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Volumn 129, Issue 51, 2007, Pages 15777-15779

Non-enzymatic covalent protein labeling using a reactive tag

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ASPARTIC ACID; CYSTEINE; ENHANCED GREEN FLUORESCENT PROTEIN; GLUTATHIONE TRANSFERASE; HEMOGLOBIN; PROTEIN; SERUM ALBUMIN; THIOL; THIOREDOXIN; TYROSINE; ZINC;

EID: 37549061836     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja074176d     Document Type: Article
Times cited : (91)

References (27)
  • 24
    • 37549029131 scopus 로고    scopus 로고
    • The observed increase of the mass number 755 is smaller than the theoretical value 986 (2-2Zn(II)-HC1-3C1). This shortage probably comes from the decomplexation of the Zn(II) ion(s) and/or the cleavage of the dipicolylamine (Dpa) fragment from the probe owing to the acidic pretreatment of the sample for desalting and harsh laser-induced ionization of the labeled protein in the MS analysis. The same shortage was observed in MALDI-TOF mass analysis of 1-2Zn(II).
    • The observed increase of the mass number 755 is smaller than the theoretical value 986 (2-2Zn(II)-HC1-3C1). This shortage probably comes from the decomplexation of the Zn(II) ion(s) and/or the cleavage of the dipicolylamine (Dpa) fragment from the probe owing to the acidic pretreatment of the sample for desalting and harsh laser-induced ionization of the labeled protein in the MS analysis. The same shortage was observed in MALDI-TOF mass analysis of 1-2Zn(II).
  • 26
    • 37549070999 scopus 로고    scopus 로고
    • The observed fluorescence only derives from the coumarin derivative 4 introduced into EGFP and not from EGFP. During the pretreatment for SDS-PAGE (95°C, 3min), EGFP completely lost its fluorescence.
    • The observed fluorescence only derives from the coumarin derivative 4 introduced into EGFP and not from EGFP. During the pretreatment for SDS-PAGE (95°C, 3min), EGFP completely lost its fluorescence.
  • 27
    • 37549005933 scopus 로고    scopus 로고
    • d = 1.4 μM).
    • d = 1.4 μM).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.