Detergent-free purification of ABC (ATP-binding-cassette) transporters

Biochemical Journal

Volumn 461, Issue 2, 2014, Pages 269-278

  Gulati, Sonali ^a   Jamshad, Mohammed ^b   Knowles, Timothy J ^b   Morrison, Kerrie A ^a   Downing, Rebecca ^a   Cant, Natasha ^c   Collins, Richard ^c   Koenderink, Jan B ^d   Ford, Robert C ^c   Overduin, Michael ^b   Kerr, Ian D ^e   Dafforn, Timothy R ^b   Rothnie, Alice J ^a  

^a ASTON UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

^d RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^e UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Detergent; Membrane protein; Nanodisc; Polymer; Purification; Solubilization

Indexed keywords

ABC TRANSPORTER; BREAST CANCER RESISTANCE PROTEIN; COPOLYMER; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; MEMBRANE PROTEIN; MULTIDRUG RESISTANCE ASSOCIATED PROTEIN 1; MULTIDRUG RESISTANCE ASSOCIATED PROTEIN 4; MULTIDRUG RESISTANCE PROTEIN; POLYMER; POLYSTYRENE CO MALEIC ACID; SMA LIPID PARTICLE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ENCAPSULATION; GENE EXPRESSION SYSTEM; INSECT CELL; LIGAND BINDING; LIPID BILAYER; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN STABILITY; SOLUBILIZATION; THERMOSTABILITY; ULTRACENTRIFUGATION;

ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; CLONING, MOLECULAR; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; HUMANS; KINETICS; LIGANDS; MALEATES; MICE; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; NEOPLASM PROTEINS; P-GLYCOPROTEIN; POLYSTYRENES; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STABILITY; RECOMBINANT PROTEINS;

EID: 84903291490     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20131477     Document Type: Article

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