Improved compensation in flow cytometry by multivariable optimization

Cytometry Part A

Volumn 79 A, Issue 5, 2011, Pages 356-360

  Sugár, István P ^a   González Lergier, Joanna ^a   Sealfon, Stuart C ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Compensation in flow cytometry; Dendritic cells; Optimization; Spillover coefficients

Indexed keywords

ALGORITHM; ARTICLE; CYTOLOGY; DENDRITIC CELL; FLOW CYTOMETRY; METHODOLOGY; MULTIVARIATE ANALYSIS; STAINING;

ALGORITHMS; DENDRITIC CELLS; FLOW CYTOMETRY; MULTIVARIATE ANALYSIS; STAINING AND LABELING;

EID: 79955140386     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.21062     Document Type: Article

References (5)

1. Roederer M. Compensation in flow cytometry. Curr Protocol Cytometry 2002: 1.14.1-1.14.20.
2. Roederer M, Murphy RF. Cell-by-cell autofluorescence correction for low signal-to-noise systems: Application to epidermal growth factor endocytosis by 3T3 fibroblasts. Cytometry 1986; 7: 558-565.
3. Sugar IP, Sealfon SC. Misty Mountain clustering: application to fast unsupervised flow cytometry gating: BMC Bioinformatics 2010; 11: 502.
4. Roederer M. Spectral compensation for flow cytometry: Visualization artifacts, limitations, and caveats. Cytometry 2001; 45: 194-205.
5. Press WH, Flannery BP, Teukolsky SA, Vetterling WT. Numerical Recipes. Cambridge: Cambridge University Press; 1989.