Posttransformational vector amplification in the yeast Pichia pastoris

FEMS Yeast Research

Volumn 8, Issue 6, 2008, Pages 870-876

  Sunga, Anthony Jay ^a   Tolstorukov, Ilya ^a   Cregg, James M ^a  

^a KECK GRADUATE INSTITUTE   (United States)

Author keywords

Amplification; Multicopy strains; Pichia pastoris; Transformation; Yeast

Indexed keywords

RECOMBINANT PROTEIN;

ARTICLE; CLONING VECTOR; CONTROLLED STUDY; DNA EXTRACTION; GENE AMPLIFICATION; GENE SEQUENCE; GENOME; NONHUMAN; PICHIA PASTORIS; SCREENING; YEAST;

BETA-LACTAMASES; BLEOMYCIN; DRUG RESISTANCE, FUNGAL; ELECTROPORATION; FUNGAL PROTEINS; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; GENETIC ENGINEERING; GENETIC MARKERS; GENETIC VECTORS; PICHIA; SELECTION (GENETICS); TRANSFORMATION, GENETIC;

PICHIA PASTORIS;

EID: 49749139770     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/j.1567-1364.2008.00410.x     Document Type: Article

References (30)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA Struhl K (eds) (2001a) Southern Blotting. Current Protocols in Molecular Biology. John Wiley and Sons Inc., New York. 1: 2.1 2.9.
2. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA Struhl K (eds) (2001b) Enzymatic manipulation of DNA and RNA. Current Protocols in Molecular Biology. John Wiley & Sons Inc., New York. 1: 3.1 3.19.
3. Brierley RA (1998) Secretion of recombinant human insulin-like growth factor I (IGF-I). Methods Mol Biol 103: 149 177.
4. Cereghino GP Cregg JM (1999) Applications of yeast in biotechnology: protein production and genetic analysis. Curr Opin Biotechnol 10: 422 427.
5. Cereghino GP, Cereghino JL, Ilgen C Cregg JM (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 13: 329 332.
6. Cereghino JL Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24: 45 66.
7. Clare JJ, Rayment FB, Ballantine SP, Sreekrishna K Romanos MA (1991) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Biotechnology (NY) 9: 455 460.
8. Cregg JM (ed) (2007) DNA-mediated transformation. Pichia Protocols: Methods in Molecular Biology, Vol. 389, pp. 27 42. Humana Press, Totowa, NJ.
9. Cregg JM Russell KA (1998) Transformation. Methods Mol Biol 103: 27 39.
10. Cregg JM, Barringer KJ, Hessler AY Madden KR (1985) Pichia pastoris as a host system for transformations. Mol Cell Biol 5: 3376 3385.
11. Cregg JM, Madden KR, Barringer KJ, Thill GP Stillman CA (1989) Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol Cell Biol 9: 1316 1323.
12. Cregg JM, Shen S, Johnson M Waterham HR (1998) Classical genetic manipulation. Methods Mol Biol 103: 17 26.
13. Dorsey M, Peterson C, Bray K Paquin CE (1992) Spontaneous amplification of the ADH4 gene in Saccharomyces cerevisiae. Genetics 132: 943 950.
14. Ellis SB, Brust PF, Koutz PJ, Waters AF, Harpold MM Gingeras TR (1985) Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol Cell Biol 5: 1111 1121.
15. Fogel S Welch JW (1982) Tandem gene amplification mediates copper resistance in yeast. Proc Natl Acad Sci USA 79: 5342 5346.
16. Gellissen G (ed) (2004) Yeast systems. Production of Recombinant Proteins: Microbial and Eukaryotic Expression Systems, pp. 111 185. Wiley-VCH Verlag, Weinheim, Germany.
17. Ilgen C, Cereghino JL Cregg JM (2004) Pichia pastoris. Production of Recombinant Proteins: Microbial and Eukaryotic Expression Systems (Gellissen G, ed), pp. 143 162. Wiley-VCH Verlag, Weinheim, Germany.
18. Kramer MF Coen DM (2000) The polymerase chain reaction. Current Protocols in Molecular Biology (Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA Struhl K, eds), pp. 15.0.3 15.1.14. John Wiley and Sons Inc., New York.
19. Mansur M, Cabello C, Hernandez L et al 2005) Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris. Biotechnol Lett 27: 339 345.
20. Orr-Weaver TL Szostak JW (1983) Multiple, tandem plasmid integration in Saccharomyces cerevisiae. Mol Cell Biol 3: 747 749.
21. Orr-Weaver TL, Szostak JW Rothstein RJ (1981) Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci USA 78: 6354 6358.
22. Plessis A Dujon B (1993) Multiple tandem integrations of transforming DNA sequences in yeast chromosomes suggest a mechanism for integrative transformation by homologous recombination. Gene 134: 41 50.
23. Romanos M, Scorer C, Sreekrishna K Clare J (1998) The generation of multicopy recombinant strains. Methods Mol Biol 103: 55 72.
24. Saffran WA, Smith ED Chan SK (1991) Induction of multiple plasmid recombination in Saccharomyces cerevisiae by psoralen reaction and double strand breaks. Nucleic Acids Res 19: 5681 5687.
25. Schimke RT (1986) Methotrexate resistance and gene amplification. Mechanisms and implications. Cancer 57: 1912 1917.
26. Shen S, Sulter G, Jeffries TW Cregg JM (1998) A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris. Gene 216: 93 102.
27. Stark GR Wahl GM (1984) Gene amplification. Annu Rev Biochem 53: 447 491.
28. Sunga AJ Cregg JM (2004) The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris. Gene 330: 39 47.
29. Vassileva A, Chugh DA, Swaminathan S Khanna N (2001) Effect of copy number on the expression levels of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. Protein Expr Purif 21: 71 80.
30. Waterham HR, Russell KA, Vries Y Cregg JM (1997) Peroxisomal targeting, import, and assembly of alcohol oxidase in Pichia pastoris. J Cell Biol 139: 1419 1431.