Expression of GFP using Pichia pastoris vectors with zeocin or G-418 sulphate as the primary selectable marker

Yeast

Volumn 26, Issue 6, 2009, Pages 311-321

  Papakonstantinou, Theo ^a   Harris, Simon ^a   Hearn, Milton T W ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Green fluorescent protein; Immobilized metal ion affinity; Kanamycin; Pichia pastoris; Protein expression; Selectable markers

Indexed keywords

ANTIBIOTIC G 418; GREEN FLUORESCENT PROTEIN; HEXAHISTIDINE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; ZEOCIN; ANTIINFECTIVE AGENT; ANTINEOPLASTIC ANTIBIOTIC; BLEOMYCIN; GENTAMICIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM TRANSFORMATION; CELL LYSATE; CONTROLLED STUDY; EXPRESSION VECTOR; FLUORESCENCE; FUNGAL STRAIN; GENE CONSTRUCT; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC STABILITY; HETEROLOGOUS EXPRESSION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; PICHIA PASTORIS; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; YEAST CELL; ANTIFUNGAL RESISTANCE; BIOSYNTHESIS; DRUG EFFECT; GENE VECTOR; GENETIC SELECTION; GENETIC TRANSFORMATION; GENETICS; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MOLECULAR CLONING; PICHIA;

PICHIA PASTORIS;

ANTI-INFECTIVE AGENTS; ANTIBIOTICS, ANTINEOPLASTIC; BLEOMYCIN; CLONING, MOLECULAR; DRUG RESISTANCE, FUNGAL; GENETIC ENGINEERING; GENETIC VECTORS; GENTAMICINS; GREEN FLUORESCENT PROTEINS; MICROBIAL SENSITIVITY TESTS; PICHIA; RECOMBINANT PROTEINS; SELECTION (GENETICS); TRANSFORMATION, GENETIC;

EID: 67649993654     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1666     Document Type: Article

References (56)

1. Barth C, Fraser DJ, Fisher PR. 1998. Co-insertional replication is responsible for tandem multimer formation during plasmid integration into the Dictyostelium genome. Plasmid 39: 141-153.
2. Berman AE, Kozlova NI, Morozevich GE. 2003. Integrins: structure and signaling. Biochemistry (Mosc) 68: 1284-1299.
3. Boer E, Steinborn G, Kunze G, Gellissen G. 2007. Yeast expression platforms. Appl Microbiol Biotechnol 77: 513-523.
4. Boettner M, Prinz B, Holz C, et al. 2002. High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris. J Biotechnol 99: 51-62.
5. Campbell TN, Choy FY. 2002. Expression of two green fluorescent protein variants in citrate-buffered media in Pichia pastoris. Anal Biochem 311: 193-195.
6. Chalfie M, Tu Y, Euskirchen G, et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
7. Clare JJ, Romanos MA, Rayment FB, et al. 1991. Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105: 205-212.
8. Crane DI, Gould SJ. 1994. The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a non-reverting his4 deletion mutant, and development of HIS4-based replicating and integrating plasmids. Curr Genet 26: 443-450.
9. Cregg JM, Barringer KJ, Hessler AY, Madden KR. 1985. Pichia pastoris as a host system for transformations. Mol Cell Biol 5: 3376-3385.
10. Cregg JM, Madden KR, Barringer KJ, et al. 1989. Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol Cell Biol 9: 1316-1323.
11. Cregg JM, Tschopp JF, Stillmann C, et al. 1987. High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. BioTechnology 5: 479-485.
12. Cregg JM, Vedvick TS, Raschke WC. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. BioTechnology 11: 905-910.
13. Daly R, Hearn MTW. 2005. Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J Mol Recognit 18: 119-138.
14. Early AE, Williams JG. 1987. Two vectors which facilitate gene manipulation and a simplified transformation procedure for Dictyostelium discoideum. Gene 59: 99-106.
15. Eckart MR, Bussineau CM. 1996. Quality and authenticity of heterologous proteins synthesized in yeast. Curr Opin Biotechnol 7: 525-530.
16. Eiden-Plach A, Zagorc T, Heintel T, et al. 2004. Viral preprotoxin signal seqence allows efficient secretion of green fluorescent protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe. Appl Microbiol Biotechnol 70: 961-966.
17. Ellis SB, Brust PF, Koutz PJ, et al. 1985. Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol Cell Biol 5: 1111-1121.
18. Gaudet P, Pilcher KE, Fey P, Chisholm RL. 2007. Transformation of Dictyostelium discoideum with plasmid DNA. Nat Protocols 2: 1317-1324.
19. Gellissen G. 2000. Heterologous protein production in methylotrophic yeasts. Appl Microbiol Biotechnol 54: 741-750.
20. Hamilton SR, Gerngross TU. 2007. Glycosylation engineering in yeast: the advent of fully humanized yeast. Curr Opin Biotechnol 18: 387-392.
21. Higgins DR, Busser K, Comiskey J, et al. 1998. Small vectors for expression based on dominant drug resistance with direct multicopy selection. Methods Mol Biol 103: 41-53.
22. Hisiger S, Jolicoeur M. 2005. A multiwavelength fluorescence probe: is one probe capable for on-line monitoring of recombinant protein production and biomass activity? J Biotechnol 117: 325-336.
23. Ilgen C, Lin Cereghino J, Cregg JM. 2005. Pichia pastoris. In Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems, Gellisen G (ed.). Wiley-VCH: Weinheim; 143-162.
24. Invitrogen Corporation: http://www.invitrogen.com (accessed: October 5, 2008).
25. Jahic M, Veide A, Charoenrat T, et al. 2006. Process technology for production and recovery of heterologous proteins with Pichia pastoris. Biotechnol Prog 22: 1465-1473.
26. Kain SR, Adams M, Kondepudi A, et al. 1995. Green fluorescent protein as a reporter of gene expression and protein localization. Biotechniques 19: 650-655.
27. Keenan PO, Knight AW, Billinton N, et al. 2007. Clear and present danger? The use of a yeast biosensor to monitor changes in the toxicity of industrial effluents subjected to oxidative colour removal treatments. J Environ Monit 9: 1394-1401.
28. Lenassi Zupan A, Trobec S, Gaberc-Porekar V, Menart V. 2004. High expression of green fluorescent protein in Pichia pastoris leads to formation of fluorescent particles. J Biotechnol 109: 115-122.
29. Lin Cereghino GP, Lin Cereghino J, Sunga AJ, et al. 2001. New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Gene 263: 159-169.
30. Lin Cereghino J, Cregg JM. 2000. Heterologous protein expression in the methylotropic yeast Pichia pastoris. FEMS Microbiol Rev 24: 45-66.
31. Lin Cereghino J, Hashimoto MD, Moy A, et al. 2008. Direct selection of Pichia pastoris expression strains using new G418 resistance vectors. Yeast 25: 293-299.
32. Lin Cereghino J, Lin Cereghino GP. 2007. Vectors and strains for expression. Methods Mol Biol 389: 11-26.
33. Liu H, Tan X, Russell KA, et al. 1995. PER3, a gene required for peroxisome biogenesis in Pichia pastoris, encodes a peroxisomal membrane protein involved in protein import. J Biol Chem 270: 10940-10951.
34. Liu MQ, Liu GF. 2008. Expression of recombinant Bacillus licheniformis xylanase A in Pichia pastoris and xylooligosaccharides released from xylans by it. Protein Expr Purif 57: 101-107.
35. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM. 2005. Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270.
36. Miles D, Busser K, Stadler C, Higgins D. 1998. Isolation of nucleic acids. In Pichia Protocols, Higgins D, Cregg JM (eds). Humana: Totowa, NJ; 73-80.
37. Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM. 1999. Aminoglycosides: activity and resistance. Antimicrob Agents Chemother 43: 727-737.
38. Nett JH, Gerngross TU. 2003. Cloning and disruption of the PpURA5 gene and construction of a set of integration vectors for the stable genetic modification of Pichia pastoris. Yeast 20: 1279-1290.
39. Nett JH, Hodel N, Rausch S, Wildt S. 2005. Cloning and disruption of the Pichia pastoris ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and their use as auxotrophic markers. Yeast 22: 295-304.
40. Pang KM, Lynes MA, Knecht DA. 1999. Variables controlling the expression level of exogenous genes in Dictyostelium. Plasmid 41: 187-197.
41. Papakonstantinou T, Harris SJ, Fredericks D, et al. 2009. Synthesis, purification and bioactivity of recombinant human activin A expressed in the yeast Pichia pastoris. Prot Express Purif 64: 131-138.
42. Resina D, Bollók M, Khatri NK, et al. 2007. Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction. Microb Cell Fact 6: 21.
43. Resina D, Cos O, Ferrer P, Valero F. 2005. Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter. Biotechnol Bioeng 91: 760-767.
44. Romanos MA, Scorer CA, Clare JJ. 1992. Foreign gene expression in yeast: a review. Yeast 8: 423-488.
45. Sambrook J, Russell DWJ. 2001. Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY.
46. Scorer CA, Clare JJ, McCombie WR, et al. 1994. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. BioTechnology 12: 181-184.
47. Sears IB, O'Connor J, Rossanese OW, Glick BS. 1998. A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris. Yeast 14: 783-790.
48. Shen S, Sulter G, Jeffries TW, Cregg JM. 1998. A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris. Gene 216: 93-102.
49. Sreekrishna K, Potenz RH, Cruze JA, et al. 1988. High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris. J Basic Microb 28: 265-278.
50. Sunga AJ, Cregg JM. 2004. The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris. Gene 330: 39-47.
51. Swain M, Ross GW. 1995. A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels. Electrophoresis 16: 948-951.
52. Thor D, Xiong S, Orazem CC, et al. 2005. Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker. FEMS Yeast Res 5: 935-942.
53. Tschopp JF, Brust PF, Cregg JM, et al. 1987. Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res 15: 3859-3876.
54. Wach A. 1996. PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12: 259-265.
55. Wan H, Sjölinder M, Schairer HU, Leclerque A. 2004. A new dominant selection marker for transformation of Pichia pastoris to soraphen A resistance. J Microbiol Methods 57: 33-39.
56. Waterham HR, Digan ME, Koutz PJ, et al. 1997. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene 186: 37-44.