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The observed increase of the mass number 755 is smaller than the theoretical value 986 (2-2Zn(II)-HC1-3C1). This shortage probably comes from the decomplexation of the Zn(II) ion(s) and/or the cleavage of the dipicolylamine (Dpa) fragment from the probe owing to the acidic pretreatment of the sample for desalting and harsh laser-induced ionization of the labeled protein in the MS analysis. The same shortage was observed in MALDI-TOF mass analysis of 1-2Zn(II).
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The observed increase of the mass number 755 is smaller than the theoretical value 986 (2-2Zn(II)-HC1-3C1). This shortage probably comes from the decomplexation of the Zn(II) ion(s) and/or the cleavage of the dipicolylamine (Dpa) fragment from the probe owing to the acidic pretreatment of the sample for desalting and harsh laser-induced ionization of the labeled protein in the MS analysis. The same shortage was observed in MALDI-TOF mass analysis of 1-2Zn(II).
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Bräse, S.; Gil, C.; Knepper, K.; Zimmermann, V. Angew. Chem., Int. Ed. 2005, 44, 5188.
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Zimmermann, V.4
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The observed fluorescence only derives from the coumarin derivative 4 introduced into EGFP and not from EGFP. During the pretreatment for SDS-PAGE (95°C, 3min), EGFP completely lost its fluorescence.
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The observed fluorescence only derives from the coumarin derivative 4 introduced into EGFP and not from EGFP. During the pretreatment for SDS-PAGE (95°C, 3min), EGFP completely lost its fluorescence.
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d = 1.4 μM).
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d = 1.4 μM).
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