Shortcomings in the application of multicolour flow cytometry in lymphocyte subsets enumeration

Scandinavian Journal of Immunology

Volumn 79, Issue 2, 2014, Pages 75-89

  Njemini, R ^a   Onyema, O O ^a   Renmans, W ^b   Bautmans, I ^a   De Waele, M ^b   Mets, T ^a  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^b UNIVERSITY HOSPITAL BRUSSELS   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCEIN; FLUOROCHROME; MEMBRANE ANTIGEN; QUANTUM DOT; FLUORESCENT DYE;

ANTIBODY BLOOD LEVEL; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ANTIGEN EXPRESSION; CELL COUNTING; CONJUGATION; DATA ANALYSIS; FAR RED LIGHT; FLOW CYTOMETER; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; INFORMATION PROCESSING; LIGHT EXPOSURE; LYMPHOCYTE SUBPOPULATION; MULTICOLOR FLOW CYTOMETRY; PRIORITY JOURNAL; REVIEW; T LYMPHOCYTE SUBPOPULATION; LYMPHOCYTE COUNT; METHODOLOGY; STATISTICS;

ANTIBODY SPECIFICITY; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENT DYES; HUMANS; LYMPHOCYTE COUNT; LYMPHOCYTE SUBSETS; QUANTUM DOTS; STATISTICS AS TOPIC;

EID: 84897019040     PISSN: 03009475     EISSN: 13653083     Source Type: Journal    
DOI: 10.1111/sji.12142     Document Type: Review

References (64)

1. Tung JW, Parks DR, Moore WA, Herzenberg LA. New approaches to fluorescence compensation and visualization of FACS data. Clin Immunol 2004;110:277-83.
2. Roederer M. Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry 2001;45:194-205.
3. Johansson U, Macey M. Pitfalls in the use of multicolour flow cytometry in haematology. J Clin Pathol 2011;64:561-3.
4. Ierino FL, Hulett MD, McKenzie IF, Hogarth PM. Mapping epitopes of human Fc gamma RII (CDw32) with monoclonal antibodies and recombinant receptors. J Immunol 1993;150:1794-803.
5. Stein R, Belisle E, Hansen HJ, Goldenberg DM. Epitope specificity of the anti-(B cell lymphoma) monoclonal antibody, LL2. Cancer Immunol Immunother 1993;37:293-8.
6. Deka C, Lehnert BE, Lehnert NM, Jones GM, Sklar LA, Steinkamp JA. Analysis of fluorescence lifetime and quenching of FITC-conjugated antibodies on cells by phase-sensitive flow cytometry. Cytometry 1996;25:271-9.
7. Vermes I, Haanen C, Reutelingsperger C. Flow cytometry of apoptotic cell death. J Immunol Methods 2000;243:167-90.
8. Dolezel J, Greilhuber J, Suda J. Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc 2007;2:2233-44.
9. Vines A, McBean GJ, Blanco-Fernandez A. A flow-cytometric method for continuous measurement of intracellular Ca(2+) concentration. Cytometry A 2010;77:1091-7.
10. Njemini R, Demanet C, Mets T. Aging-related differences in basal heat shock protein 70 levels in lymphocytes are linked to altered frequencies of lymphocyte subsets. Aging Cell 2008;7:498-505.
11. Ibanez-Peral R, Bergquist PL, Walter MR, Gibbs M, Goldys EM, Ferrari B. Potential use of quantum dots in flow cytometry. Int J Mol Sci 2008;9:2622-38.
12. Barroso MM. Quantum dots in cell biology. J Histochem Cytochem 2011;59:237-51.
13. Sugar IP, Gonzalez-Lergier J, Sealfon SC. Improved compensation in flow cytometry by multivariable optimization. Cytometry A 2011;79:356-60.
14. Spidlen J, Leif RC, Moore W, Roederer M, Brinkman RR. Gating-ML: XML-based gating descriptions in flow cytometry. Cytometry A 2008;73A:1151-7.
15. Tung JW, Heydari K, Tirouvanziam R, Sahaf B, Parks DR, Herzenberg LA. Modern flow cytometry: a practical approach. Clin Lab Med 2007;27:453-68.
16. Parks DR, Roederer M, Moore WA. A new "Logicle" display method avoids deceptive effects of logarithmic scaling for low signals and compensated data. Cytometry A 2006;69:541-51.
17. Bayer J, Grunwald D, Lambert C, Mayol JF, Maynadie M. Thematic workshop on fluorescence compensation settings in multicolor flow cytometry. Cytometry B Clin Cytom 2007;72:8-13.
18. Roederer M, De Rosa S, Gerstein R et al. 8 color, 10-parameter flow cytometry to elucidate complex leukocyte heterogeneity. Cytometry 1997;29:328-39.
19. Stelzer GT, Marti G, Hurley A, McCoy P Jr, Lovett EJ, Schwartz A. U.S.-Canadian Consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 1997;30:214-30.
20. Maecker HT, Trotter J. Flow cytometry controls, instrument setup, and the determination of positivity. Cytometry A 2006;69:1037-42.
21. Baumgarth N, Roederer M. A practical approach to multicolor flow cytometry for immunophenotyping. J Immunol Methods 2000;243:77-97.
22. Mittag A, Tarnok A. Basics of standardization and calibration in cytometry-a review. J Biophotonics 2009;2:470-81.
23. Roederer M. Compensation is not dependent on signal intensity or on number of parameters. Cytometry 2001;46:357-9.
24. Wang L, Gaigalas AK, Yan M. Quantitative fluorescence measurements with multicolor flow cytometry. Methods Mol Biol 2011;699:53-65.
25. Stewart CC, Stewart SJ. Four color compensation. Cytometry 1999;38:161-75.
26. Mahnke YD, Roederer M. Optimizing a multicolor immunophenotyping assay. Clin Lab Med 2007;27:469-85.
27. Srivastava P, Sladek TL, Goodman MN, Jacobberger JW. Streptavidin-based quantitative staining of intracellular antigens for flow cytometric analysis. Cytometry 1992;13:711-21.
28. Maecker HT, Frey T, Nomura LE, Trotter J. Selecting fluorochrome conjugates for maximum sensitivity. Cytometry A 2004;62:169-73.
29. Hulspas R, O'Gorman MR, Wood BL, Gratama JW, Sutherland DR. Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry B Clin Cytom 2009;76:355-64.
30. van Vugt MJ, van den Herik-Oudijk IE, van de Winkle JG. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood 1996;88:2358-61.
31. Roederer M, Kantor AB, Parks DR, Herzenberg LA. Cy7PE and Cy7APC: bright new probes for immunofluorescence. Cytometry 1996;24:191-7.
32. Mayeno AN, Hamann KJ, Gleich GJ. Granule-associated flavin adenine dinucleotide (FAD) is responsible for eosinophil autofluorescence. J Leukoc Biol 1992;51:172-5.
33. Aubin JE. Autofluorescence of viable cultured mammalian cells. J Histochem Cytochem 1979;27:36-43.
34. Monici M. Cell and tissue autofluorescence research and diagnostic applications. Biotechnol Annu Rev 2005;11:227-56.
35. Benson RC, Meyer RA, Zaruba ME, McKhann GM. Cellular autofluorescence-is it due to flavins? J Histochem Cytochem 1979;27:44-8.
36. Levitt JM, Baldwin A, Papadakis A et al. Intrinsic fluorescence and redox changes associated with apoptosis of primary human epithelial cells. J Biomed Opt 2006;11:064012.
37. Neumann M, Gabel D. Simple method for reduction of autofluorescence in fluorescence microscopy. J Histochem Cytochem 2002;50:437-9.
38. Saper CB. A guide to the perplexed on the specificity of antibodies. J Histochem Cytochem 2009;57:1-5.
39. Chattopadhyay PK, Roederer M. Cytometry: today's technology and tomorrow's horizons. Methods 2012;57:251-8.
40. Roederer M, Brenchley JM, Betts MR, De Rosa SC. Flow cytometric analysis of vaccine responses: how many colors are enough? Clin Immunol 2004;110:199-205.
41. O'Brien MC, Bolton WE. Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry 1995;19:243-55.
42. Perfetto SP, Chattopadhyay PK, Roederer MZR. Seventeen-colour flow cytometry: unravelling the immune system. Nat Rev Immunol 2004;4:648-55.
43. Perfetto SP, Ambrozak D, Nguyen R, Chattopadhyay P, Roederer M. Quality assurance for polychromatic flow cytometry. Nat Protoc 2006;1:1522-30.
44. Keeney M, Gratama JW, Chin-Yee IH, Sutherland DR. Isotype controls in the analysis of lymphocytes and CD34+ stem and progenitor cells by flow cytometry-time to let go!. Cytometry 1998;34:280-3.
45. Stewart CC, Stewart SJ. Cell preparation for the identification of leukocytes. Methods Cell Biol 1994;41:39-60.
46. O'Gorman MR, Thomas J. Isotype controls-time to let go? Cytometry 1999;38:78-80.
47. Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother 1996;5:213-26.
48. De Rosa SC, Herzenberg LA, Roederer M. 11-color, 13-parameter flow cytometry: identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat Med 2001;7:245-8.
49. Schultz C, Rott C, Temming P, von Puttkammer J, Bucsky P. Influence of specimen age and use of different negative controls in determination of intracytoplasmic levels of cytokines after whole-blood culture assay. Clin Diagn Lab Immunol 2002;9:295-8.
50. Gratama JW, Sutherland DR, Keeney M, Papa S. Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells. J Biol Regul Homeost Agents 2001;15:14-22.
51. McLaughlin BE, Baumgarth N, Bigos M et al. Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: panel design by an empiric approach. Cytometry A 2008;73:400-10.
52. Lanza F, Healy L, Sutherland DR. Structural and functional features of the CD34 antigen: an update. J Biol Regul Homeost Agents 2001;15:1-13.
53. Le Roy C, Varin-Blank N, Ajchenbaum-Cymbalista F, Letestu R. Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism. Cytometry A 2009;75:882-90.
54. Nomura L, Maino VC, Maecker HT. Standardization and optimization of multiparameter intracellular cytokine staining. Cytometry A 2008;73:984-91.
55. Krishan A, Ganapathi R. Laser flow cytometric studies on the intracellular fluorescence of anthracyclines. Cancer Res 1980;40:3895-900.
56. Krishan A, Sridhar KS, Davila E, Vogel C, Sternheim W. Patterns of anthracycline retention modulation in human tumor cells. Cytometry 1987;8:306-14.
57. Aghaeepour N, Finak G, Hoos H et al. Critical assessment of automated flow cytometry data analysis techniques. Nat Methods 2013;10:228-38.
58. Lo K, Brinkman RR, Gottardo R. Automated gating of flow cytometry data via robust model-based clustering. Cytometry A 2008;73:321-32.
59. Zare H, Shooshtari P, Gupta A, Brinkman RR. Data reduction for spectral clustering to analyze high throughput flow cytometry data. BMC Bioinformatics 2010;11:403.
60. Roederer M, Nozzi JL, Nason MC. SPICE: exploration and analysis of post-cytometric complex multivariate datasets. Cytometry A 2011;79:167-74.
61. Aghaeepour N, Chattopadhyay PK, Ganesan A et al. Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays. Bioinformatics 2012;28:1009-16.
62. Aghaeepour N, Nikolic R, Hoos HH, Brinkman RR. Rapid cell population identification in flow cytometry data. Cytometry A 2011;79:6-13.
63. Bashashati A, Brinkman RR. A survey of flow cytometry data analysis methods. Adv Bioinformatics 2009;2009:584603.
64. Sugar IP, Sealfon SC. Misty Mountain clustering: application to fast unsupervised flow cytometry gating. BMC Bioinformatics 2010;11:502.