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Volumn 128, Issue 32, 2006, Pages 10452-10459

Oligo-Asp Tag/Zn(II) complex probe as a new pair for labeling and fluorescence imaging of proteins

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL BONDS; COMPLEXATION; FLUORESCENCE; IMAGING TECHNIQUES; TITRATION; ZINC;

EID: 33747613117     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0618604     Document Type: Article
Times cited : (150)

References (38)
  • 20
    • 33747597903 scopus 로고    scopus 로고
    • note
    • n-EEEE-, where X is any amino acid and n = 0, 1, or 2) is 158, which is much larger than that of the corresponding oligo-aspartate sequence. This is also an advantage of the oligo-aspartate tag.
  • 22
    • 33747620184 scopus 로고    scopus 로고
    • note
    • The binding between 1-2Zn(II) (25 μM) and the D4 peptide was also evaluated by ITC under slightly acidic conditions (50 mM MES buffer, pH 5.5). Heat evolution due to the complex formation ceased sharply after the addition of 1.5 equiv of the D4 peptide. This suggests that the binding affinity of 1-2Zn(II) for the D4 peptide is partially maintained under the acidic conditions, though detailed thermodynamic analysis cannot be done because the resultant binding isotherm is biphasic.
  • 23
    • 33747603105 scopus 로고    scopus 로고
    • note
    • -1).
  • 26
    • 33747620623 scopus 로고    scopus 로고
    • note
    • -1).
  • 29
    • 33747601743 scopus 로고    scopus 로고
    • note
    • The in vitro fluorescence titration experiment confirmed that the fluorescence intensity of Cy5-3-4Zn(II) scarcely changed upon binding to the D4-G-D4 peptide.
  • 34
    • 33747602894 scopus 로고    scopus 로고
    • note
    • Since FITC-2-4Zn(II) and Cy5-3-4Zn(II) are not membrane-permeable, they are useful for the labeling of cell-surface membrane receptor. On the other hand, we found that the fluorescent DpaTyr dimers, having a BODIPY or a rhodamine derivative, can penetrate through the cell membrane so that these derivatives localize in the cytoplasm of CHO cell.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.