Selective N-terminal fluorescent labeling of proteins using 4-chloro-7-nitrobenzofurazan: A method to distinguish protein N-terminal acetylation

Analytical Biochemistry

Volumn 428, Issue 1, 2012, Pages 13-15

  Bernal Perez, Lina F ^a   Prokai, Laszlo ^b   Ryu, Youngha ^a  

^a TEXAS CHRISTIAN UNIVERSITY   (United States)

^b UNIVERSITY OF NORTH TEXAS   (United States)

Author keywords

Fluorescent labeling; NBD Cl; Protein N terminal acetylation; Thymosin; Z domain

Indexed keywords

ACETYLATION; AMINO ACIDS; FLUORESCENCE;

4-CHLORO-7-NITROBENZOFURAZAN; DERIVATIZATION METHOD; FLUORESCENT LABELING; MICROMOLAR CONCENTRATION; PROTEIN N-TERMINAL ACETYLATION; RELIABLE DETECTION; THYMOSIN; Z-DOMAIN;

PROTEINS;

4 CHLORO 7 NITROBENZOFURAZAN; FLUORESCENT DYE; THYMOSIN ALPHA1;

ACETYLATION; AMINO TERMINAL SEQUENCE; ARTICLE; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MODIFICATION; PROTEIN N TERMINAL ACETYLATION;

EID: 84863097876     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.05.026     Document Type: Article

References (27)

1. C. Walsh Posttranslational Modification of Proteins: Expanding Nature's Inventory 2006 Roberts and CO. Englewood
2. D.C. Scott, J.K. Monda, E.J. Bennett, J.W. Harper, and B.A. Schulman N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex Science 334 2011 674 678
3. T. Arnesen Towards a functional understanding of protein N-terminal acetylation PLoS Biol. 9 2011 e1001074
4. J.L. Andersen, and S. Kornbluth Meeting the (N-terminal) end with acetylation Cell 146 2011 503 505
5. C.S. Hwang, A. Shemorry, and A. Varshavsky N-terminal acetylation of cellular proteins creates specific degradation signals Science 327 2010 973 977
6. S.R. Van Doren, S. Wei, G. Gao, B.B. DaGue, M.O. Palmier, H. Bahudhanapati, and K. Brew Inactivation of N-TIMP-1 by N-terminal acetylation when expressed in bacteria Biopolymers 89 2008 960 968
7. Z. Luka, L.V. Loukachevitch, and C. Wagner Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate Biochim. Biophys. Acta 1784 2008 1342 1346
8. C.L. Jackson N-terminal acetylation targets GTPases to membranes Nat. Cell Biol. 6 2004 379 380
9. H. Ogawa, T. Gomi, Y. Takata, T. Date, and M. Fujioka Recombinant expression of rat glycine N-methyltransferase and evidence for contribution of N-terminal acetylation to co-operative binding of S-adenosylmethionine Biochem. J. 327 1997 407 412 (Pubitemid 27477815)
10. M. Urbancikova, and S.E. Hitchcock-DeGregori Requirement of amino-terminal modification for striated muscle α-tropomyosin function J. Biol. Chem. 269 1994 24310 24315
11. R.J. Arnold, and J.P. Reilly Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry Anal. Biochem. 269 1999 105 112 (Pubitemid 29174267)
12. V.F. Smith, B.L. Schwartz, L.L. Randall, and R.D. Smith Electrospray mass spectrometric investigation of the chaperone SecB Protein Sci. 5 1996 488 494 (Pubitemid 26081626)
13. K. Arai, B.F.C. Clark, L. Duffy, M.D. Jones, Y. Kaziro, R.A. Laursen, J. Litalien, D.L. Miller, S. Nagarkatti, S. Nakamura, K.M. Nielsen, T.E. Petersen, K. Takahashi, and M. Wade Primary structure of elongation-factor Tu from Escherichia coli Proc. Natl. Acad. Sci. USA 77 1980 1326 1330 (Pubitemid 10072281)
14. A. Yoshikawa, S. Isono, A. Sheback, and K. Isono Cloning and nucleotide sequencing of the genes RimI and RimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12 Mol. Gen. Genet. 209 1987 481 488
15. S. Tanaka, Y. Matsushita, A. Yoshikawa, and K. Isono Cloning and molecular characterization of the gene RimL which encodes an enzyme acetylating ribosomal protein L12 of Escherichia coli K12 Mol. Gen. Genet. 217 1989 289 293 (Pubitemid 19165009)
16. E. Charbaut, V. Redeker, J. Rossier, and A. Sobel N-terminal acetylation of ectopic recombinant proteins in Escherichia coli FEBS Lett. 529 2002 341 345
17. α-acylated Arch. Biochem. Biophys. 269 1989 612 622 (Pubitemid 19075771)
18. J. Wu, S. Chang, X. Gong, D. Liu, and Q. Ma Identification of N-terminal acetylation of recombinant human prothymosin alpha in Escherichia coli Biochim. Biophys. Acta 1760 2006 1241 1247 (Pubitemid 43949438)
19. M.G. Grutter, W. Marki, and H.P. Walliser Crystals of the complex between recombinant N-acetyleglin c and subtilisin: a preliminary characterization J. Biol. Chem. 260 1985 1436 1437
20. T. Takao, M. Kobayashi, O. Nishimura, and Y. Shimonishi Chemical characterization of recombinant human leukocyte interferon A using fast atom bombardment mass spectrometry J. Biol. Chem. 262 1987 3541 3547 (Pubitemid 17136447)
21. α-acetylated Y-pestis fusion protein F1-V expressed in Escherichia coli using LCMSE J. Biotechnol. 130 2007 11 23 (Pubitemid 46634650)
22. α-acetylated thymosin α1 in Escherichia coli Microb. Cell Fact. 10 2011 26
23. α-acetylation of thymosin α1 in Escherichia coli Appl. Microbiol. Biotechnol. 84 2009 99 104
24. L.F. Bernal-Perez, F. Sahyouni, L. Prokai, and Y. Ryu RimJ-mediated context-dependent N-terminal acetylation of the recombinant Z-domain protein in Escherichia coli Mol. Biosyst. 8 2012 1128 1130
25. S.S. Simons, and D.F. Johnson Reaction of ortho-phthalaldehyde and thiols with primary amines: Fluorescence properties of 1-alkyl (and aryl)thio-2-alkylisoindoles Anal. Biochem. 90 1978 705 725 (Pubitemid 9087723)
26. D.Y. Zheng, J.M. Aramini, and G.T. Montelione Validation of helical tilt angles in the solution NMR structure of the Z domain of staphylococcal protein A by combined analysis of residual dipolar coupling and NOE data Protein Sci. 13 2004 549 554 (Pubitemid 38124976)
27. P.B. Ghosh, and M.W. Whitehouse 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines Biochem. J. 108 1968 155 156