTROFLUOROMETRY;

CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; ELAV PROTEINS; FLUORESCENT DYES; HUMANS; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; RNA; SPECTROMETRY, FLUORESCENCE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SULFUR COMPOUNDS;

EID: 84904419402     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/bc5000059     Document Type: Article

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