The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris

Gene

Volumn 330, Issue 1-2, 2004, Pages 39-47

  Sunga, Anthony Jay ^a   Cregg, James M ^a  

^a KECK GRADUATE INSTITUTE   (United States)

Author keywords

Alcohol oxidase protein; Amp; AOX1; AOX2; Aoxp; Escherichia coli gene encoding lactamase; Expression vectors; Methylotrophic yeast; Pichia pastoris gene encoding alcohol oxidase I; Recombinant protein production

Indexed keywords

BIOLOGICAL MARKER; CARBON; FORMALDEHYDE; FORMALDEHYDE DEHYDROGENASE; FORMIC ACID; GENE PRODUCT; METHANOL; METHYLAMINE; NITROGEN;

ANTIBIOTIC RESISTANCE; ARTICLE; EXPRESSION VECTOR; FUNGAL STRAIN; FUNGUS GROWTH; GENE EXPRESSION; GENE REPLICATION; HOST; NONHUMAN; OXIDATION; PICHIA PASTORIS; PRIORITY JOURNAL; RECOMBINANT GENE;

ALDEHYDE OXIDOREDUCTASES; GENE DOSAGE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, FUNGAL; GENETIC MARKERS; GENETIC VECTORS; MUTATION; PICHIA; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; OTHER SEQUENCES; PICHIA; PICHIA PASTORIS; VECTORS;

EID: 1842839988     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2003.12.015     Document Type: Article

References (32)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Smith, J.A., Seidman, J.G., Struhl, K., 2000. Current Protocols in Molecular Biology, Wiley, New York, pp. 2.9.1-2.9.15, 13.11.1-13.11.4.
2. Brierley R.A. Secretion of recombinant human insulin-like growth factor I (IGF-I). Higgins D.R., Cregg J.M. Methods in Molecular Biology: Pichia Protocols. 1998;149-177 Humana Press, Totowa, NJ.
3. Clare J.J., Rayment F.B., Ballantine S.P., Sreekrishna K., Romanos M.A. High-level expression of tetanus toxin fragment c in Pichia pastoris strains containing multiple tandem integrations of the gene. Bio/Technology. 9:1991;455-460.
4. Cregg J.M., Russell K.A. Transformation. Higgins D.R., Cregg J.M. Methods in Molecular Biology: Pichia Protocols. 1998;27-39 Humana Press, Totowa, NJ.
5. Cregg J.M., Barringer K.J., Hessler A.Y., Madden K.R. Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5:1985;3376-3385.
6. Cregg J.M., Tschoop J.F., Stillman C., Siegel R., Akong M., Craig W.S., Buckholz R.G., Madden K.R., Kellaris P.A., Davis G.R., Smiley B.L., Cruze J., Torregrossa R., Velicelebi G., Thill G.P. High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. Bio/Technology. 5:1987;479-485.
7. Cregg J.M., Digan M.E., Tschopp R.A., Brierley W.S., Craig G., Velicelebi R.S., Thill G.P. Expression of foreign genes in Pichia pastoris. Hershberger C.L., Queener S.W., Hegeman G. Genetics and Molecular Biology of Industrial Microorganisms. 1989;343-352 American Society for Microbiology, Washington, DC.
8. Cregg J.M., Madden K.R., Barringer K.J., Thill G.P., Stillman C.A. Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol. Cell. Biol. 9:1989;1316-1323.
9. Ellis S.B., Brust P.F., Koutz P.J., Water A.F., Harpold M.M., Gingeras T.R. Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol. Cell. Biol. 5:1985;1111-1121.
10. Higgins D.R., Cregg J.M. Introduction to Pichia pastoris. Higgins D.R., Cregg J.M. Methods in Molecular Biology: Pichia Protocols. 1998;1-15 Humana Press, Totowa, NJ.
11. Higgins D.R., Busser K., Comiskey J., Whittier P.S., Purcell T.J., Hoeffler J.P. Small vector for expression based on dominant drug resistance with direct multicopy selection. Higgins D.R., Cregg J.M. Methods in Molecular Biology: Pichia protocols. 1998;41-53 Humana Press, Totowa, NJ.
12. Kramer M.F., Coen D.M. The polymerase chain reaction. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Current Protocols in Molecular Biology. 2000;15.0.3-15.1.14.
13. Lin Cereghino G.P., Cregg J.M. Applications of yeast in biotechnology: protein production and genetic analysis. Curr. Opin. Biotechnol. 10:1999;422-427.
14. Lin Cereghino J., Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:2000;45-66.
15. Lin Cereghino G.P., Lin Cereghino J., Sunga A.J., Johnson M.A., Lim M., Gleeson M., Cregg J.M. New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Gene. 263:2001;159-169.
16. Lin Cereghino G.P., Sunga A.J., Lin Cereghino J., Cregg J.M. Expression of foreign genes in the yeast Pichia pastoris. Setlow J.K. Genetic Engineering. 2001;157-170.
17. Lin Cereghino G.P., Lin Cereghino J., Ilgen C., Cregg J.M. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13:2002;329-332.
18. Liu H., Tan X., Russell K.A., Veenhuis M., Cregg J.M. PER3, a gene required for peroxisome biogenesis in Pichia pastoris, encodes a peroxisomal membrane protein involved in protein import. J. Biol. Chem. 270:1995;10940- 10951.
19. Miles D.J., Busser K., Stadler C., Higgins D.R. Isolation of nucleic acids. Higgins D.R., Cregg J.M. Methods in Molecular Biology: Pichia protocols. 1998;73-80 Humana Press, Totowa, NJ.
20. Romanos M. Advances in the use of Pichia pastoris for high-level expression. Curr. Opin. Biotechnol. 6:1995;527-533.
21. Romanos M.A., Clare J.J., Beesley K.M., Rayment F.B., Ballantine S.P., Makoff A.J., Dougan G., Fairweather N.F., Charles I.G. Recombinant Bordetella pertussis peractin (P69) from the yeast Pichia pastoris: high-level production and immunological properties. Vaccine. 9:1991;901-906.
22. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning. A Laboratory Manual. 2nd edition:1989;16.66-16.67 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Schutte H., Flossdorf J., Sahm H., Kula M.R. Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii. Eur. J. Biochem. 62:1976;151-160.
24. Scorer C.A., Clare J.J., McCombie W.R., Romanos M.A., Sreekrishna K. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Bio/Technology. 12:1994;181-184.
25. Shen S., Sulter G., Jeffries T.W., Cregg J.M. A strong nitrogen source-related promoter for controlled expression of foreign genes in the yeast Pichia pastoris. Gene. 216:1998;93-102.
26. 2 are non-essential for energy supply of yeast methylotrophic growth. Arch. Microbiol. 154:1990;566-575.
27. Tschopp J.F., Brust P.F., Cregg J.M., Stillman C.A., Gingeras T.R. Expression of the lacz gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acid Res. 15:1987;3859-3876.
28. Veenhuis M., van Dijken J.P., Harder W. The significance of peroxisomes in the metabolism of one-carbon compounds in yeast. Adv. Microb. Physiol. 24:1983;1-82.
29. Waterham H.R., Keizer-Gunnink I., Goodman J.M., Harder W., Veenhuis M. Development of multipurpose peroxisomes in Candida boidinii grown in oleic acid-methanol limited continuous cultures. J. Bacteriol. 174:1992;4057-4063.
30. Waterham H.R., Digan M.E., Koutz P.J., Lair S.V., Cregg J.M. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene. 186:1997;37-44.
31. Zhu H., Shi J., Cregg J.M., Woldegiogis G. Reconstitution of highly expressed human heart muscle carnitine palmitoyltransferase I. Biochem. Biophys. Res. Commun. 239:1987;498-502.
32. Zwart K., Veenhuis M., van Dijken J.P., Harder W. Development of amine oxidase-containing peroxisomes in yeast during growth on glucose in the presence of methylamine as the sole source of nitrogen. Arch. Microbiol. 126:1980;117-126.