Selecting fluorochrome conjugates for maximum sensitivity

Cytometry Part A

Volumn 62, Issue 2, 2004, Pages 169-173

  Maecker, Holden T ^a   Frey, Tom ^a   Nomura, Laurel E ^a   Trotter, Joe ^a  

^a BD BIOSCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLOPHYCOCYANIN; CD8 ANTIGEN; FLUOROCHROME; GAMMA INTERFERON;

BRIGHTNESS; DATA ANALYSIS; DIGITAL CYTOMETRY; DRUG CONJUGATION; FLOW CYTOMETRY; LUMINESCENCE; POPULATION DIFFERENTIATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVIEW; SENSITIVITY AND SPECIFICITY; STAINING; STATISTICAL ANALYSIS; VARIANCE;

FALSE POSITIVE REACTIONS; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; FLUOROMETRY; SENSITIVITY AND SPECIFICITY; SPECTRUM ANALYSIS;

EID: 9644276717     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.20092     Document Type: Review

References (8)

1. De Rosa SC, Brenchley JM, Roederer M. Beyond six colors: a new era in flow cytometry. Nat Med 2003;9:112-117.
2. Shapiro HM. Practical flow cytometry. 4th ed. New York: Wiley-Liss; 2003.
3. Baumgarth N, Roederer M. A practical approach to multicolor flow cytometry for immunophenotyping. J Immunol Methods 2000;243:77-97.
4. Bigos M, Baumgarth N, Jager GC, Herman OC, Nozaki T, Stovel RT, Parks DR, Herzenberg LA. Nine color eleven parameter immunophenotyping using three laser flow cytometry. Cytometry 1999;36:36-45.
5. Roederer M. Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry 2001;45:194-205.
6. Roederer M, De Rosa S, Gerstein R, Anderson M, Bigos M, Stovel R, Nozaki T, Parks D, Herzenberg L. 8 Color, 10-parameter flow cytometry to elucidate complex leukocyte heterogeneity. Cytometry 1997;29:328-339.
7. Frey T. How the FACSCalibur and FACSVantage work and why it matters. In: Diamond RA, DeMaggio S, editors. In living color. Berlin: Springer; 2000. p 15-38.
8. Bigos M, Stovel R, Parks D. Evaluating multi-color fluorescence data quality among different instruments and different laser powers-methods and results (abstract). Cytometry 2004;59A:42.