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Volumn 31, Issue 4, 2014, Pages 115-125

Hygromycin-resistance vectors for gene expression in Pichia pastoris

Author keywords

Hygromycin resistance; Pichia pastoris; Protein expression; Selectable markers; Transformation

Indexed keywords

ANTIBIOTIC G 418; GLUTATHIONE; GREEN FLUORESCENT PROTEIN; HUMAN SERUM ALBUMIN; HYGROMYCIN; PHLEOMYCIN; RECOMBINANT PROTEIN; S ADENOSYLMETHIONINE; TRANSCRIPTION ELONGATION FACTOR;

EID: 84897501206     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.3001     Document Type: Article
Times cited : (19)

References (39)
  • 1
    • 84862302788 scopus 로고    scopus 로고
    • Construction of new Pichia pastoris X-33 strains for production of lycopene and β-carotene
    • Araya-Garay JM, Feijoo-Siota L, Rosa-dos-Santos F, et al. 2012. Construction of new Pichia pastoris X-33 strains for production of lycopene and β-carotene. Appl Microbiol Biotechnol 93: 2483-2492.
    • (2012) Appl Microbiol Biotechnol , vol.93 , pp. 2483-2492
    • Araya-Garay, J.M.1    Feijoo-Siota, L.2    Rosa-dos-Santos, F.3
  • 2
    • 84859199206 scopus 로고    scopus 로고
    • Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in silico evaluation of their potentials
    • Caspeta L, Shoaie S, Agren R, et al. 2012. Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in silico evaluation of their potentials. BMC Syst Biol 6: 24.
    • (2012) BMC Syst Biol , vol.6 , pp. 24
    • Caspeta, L.1    Shoaie, S.2    Agren, R.3
  • 3
    • 84863106555 scopus 로고    scopus 로고
    • Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production
    • Chen MT, Lin S, Shandil I, et al. 2012. Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production. Microb Cell Fact 11: 91.
    • (2012) Microb Cell Fact , vol.11 , pp. 91
    • Chen, M.T.1    Lin, S.2    Shandil, I.3
  • 4
    • 77954040575 scopus 로고    scopus 로고
    • Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement
    • Chung BK, Selvarasu S, Andrea C, et al. 2010. Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement. Microb Cell Fact 9: 50.
    • (2010) Microb Cell Fact , vol.9 , pp. 50
    • Chung, B.K.1    Selvarasu, S.2    Andrea, C.3
  • 5
    • 0022395208 scopus 로고
    • Pichia pastoris as a host system for transformations
    • Cregg JM, Barringer KJ, Hessler AY, et al. 1985. Pichia pastoris as a host system for transformations. Mol Cell Biol 5: 3376-3385.
    • (1985) Mol Cell Biol , vol.5 , pp. 3376-3385
    • Cregg, J.M.1    Barringer, K.J.2    Hessler, A.Y.3
  • 7
    • 0025376034 scopus 로고
    • Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance
    • Drocourt D, Calmels T, Reynes JP, et al. 1990. Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucleic Acids Res 18: 4009.
    • (1990) Nucleic Acids Res , vol.18 , pp. 4009
    • Drocourt, D.1    Calmels, T.2    Reynes, J.P.3
  • 8
    • 33748809259 scopus 로고    scopus 로고
    • The budding yeast Pichia pastoris has a novel Sec23p homolog
    • Esaki M, Liu Y, Glick BS. 2006. The budding yeast Pichia pastoris has a novel Sec23p homolog. FEBS Lett 580: 5215-5221.
    • (2006) FEBS Lett , vol.580 , pp. 5215-5221
    • Esaki, M.1    Liu, Y.2    Glick, B.S.3
  • 9
    • 0031610417 scopus 로고    scopus 로고
    • Use of Pichia pastoris as a model eukaryotic system. Peroxisome biogenesis
    • Faber KN, Elgersma Y, Heyman JA, et al. 1998. Use of Pichia pastoris as a model eukaryotic system. Peroxisome biogenesis. Methods Mol Biol 103: 121-147.
    • (1998) Methods Mol Biol , vol.103 , pp. 121-147
    • Faber, K.N.1    Elgersma, Y.2    Heyman, J.A.3
  • 10
    • 69249245323 scopus 로고    scopus 로고
    • Improved glutathione production by gene expression in Pichia pastoris
    • Fei L, Wang Y, Chen S. 2009. Improved glutathione production by gene expression in Pichia pastoris. Bioprocess Biosyst Eng 32: 729-735.
    • (2009) Bioprocess Biosyst Eng , vol.32 , pp. 729-735
    • Fei, L.1    Wang, Y.2    Chen, S.3
  • 11
    • 78049451375 scopus 로고    scopus 로고
    • Engineering of biotin-prototrophy in Pichia pastoris for robust production processes
    • Gasser B, Dragosits M, Mattanovich D. 2010. Engineering of biotin-prototrophy in Pichia pastoris for robust production processes. Metab Eng 12: 573-580.
    • (2010) Metab Eng , vol.12 , pp. 573-580
    • Gasser, B.1    Dragosits, M.2    Mattanovich, D.3
  • 12
    • 0032873415 scopus 로고    scopus 로고
    • Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae
    • Goldstein AL, McCusker JH. 1999. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15: 1541-1553.
    • (1999) Yeast , vol.15 , pp. 1541-1553
    • Goldstein, A.L.1    McCusker, J.H.2
  • 13
    • 0029994841 scopus 로고    scopus 로고
    • A new efficient gene disruption cassette for repeated use in budding yeast
    • Guldener U, Heck S, Fielder T, et al. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24: 2519-2524.
    • (1996) Nucleic Acids Res , vol.24 , pp. 2519-2524
    • Guldener, U.1    Heck, S.2    Fielder, T.3
  • 14
    • 84860649085 scopus 로고    scopus 로고
    • Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures
    • Jorda J, Jouhten P, Camara E, et al. 2012. Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures. Microb Cell Fact 11: 57.
    • (2012) Microb Cell Fact , vol.11 , pp. 57
    • Jorda, J.1    Jouhten, P.2    Camara, E.3
  • 15
    • 0027979938 scopus 로고
    • Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae
    • Kimura M, Kamakura T, Tao QZ, et al. 1994. Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae. Mol Gen Genet 242: 121-129.
    • (1994) Mol Gen Genet , vol.242 , pp. 121-129
    • Kimura, M.1    Kamakura, T.2    Tao, Q.Z.3
  • 16
    • 0036669602 scopus 로고    scopus 로고
    • Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris
    • Lin-Cereghino GP, Lin-Cereghino J, Ilgen C, et al. 2002. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 13: 329-332.
    • (2002) Curr Opin Biotechnol , vol.13 , pp. 329-332
    • Lin-Cereghino, G.P.1    Lin-Cereghino, J.2    Ilgen, C.3
  • 17
    • 0033955337 scopus 로고    scopus 로고
    • Heterologous protein expression in the methylotrophic yeast Pichia pastoris
    • Lin-Cereghino J, Cregg JM. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24: 45-66.
    • (2000) FEMS Microbiol Rev , vol.24 , pp. 45-66
    • Lin-Cereghino, J.1    Cregg, J.M.2
  • 18
    • 42549165096 scopus 로고    scopus 로고
    • Direct selection of Pichia pastoris expression strains using new G418 resistance vectors
    • Lin-Cereghino J, Hashimoto MD, Moy A, et al. 2008. Direct selection of Pichia pastoris expression strains using new G418 resistance vectors. Yeast 25: 293-299.
    • (2008) Yeast , vol.25 , pp. 293-299
    • Lin-Cereghino, J.1    Hashimoto, M.D.2    Moy, A.3
  • 19
    • 17244363998 scopus 로고    scopus 로고
    • Heterologous protein production using the Pichia pastoris expression system
    • Macauley-Patrick S, Fazenda ML, McNeil B, et al. 2005. Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270.
    • (2005) Yeast , vol.22 , pp. 249-270
    • Macauley-Patrick, S.1    Fazenda, M.L.2    McNeil, B.3
  • 20
    • 20244370528 scopus 로고    scopus 로고
    • Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris
    • Mansur M, Cabello C, Hernandez L, et al. 2005. Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris. Biotechnol Lett 27: 339-345.
    • (2005) Biotechnol Lett , vol.27 , pp. 339-345
    • Mansur, M.1    Cabello, C.2    Hernandez, L.3
  • 21
    • 49949115796 scopus 로고    scopus 로고
    • Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris
    • Marx H, Mattanovich D, Sauer M. 2008. Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris. Microb Cell Fact 7: 23.
    • (2008) Microb Cell Fact , vol.7 , pp. 23
    • Marx, H.1    Mattanovich, D.2    Sauer, M.3
  • 22
    • 0344466719 scopus 로고    scopus 로고
    • Cloning and disruption of the PpURA5 gene and construction of a set of integration vectors for the stable genetic modification of Pichia pastoris
    • Nett JH, Gerngross TU. 2003. Cloning and disruption of the PpURA5 gene and construction of a set of integration vectors for the stable genetic modification of Pichia pastoris. Yeast 20: 1279-1290.
    • (2003) Yeast , vol.20 , pp. 1279-1290
    • Nett, J.H.1    Gerngross, T.U.2
  • 23
    • 17144386659 scopus 로고    scopus 로고
    • Cloning and disruption of the Pichia pastoris ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and their use as auxotrophic markers
    • Nett JH, Hodel N, Rausch S, et al. 2005. Cloning and disruption of the Pichia pastoris ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and their use as auxotrophic markers. Yeast 22: 295-304.
    • (2005) Yeast , vol.22 , pp. 295-304
    • Nett, J.H.1    Hodel, N.2    Rausch, S.3
  • 24
    • 0031916703 scopus 로고    scopus 로고
    • Analysis of Pichia pastoris components in recombinant human serum albumin by immunological assays and by HPLC with pulsed amperometric detection
    • Ohtani W, Ohda T, Sumi A, et al. 1998. Analysis of Pichia pastoris components in recombinant human serum albumin by immunological assays and by HPLC with pulsed amperometric detection. Anal Chem 70: 425-429.
    • (1998) Anal Chem , vol.70 , pp. 425-429
    • Ohtani, W.1    Ohda, T.2    Sumi, A.3
  • 25
    • 67649993654 scopus 로고    scopus 로고
    • Expression of GFP using Pichia pastoris vectors with zeocin or G-418 sulphate as the primary selectable marker
    • Papakonstantinou T, Harris S, Hearn MT. 2009. Expression of GFP using Pichia pastoris vectors with zeocin or G-418 sulphate as the primary selectable marker. Yeast 26: 311-321.
    • (2009) Yeast , vol.26 , pp. 311-321
    • Papakonstantinou, T.1    Harris, S.2    Hearn, M.T.3
  • 26
    • 14744304041 scopus 로고
    • Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression
    • Scorer CA, Clare JJ, McCombie WR, et al. 1994. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Biotechnology (NY) 12: 181-184.
    • (1994) Biotechnology (NY) , vol.12 , pp. 181-184
    • Scorer, C.A.1    Clare, J.J.2    McCombie, W.R.3
  • 27
    • 2342478416 scopus 로고    scopus 로고
    • The transitional ER localization mechanism of Pichia pastoris Sec12
    • Soderholm J, Bhattacharyya D, Strongin D, et al. 2004. The transitional ER localization mechanism of Pichia pastoris Sec12. Dev Cell 6: 649-659.
    • (2004) Dev Cell , vol.6 , pp. 649-659
    • Soderholm, J.1    Bhattacharyya, D.2    Strongin, D.3
  • 28
    • 77954743150 scopus 로고    scopus 로고
    • Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production
    • Sohn SB, Graf AB, Kim TY, et al. 2010. Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production. Biotechnol J 5: 705-715.
    • (2010) Biotechnol J , vol.5 , pp. 705-715
    • Sohn, S.B.1    Graf, A.B.2    Kim, T.Y.3
  • 29
    • 33846268057 scopus 로고    scopus 로고
    • Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates
    • Solà A, Jouhten P, Maaheimo H, et al. 2007. Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates. Microbiology 153: 281-290.
    • (2007) Microbiology , vol.153 , pp. 281-290
    • Solà, A.1    Jouhten, P.2    Maaheimo, H.3
  • 30
    • 3042652967 scopus 로고    scopus 로고
    • Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris
    • Solà A, Maaheimo H, Ylönen K, et al. 2004. Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris. Eur J Biochem 271: 2462-2470.
    • (2004) Eur J Biochem , vol.271 , pp. 2462-2470
    • Solà, A.1    Maaheimo, H.2    Ylönen, K.3
  • 31
    • 1842839988 scopus 로고    scopus 로고
    • The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris
    • Sunga AJ, Cregg JM. 2004. The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris. Gene 330: 39-47.
    • (2004) Gene , vol.330 , pp. 39-47
    • Sunga, A.J.1    Cregg, J.M.2
  • 32
    • 49749139770 scopus 로고    scopus 로고
    • Posttransformational vector amplification in the yeast Pichia pastoris
    • Sunga AJ, Tolstorukov I, Cregg JM. 2008. Posttransformational vector amplification in the yeast Pichia pastoris. FEMS Yeast Res 8: 870-876.
    • (2008) FEMS Yeast Res , vol.8 , pp. 870-876
    • Sunga, A.J.1    Tolstorukov, I.2    Cregg, J.M.3
  • 33
    • 0031457095 scopus 로고    scopus 로고
    • Metabolism of sulfur amino acids in Saccharomyces cerevisiae
    • Thomas D, Surdin-Kerjan Y. 1997. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 61: 503-532.
    • (1997) Microbiol Mol Biol Rev , vol.61 , pp. 503-532
    • Thomas, D.1    Surdin-Kerjan, Y.2
  • 34
    • 21644484985 scopus 로고    scopus 로고
    • Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker
    • Thor D, Xiong S, Orazem CC, et al. 2005. Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker. FEMS Yeast Res 5: 935-942.
    • (2005) FEMS Yeast Res , vol.5 , pp. 935-942
    • Thor, D.1    Xiong, S.2    Orazem, C.C.3
  • 35
    • 68449103617 scopus 로고    scopus 로고
    • Stabilized gene duplication enables long-term selection-free heterologous pathway expression
    • Tyo KE, Ajikumar PK, Stephanopoulos G. 2009. Stabilized gene duplication enables long-term selection-free heterologous pathway expression. Nat Biotechnol 27: 760-765.
    • (2009) Nat Biotechnol , vol.27 , pp. 760-765
    • Tyo, K.E.1    Ajikumar, P.K.2    Stephanopoulos, G.3
  • 36
    • 84862793157 scopus 로고    scopus 로고
    • Enhanced co-production of S-adenosylmethionine and glutathione by an ATP-oriented amino acid addition strategy
    • Wang Y, Wang D, Wei G, et al. 2012. Enhanced co-production of S-adenosylmethionine and glutathione by an ATP-oriented amino acid addition strategy. Bioresour Technol 107: 19-24.
    • (2012) Bioresour Technol , vol.107 , pp. 19-24
    • Wang, Y.1    Wang, D.2    Wei, G.3
  • 37
    • 65549168275 scopus 로고    scopus 로고
    • mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris
    • Yang J, Jiang W, Yang S. 2009. mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMS Yeast Res 9: 600-609.
    • (2009) FEMS Yeast Res , vol.9 , pp. 600-609
    • Yang, J.1    Jiang, W.2    Yang, S.3
  • 38
    • 0037261058 scopus 로고    scopus 로고
    • Enhancement of the production of SAM by overexpression of SAM synthetase in Pichia pastoris
    • Yu ZL, Wu XJ, Li DY, et al. 2003. Enhancement of the production of SAM by overexpression of SAM synthetase in Pichia pastoris. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35: 127-132.
    • (2003) Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) , vol.35 , pp. 127-132
    • Yu, Z.L.1    Wu, X.J.2    Li, D.Y.3
  • 39
    • 42549143546 scopus 로고    scopus 로고
    • Disruption of Pichia pastoris PMR1 gene decreases its folding capacity on human serum albumin and interferon-α2b fusion protein
    • Zhao HL, Xue C, Wang Y, et al. 2008. Disruption of Pichia pastoris PMR1 gene decreases its folding capacity on human serum albumin and interferon-α2b fusion protein. Yeast 25: 279-286.
    • (2008) Yeast , vol.25 , pp. 279-286
    • Zhao, H.L.1    Xue, C.2    Wang, Y.3


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