Introduction to cell-hydrogel mechanosensing

Interface Focus

Volumn 4, Issue 2, 2014, Pages

  Ahearne, Mark ^a  

^a TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

Adhesion; Bioreactor; Contraction; Hydrogel; Mechanobiology; Tissue engineering

Indexed keywords

EID: 84894357175     PISSN: 20428898     EISSN: 20428901     Source Type: Journal    
DOI: 10.1098/rsfs.2013.0038     Document Type: Review

References (196)

1. Lutz W, Sanderson W, Scherbov S. 2008 The coming acceleration of global population ageing. Nature 451, 716-719. (doi:10.1038/nature06516)
2. Langer R, Vacanti JP. 1993 Tissue engineering. Science 260, 920-926. (doi:10.1126/science. 8493529)
3. Xu Y, Shi Y, Ding S. 2008 A chemical approach to stem-cell biology and regenerative medicine. Nature 453, 338-344. (doi:10.1038/nature07042)
4. Mano JF et al. 2007 Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends. J. R. Soc. Interface 4, 999-1030. (doi:10.1098/rsif. 2007.0220)
5. Lynch AP, Ahearne M. 2013 Strategies for developing decellularized corneal scaffolds. Exp. Eye Res. 108, 42-47. (doi:10.1016/j.exer.2012.12.012)
6. Sukmana I. 2012 Bioactive polymer scaffold for fabrication of vascularized engineering tissue. J. Artif. Organs 15, 215-224. (doi:10.1007/s10047-012-0644-6)
7. Hutmacher DW. 2000 Scaffolds in tissue engineering bone and cartilage. Biomaterials 21, 2529-2543. (doi:10.1016/S0142-9612(00)00121-6)
8. Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK. 2002 Electrospun nanofibrous structure: a novel scaffold for tissue engineering. J. Biomed. Mater. Res. 60, 613-621. (doi:10.1002/jbm.10167)
9. Nicodemus GD, Bryant SJ. 2008 Cell encapsulation in biodegradable hydrogels for tissue engineering applications. Tissue Eng. B 14, 149-165. (doi:10. 1089/ten.teb.2007.0332)
10. Jhon MS, Andrade JD. 1973 Water and hydrogels. J. Biomed. Mater. Res. 7, 509-522. (doi:10.1002/jbm.820070604)
11. Molinaro G, Leroux JC, Damas J, Adam A. 2002 Biocompatibility of thermosensitive chitosan-based hydrogels: an in vivo experimental approach to injectable biomaterials. Biomaterials 23, 2717-2722. (doi:10.1016/S0142-9612(02)00004-2)
12. Noguchi T, Yamamuro T, Oka M, Kumar P, Kotoura Y, Hyon S, Ikadat Y. 1991 Poly(vinyl alcohol) hydrogel as an artificial articular cartilage: evaluation of biocompatibility. J. Appl. Biomater. 2, 101-107. (doi:10.1002/jab.770020205)
13. Jen AC, Wake MC, Mikos AG. 1996 Review: hydrogels for cell immobilization. Biotechnol. Bioeng. 50, 357-364. (doi:10.1002/(SICI)1097-0290(19960520)50:4<357::AID-BIT2>3.3.CO;2-F)
14. Lee KY, Mooney DJ. 2001 Hydrogels for tissue engineering. Chem. Rev. 101, 1869-1879. (doi:10.1021/cr000108x)
15. Ahearne M, Wilson SL, Liu KK, Rauz S, El Haj AJ, Yang Y. 2010 Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model. Exp. Eye Res. 91, 584-591. (doi:10.1016/j. exer.2010.07.013)
16. Bott K, Upton Z, Schrobback K, Ehrbar M, Hubbell JA, Lutolf MP, Rizzi SC. 2010 The effect of matrix characteristics on fibroblast proliferation in 3D gels. Biomaterials 31, 8454-8464. (doi:10.1016/j. biomaterials.2010.07.046)
17. Noth U et al. 2005 Anterior cruciate ligament constructs fabricated from human mesenchymal stem cells in a collagen type I hydrogel. Cytotherapy 7, 447-455. (doi:10.1080/14653240500319093)
18. Ahearne M, Yang Y, El Haj AJ, Then KY, Liu KK. 2005 Characterizing the viscoelastic properties of thin hydrogel-based constructs for tissue engineering applications. J. R. Soc. Interface 2, 455-463. (doi:10.1098/rsif.2005.0065)
19. Bader RA. 2008 Synthesis and viscoelastic characterization of novel hydrogels generated via photopolymerization of 1,2-epoxy-5-hexene modified poly(vinyl alcohol) for use in tissue replacement. Acta Biomater. 4, 967-975. (doi:10. 1016/j.actbio.2008.02.015)
20. Suh JK, Matthew HW. 2000 Application of chitosanbased polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials 21, 2589-2598. (doi:10.1016/S0142-9612(00)00126-5)
21. Bahney CS, Hsu CW, Yoo JU, West JL, Johnstone B. 2011 A bioresponsive hydrogel tuned to chondrogenesis of human mesenchymal stem cells. FASEB J. 25, 1486-1496. (doi:10.1096/fj.10-165514)
22. Oliveira JT, Santos TC, Martins L, Silva MA, Marques AP, Castro AG, Neves NM, Reis RL. 2009 Performance of new gellan gum hydrogels combined with human articular chondrocytes for cartilage regeneration when subcutaneously implanted in nude mice. J. Tissue Eng. Regen. Med. 3, 493-500. (doi:10.1002/term.184)
23. Hu K, Shi H, Zhu J, Deng D, Zhou G, Zhang W, Cao Y, Liu W. 2010 Compressed collagen gel as the scaffold for skin engineering. Biomed. Microdevices 12, 627-635. (doi:10.1007/s10544-010-9415-4)
24. Hartmann-Fritsch F et al. In press. Collagen hydrogels strengthened by biodegradable meshes are a basis for dermo-epidermal skin grafts intended to reconstitute human skin in a one-step surgical intervention. J. Tissue Eng. Regen. Med. (doi:10.1002/term.1665)
25. Sun G et al. 2011 Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing. Proc. Natl Acad. Sci. USA 108, 20 976-20 981. (doi:10.1073/pnas.1115973108)
26. Reno F, Rizzi M, Cannas M. 2012 Effect of a gelatin hydrogel incorporating epiregulin on human keratinocyte growth. J. Biomater. Sci. Polym. Ed. 23, 2025-2038. (doi:10.1163/092050611X603872)
27. Hunt NC, Shelton RM, Grover L. 2009 An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co-culture. Biotechnol. J. 4, 730-737. (doi:10.1002/biot.200800292)
28. Li FF et al. 2003 Cellular and nerve regeneration within a biosynthetic extracellular matrix for corneal transplantation. Proc. Natl Acad. Sci. USA 100, 15 346-15 351. (doi:10.1073/pnas.0136820100)
29. Alaminos M, Del Carmen Sanchez-Quevedo M, Munoz-Avila JI, Serrano D, Medialdea S, Carreras I, Campos A. 2006 Construction of a complete rabbit cornea substitute using a fibrin-agarose scaffold. Invest. Ophthalmol. Vis. Sci. 47, 3311-3317. (doi:10.1167/iovs.05-1647)
30. Liu Y, Chan-Park MB. 2009 Hydrogel based on interpenetrating polymer networks of dextran and gelatin for vascular tissue engineering. Biomaterials 30, 196-207. (doi:10.1016/j.biomaterials.2008.09.041)
31. Ferreira LS, Gerecht S, Fuller J, Shieh HF, Vunjak-Novakovic G, Langer R. 2007 Bioactive hydrogel scaffolds for controllable vascular differentiation of human embryonic stem cells. Biomaterials 28, 2706-2717. (doi:10.1016/j.biomaterials.2007. 01.021)
32. Deng C, Zhang P, Vulesevic B, Kuraitis D, Li F, Yang AF, Griffith M, Ruel M, Suuronen EJ. 2010 A collagen-chitosan hydrogel for endothelial differentiation and angiogenesis. Tissue Eng. A 16, 3099-3109. (doi:10.1089/ten.tea.2009.0504)
33. Dahlmann J et al. 2013 Fully defined in situ crosslinkable alginate and hyaluronic acid hydrogels for myocardial tissue engineering. Biomaterials 34, 940-951. (doi:10.1016/j.biomaterials.2012.10.008)
34. Haque T, Chen H, Ouyang W, Martoni C, Lawuyi B, Urbanska AM, Prakash S. 2005 In vitro study of alginate-chitosan microcapsules: an alternative to liver cell transplants for the treatment of liver failure. Biotechnol. Lett. 27, 317-322. (doi:10. 1007/s10529-005-0687-3)
35. Bhattacharya M et al. 2012 Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture. J. Control. Release 164, 291-298. (doi:10. 1016/j.jconrel.2012.06.039)
36. Kim M, Lee JY, Jones CN, Revzin A, Tae G. 2010 Heparin-based hydrogel as a matrix for encapsulation and cultivation of primary hepatocytes. Biomaterials 31, 3596-3603. (doi:10. 1016/j.biomaterials.2010.01.068)
37. Xu W et al. 2013 Human iPSC-derived neural crest stem cells promote tendon repair in a rat patellar tendon window defect model. Tissue Eng. A 19, 2439-2451. (doi:10.1089/ten.tea.2012.0453)
38. Brown RA, Wiseman M, Chuo CB, Cheema U, Nazhat SN. 2005 Ultrarapid engineering of biomimetic materials and tissues: fabrication of nano-and microstructures by plastic compression. Adv. Funct. Mater. 15, 1762-1770. (doi:10.1002/adfm. 200500042)
39. Lee JH, Yu HS, Lee GS, Ji A, Hyun JK, Kim HW. 2011 Collagen gel three-dimensional matrices combined with adhesive proteins stimulate neuronal differentiation of mesenchymal stem cells. J. R. Soc. Interface 8, 998-1010. (doi:10.1098/rsif.2010.0613)
40. Gillette BM, Jensen JA, Tang B, Yang GJ, Bazargan-Lari A, Zhong M, Sia SK. 2008 In situ collagen assembly for integrating microfabricated threedimensional cell-seeded matrices. Nat. Mater. 7, 636-640. (doi:10.1038/nmat2203)
41. Janmey PA, Winer JP, Weisel JW. 2009 Fibrin gels and their clinical and bioengineering applications. J. R. Soc. Interface 6, 1-10. (doi:10.1098/rsif. 2008.0327)
42. Ahearne M, Buckley CT, Kelly DJ. 2011 A growth factor delivery system for chondrogenic induction of infrapatellar fat pad-derived stem cells in fibrin hydrogels. Biotechnol. Appl. Biochem. 58, 345-352. (doi:10.1002/bab.45)
43. Ahmed TA, Dare EV, Hincke M. 2008 Fibrin: a versatile scaffold for tissue engineering applications. Tissue Eng. B 14, 199-215. (doi:10.1089/ten.teb. 2007.0435)
44. Hanjaya-Putra D, Shen YI, Wilson A, Fox-Talbot K, Khetan S, Burdick JA, Steenbergen C, Gerecht S. 2013 Integration and regression of implanted engineered human vascular networks during deep wound healing. Stem Cells Transl. Med. 2, 297-306. (doi:10.5966/sctm.2012-0111)
45. Khetan S, Guvendiren M, Legant WR, Cohen DM, Chen CS, Burdick JA. 2013 Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels. Nat. Mater. 12, 458-465. (doi:10.1038/nmat3586)
46. Baier Leach J, Bivens KA, Patrick Jr CW, Schmidt CE. 2003 Photocrosslinked hyaluronic acid hydrogels: natural, biodegradable tissue engineering scaffolds. Biotechnol. Bioeng. 82, 578-589. (doi:10.1002/bit.10605)
47. Kuo CK, Ma PX. 2001 Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: part 1. Structure, gelation rate and mechanical properties. Biomaterials 22, 511-521. (doi:10. 1016/S0142-9612(00)00201-5)
48. Alsberg E, Anderson KW, Albeiruti A, Franceschi RT, Mooney DJ. 2001 Cell-interactive alginate hydrogels for bone tissue engineering. J. Dent. Res. 80, 2025-2029. (doi:10.1177/00220345010800111501)
49. Liu Z et al. 2012 The influence of chitosan hydrogel on stem cell engraftment, survival and homing in the ischemic myocardial microenvironment. Biomaterials 33, 3093-3106. (doi:10.1016/j. biomaterials.2011.12.044)
50. Hong Y, Song H, Gong Y, Mao Z, Gao C, Shen J. 2007 Covalently crosslinked chitosan hydrogel: properties of in vitro degradation and chondrocyte encapsulation. Acta Biomater. 3, 23-31. (doi:10. 1016/j.actbio.2006.06.007)
51. Ahearne M, Kelly DJ. 2013 A comparison of fibrin, agarose and gellan gum hydrogels as carriers of stem cells and growth factor delivery microspheres for cartilage regeneration. Biomed. Mater. 8, 035004. (doi:10.1088/1748-6041/8/3/035004)
52. Luo Y, Shoichet MS. 2004 A photolabile hydrogel for guided three-dimensional cell growth and migration. Nat. Mater. 3, 249-253. (doi:10. 1038/nmat1092)
53. Buckley CT, Vinardell T, Thorpe SD, Haugh MG, Jones E, McGonagle D, Kelly DJ. 2010 Functional properties of cartilaginous tissues engineered from infrapatellar fat pad-derived mesenchymal stem cells. J. Biomech. 43, 920-926. (doi:10.1016/j. jbiomech.2009.11.005)
54. Orgel JP, Miller A, Irving TC, Fischetti RF, Hammersley AP, Wess TJ. 2001 The in situ supermolecular structure of type I collagen. Structure 9, 1061-1069. (doi:10.1016/S0969-2126(01)00669-4)
55. Bella J, Eaton M, Brodsky B, Berman HM. 1994 Crystal and molecular structure of a collagen-like peptide at 1.9 A resolution. Science 266, 75-81. (doi:10.1126/science.7695699)
56. Buehler MJ. 2006 Nature designs tough collagen: explaining the nanostructure of collagen fibrils. Proc. Natl Acad. Sci. USA 103, 12 285-12 290. (doi:10.1073/pnas.0603216103)
57. Buehler MJ. 2008 Nanomechanics of collagen fibrils under varying cross-link densities: atomistic and continuum studies. J. Mech. Behav. Biomed. Mater. 1, 59-67. (doi:10.1016/j.jmbbm.2007.04.001)
58. Rowley JA, Madlambayan G, Mooney DJ. 1999 Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 20, 45-53. (doi:10.1016/S0142-9612(98)00107-0)
59. Wang L, Shelton RM, Cooper PR, Lawson M, Triffitt JT, Barralet JE. 2003 Evaluation of sodium alginate for bone marrow cell tissue engineering. Biomaterials 24, 3475-3481. (doi:10.1016/S0142-9612(03)00167-4)
60. Temenoff JS, Athanasiou KA, LeBaron RG, Mikos AG. 2002 Effect of poly(ethylene glycol) molecular weight on tensile and swelling properties of oligo(poly(ethylene glycol) fumarate) hydrogels for cartilage tissue engineering. J. Biomed. Mater. Res. 59, 429-437. (doi:10.1002/jbm.1259)
61. Burdick JA, Anseth KS. 2002 Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering. Biomaterials 23, 4315-4323. (doi:10.1016/S0142-9612(02) 00176-X)
62. Zhu J. 2010 Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering. Biomaterials 31, 4639-4656. (doi:10.1016/j. biomaterials.2010.02.044)
63. Chiu YC, Kocagoz S, Larson JC, Brey EM. 2013 Evaluation of physical and mechanical properties of porous poly (ethylene glycol)-co-(L-lactic acid) hydrogels during degradation. PLoS ONE 8, e60728. (doi:10.1371/journal.pone.0060728)
64. Kisiday J, Jin M, Kurz B, Hung H, Semino C, Zhang S, Grodzinsky AJ. 2002 Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: implications for cartilage tissue repair. Proc. Natl Acad. Sci. USA 99, 9996-10 001. (doi:10.1073/pnas.142309999)
65. Holmes TC, de Lacalle S, Su X, Liu G, Rich A, Zhang S. 2000 Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds. Proc. Natl Acad. Sci. USA 97, 6728-6733. (doi:10.1073/pnas.97.12.6728)
66. Zhang S, Holmes T, Lockshin C, Rich A. 1993 Spontaneous assembly of a self-complementary oligopeptide to form a stable macroscopic membrane. Proc. Natl Acad. Sci. USA 90, 3334-3338. (doi:10.1073/pnas.90.8.3334)
67. Zhang S, Holmes TC, DiPersio CM, Hynes RO, Su X, Rich A. 1995 Self-complementary oligopeptide matrices support mammalian cell attachment. Biomaterials 16, 1385-1393. (doi:10.1016/0142-9612(95)96874-Y)
68. Dasgupta A, Mondal JH, Das D. 2013 Peptide hydrogels. RSC Adv. 3, 9117-9149. (doi:10.1039/c3ra40234g)
69. Hynes RO. 1992 Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69, 11-25. (doi:10.1016/0092-8674(92)90115-S)
70. Hynes RO. 1987 Integrins: a family of cell surface receptors. Cell 48, 549-554. (doi:10.1016/0092-8674(87)90233-9)
71. Ruoslahti E, Pierschbacher MD. 1987 New perspectives in cell adhesion: RGD and integrins. Science 238, 491-497. (doi:10.1126/science. 2821619)
72. Cukierman E, Pankov R, Stevens DR, Yamada KM. 2001 Taking cell-matrix adhesions to the third dimension. Science 294, 1708-1712. (doi:10.1126/science.1064829)
73. Hynes RO. 2002 Integrins: bidirectional, allosteric signaling machines. Cell 110, 673-687. (doi:10. 1016/S0092-8674(02)00971-6)
74. Morgan MR, Humphries MJ, Bass MD. 2007 Synergistic control of cell adhesion by integrins and syndecans. Nat. Rev. Mol. Cell Biol. 8, 957-969. (doi:10.1038/nrm2289)
75. Gates J, Peifer M. 2005 Can 1000 reviews be wrong? Actin, alpha-catenin, and adherens junctions. Cell 123, 769-772. (doi:10.1016/j.cell.2005.11.009)
76. Chen S, Lewallen M, Xie T. 2013 Adhesion in the stem cell niche: biological roles and regulation. Development 140, 255-265. (doi:10.1242/dev. 083139)
77. Yang F, Williams CG, Wang DA, Lee H, Manson PN, Elisseeff J. 2005 The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells. Biomaterials 26, 5991-5998. (doi:10.1016/j. biomaterials.2005.03.018)
78. Zustiak SP, Durbal R, Leach JB. 2010 Influence of cell-adhesive peptide ligands on poly(ethylene glycol) hydrogel physical, mechanical and transport properties. Acta Biomater. 6, 3404-3414. (doi:10. 1016/j.actbio.2010.03.040)
79. Steward AJ, Wagner DR, Kelly DJ. 2013 The pericellular environment regulates cytoskeletal development and the differentiation of mesenchymal stem cells and determines their response to hydrostatic pressure. Eur. Cell Mater. 25, 167-178.
80. Steward AJ, Thorpe SD, Vinardell T, Buckley CT, Wagner DR, Kelly DJ. 2012 Cell-matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure. Acta Biomater. 8, 2153-2159. (doi:10.1016/j.actbio.2012.03.016)
81. Cavalcanti-Adam EA, Volberg T, Micoulet A, Kessler H, Geiger B, Spatz JP. 2007 Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands. Biophys. J. 92, 2964-2974. (doi:10.1529/biophysj.106.089730)
82. Tzeranis DS, Roy A, So PT, Yannas IV. 2010 An optical method to quantify the density of ligands for cell adhesion receptors in three-dimensional matrices. J. R. Soc. Interface 7(Suppl. 5), S649-S661. (doi:10.1098/rsif.2010.0321.focus)
83. Gobin AS, West JL. 2002 Cell migration through defined, synthetic ECM analogs. FASEB J. 16, 751-753. (doi:10.1096/fj.01-0759fje)
84. Zaman MH, Trapani LM, Sieminski AL, Mackellar D, Gong H, Kamm RD, Wells A, Lauffenburger DA, Matsudaira P. 2006 Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis. Proc. Natl Acad. Sci. USA 103, 10 889-10 894. (doi:10.1073/pnas.0604460103)
85. Burgess BT, Myles JL, Dickinson RB. 2000 Quantitative analysis of adhesion-mediated cell migration in three-dimensional gels of RGD-grafted collagen. Ann. Biomed. Eng. 28, 110-118. (doi:10. 1114/1.259)
86. Mattila PK, Lappalainen P. 2008 Filopodia: molecular architecture and cellular functions. Nat. Rev. Mol. Cell Biol. 9, 446-454. (doi:10.1038/nrm2406)
87. Wehrle-Haller B, Imhof BA. 2003 Actin, microtubules and focal adhesion dynamics during cell migration. Int. J. Biochem. Cell Biol. 35, 39-50. (doi:10.1016/S1357-2725(02)00071-7)
88. Pantaloni D, Le Clainche C, Carlier MF. 2001 Mechanism of actin-based motility. Science 292, 1502-1506. (doi:10.1126/science.1059975)
89. Cheng SY, Heilman S, Wasserman M, Archer S, Shuler ML, Wu M. 2007 A hydrogel-based microfluidic device for the studies of directed cell migration. Lab Chip 7, 763-769. (doi:10.1039/b618463d)
90. Choi YS, Vincent LG, Lee AR, Kretchmer KC, Chirasatitsin S, Dobke MK, Engler AJ. 2012 The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices. Biomaterials 33, 6943-6951. (doi:10.1016/j. biomaterials.2012.06.057)
91. Vincent LG, Choi YS, Alonso-Latorre B, del Alamo JC, Engler AJ. 2013 Mesenchymal stem cell durotaxis depends on substrate stiffness gradient strength. Biotechnol. J. 8, 472-484. (doi:10.1002/biot. 201200205)
92. Hadjipanayi E, Mudera V, Brown RA. 2009 Guiding cell migration in 3D: a collagen matrix with graded directional stiffness. Cell Motil. Cytoskeleton 66, 121-128. (doi:10.1002/cm.20331)
93. Miller JS, Shen CJ, Legant WR, Baranski JD, Blakely BL, Chen CS. 2010 Bioactive hydrogels made from step-growth derived PEG-peptide macromers. Biomaterials 31, 3736-3743. (doi:10.1016/j. biomaterials.2010.01.058)
94. Meshel AS, Wei Q, Adelstein RS, Sheetz MP. 2005 Basic mechanism of three-dimensional collagen fibre transport by fibroblasts. Nat. Cell Biol. 7, 157-164. (doi:10.1038/ncb1216)
95. Wakatsuki T, Elson EL. 2003 Reciprocal interactions between cells and extracellular matrix during remodeling of tissue constructs. Biophys. Chem. 100, 593-605. (doi:10.1016/S0301-4622(02) 00308-3)
96. Bell E, Ivarsson B, Merrill C. 1979 Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc. Natl Acad. Sci. USA 76, 1274-1278. (doi:10.1073/pnas.76.3.1274)
97. Ahearne M, Liu KK, El Haj AJ, Then KY, Rauz S, Yang Y. 2010 Online monitoring of the mechanical behavior of collagen hydrogels: influence of corneal fibroblasts on elastic modulus. Tissue Eng. C 16, 319-327. (doi:10.1089/ten.tec. 2008.0650)
98. Chung C, Anderson E, Pera RR, Pruitt BL, Heilshorn SC. 2012 Hydrogel crosslinking density regulates temporal contractility of human embryonic stem cell-derived cardiomyocytes in 3D cultures. Soft Matter 8, 10 141-10 148. (doi:10.1039/c2sm26082d)
99. Ahearne M, Bagnaninchi PO, Yang Y, El Haj AJ. 2008 Online monitoring of collagen fibre alignment in tissue-engineered tendon by PSOCT. J. Tissue Eng. Regen. Med. 2, 521-524. (doi:10.1002/term.124)
100. East E, de Oliveira DB, Golding JP, Phillips JB. 2010 Alignment of astrocytes increases neuronal growth in three-dimensional collagen gels and is maintained following plastic compression to form a spinal cord repair conduit. Tissue Eng. A 16, 3173-3184. (doi:10.1089/ten.tea.2010.0017)
101. Rhee S, Grinnell F. 2007 Fibroblast mechanics in 3D collagen matrices. Adv. Drug Deliv. Rev. 59, 1299-1305. (doi:10.1016/j.addr.2007.08.006)
102. Brown RA, Prajapati R, McGrouther DA, Yannas IV, Eastwood M. 1998 Tensional homeostasis in dermal fibroblasts: mechanical responses to mechanical loading in three-dimensional substrates. J. Cell. Physiol. 175, 323-332. (doi:10.1002/(SICI)1097-4652(199806)175:3<323::AID-JCP10>3.0.CO;2-6)
103. Kharkar PM, Kiick KL, Kloxin AM. 2013 Designing degradable hydrogels for orthogonal control of cell microenvironments. Chem. Soc. Rev. 42, 7335-7372. (doi:10.1039/c3cs60040h)
104. Ragoowansi R, Khan U, Brown RA, McGrouther DA. 2003 Differences in morphology, cytoskeletal architecture and protease production between zone II tendon and synovial fibroblasts in vitro. J. Hand Surg. Br. 28, 465-470. (doi:10.1016/S0266-7681 (03)00140-2)
105. Han S et al. 2010 Molecular mechanism of type I collagen homotrimer resistance to mammalian collagenases. J. Biol. Chem. 285, 22 276-22 281. (doi:10.1074/jbc.M110.102079)
106. Mauch C, Adelmann-Grill B, Hatamochi A, Krieg T. 1989 Collagenase gene expression in fibroblasts is regulated by a three-dimensional contact with collagen. FEBS Lett. 250, 301-305. (doi:10.1016/0014-5793(89)80743-4)
107. Aimes RT, Quigley JP. 1995 Matrix metalloproteinase-2 is an interstitial collagenase. Inhibitor-free enzyme catalyzes the cleavage of collagen fibrils and soluble native type I collagen generating the specific 3/4-and 1/4-length fragments. J. Biol. Chem. 270, 5872-5876. (doi:10.1074/jbc.270.11.5872)
108. Lutolf MP, Lauer-Fields JL, Schmoekel HG, Metters AT, Weber FE, Fields GB, Hubbell JA. 2003 Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: engineering cellinvasion characteristics. Proc. Natl Acad. Sci. USA 100, 5413-5418. (doi:10.1073/pnas.0737381100)
109. Patterson J, Hubbell JA. 2010 Enhanced proteolytic degradation of molecularly engineered PEG hydrogels in response to MMP-1 and MMP-2. Biomaterials 31, 7836-7845. (doi:10.1016/j. biomaterials.2010.06.061)
110. Bian L, Zhai DY, Tous E, Rai R, Mauck RL, Burdick JA. 2011 Enhanced MSC chondrogenesis following delivery of TGF-beta3 from alginate microspheres within hyaluronic acid hydrogels in vitro and in vivo. Biomaterials 32, 6425-6434. (doi:10.1016/j. biomaterials.2011.05.033)
111. DeFail AJ, Chu CR, Izzo N, Marra KG. 2006 Controlled release of bioactive TGF-beta 1 from microspheres embedded within biodegradable hydrogels. Biomaterials 27, 1579-1585. (doi:10.1016/j. biomaterials.2005.08.013)
112. Adhikari AS, Chai J, Dunn AR. 2011 Mechanical load induces a 100-fold increase in the rate of collagen proteolysis by MMP-1. J. Am. Chem. Soc. 133, 1686-1689. (doi:10.1021/ja109972p)
113. Ellsmere JC, Khanna RA, Lee JM. 1999 Mechanical loading of bovine pericardium accelerates enzymatic degradation. Biomaterials 20, 1143-1150. (doi:10. 1016/S0142-9612(99)00013-7)
114. Chang SW, Flynn BP, Ruberti JW, Buehler MJ. 2012 Molecular mechanism of force induced stabilization of collagen against enzymatic breakdown. Biomaterials 33, 3852-3859. (doi:10.1016/j. biomaterials.2012.02.001)
115. Hou X, Han QH, Hu D, Tian L, Guo CM, Du HJ, Zhang P, Wang Y-S, Hui Y-N. 2009 Mechanical force enhances MMP-2 activation via p38 signaling pathway in human retinal pigment epithelial cells. Graefes Arch. Clin. Exp. Ophthalmol. 247, 1477-1486. (doi:10.1007/s00417-009-1135-1)
116. Zhang L, Li X, Bi LJ. 2011 Alterations of collagen-I, MMP-1 and TIMP-1 in the periodontal ligament of diabetic rats under mechanical stress. J. Periodontal Res. 46, 448-455. (doi:10.1111/j.1600-0765.2011.01359.x)
117. Cummins PM, von Offenberg Sweeney N, Killeen MT, Birney YA, Redmond EM, Cahill PA. 2007 Cyclic strain-mediated matrix metalloproteinase regulation within the vascular endothelium: a force to be reckoned with. Am. J. Physiol. Heart Circ. Physiol. 292, H28-H42. (doi:10.1152/ajpheart.00304.2006)
118. Nauman EA, Ebenstein DM, Hughes KF, Pruitt L, Halloran BP, Bikle DD, Keaveny TM. 2002 Mechanical and chemical characteristics of mineral produced by basic fibroblast growth factor-treated bone marrow stromal cells in vitro. Tissue Eng. 8, 931-939. (doi:10.1089/107632702320934038)
119. Kazakia GJ, Nauman EA, Ebenstein DM, Halloran BP, Keaveny TM. 2006 Effects of in vitro bone formation on the mechanical properties of a trabeculated hydroxyapatite bone substitute. J. Biomed. Mater. Res. A 77, 688-699. (doi:10.1002/jbm.a.30644)
120. Gkioni K, Leeuwenburgh SC, Douglas TE, Mikos AG, Jansen JA. 2010 Mineralization of hydrogels for bone regeneration. Tissue Eng. B 16, 577-585. (doi:10.1089/ten.teb.2010.0462)
121. Zhong C, Chu CC. 2012 Biomimetic mineralization of acid polysaccharide-based hydrogels: towards porous 3-dimensional bone-like biocomposites. J. Mater. Chem. 22, 6080-6087. (doi:10.1039/c2jm15610e)
122. Du C, Cui FZ, Zhang W, Feng QL, Zhu XD, de Groot K. 2000 Formation of calcium phosphate/collagen composites through mineralization of collagen matrix. J. Biomed. Mater. Res. 50, 518-527. (doi:10.1002/(SICI)1097-4636(20000615)50:4<518::AID-JBM7> 3.0.CO;2-W)
123. Douglas TE et al. 2012 Enzymatic mineralization of hydrogels for bone tissue engineering by incorporation of alkaline phosphatase. Macromol. Biosci. 12, 1077-1089. (doi:10.1002/mabi. 201100501)
124. Burdick JA, Mason MN, Hinman AD, Thorne K, Anseth KS. 2002 Delivery of osteoinductive growth factors from degradable PEG hydrogels influences osteoblast differentiation and mineralization. J. Control. Release 83, 53-63. (doi:10.1016/S0168-3659(02)00181-5)
125. Hu JC, Athanasiou KA. 2005 Low-density cultures of bovine chondrocytes: effects of scaffold material and culture system. Biomaterials 26, 2001-2012. (doi:10.1016/j.biomaterials.2004.06.038)
126. Williams GM, Klein TJ, Sah RL. 2005 Cell density alters matrix accumulation in two distinct fractions and the mechanical integrity of alginatechondrocyte constructs. Acta Biomater. 1, 625-633. (doi:10.1016/j.actbio.2005.07.009)
127. Wan LQ, Jiang J, Miller DE, Guo XE, Mow VC, Lu HH. 2011 Matrix deposition modulates the viscoelastic shear properties of hydrogel-based cartilage grafts. Tissue Eng. A 17, 1111-1122. (doi:10.1089/ten.tea. 2010.0379)
128. Bissell MJ, Aggeler J. 1987 Dynamic reciprocity: how do extracellular matrix and hormones direct gene expression? Prog. Clin. Biol. Res. 249, 251-262.
129. Roskelley CD, Bissell MJ. 1995 Dynamic reciprocity revisited: a continuous, bidirectional flow of information between cells and the extracellular matrix regulates mammary epithelial cell function. Biochem. Cell Biol. 73, 391-397. (doi:10.1139/o95-046)
130. Discher DE, Janmey P, Wang YL. 2005 Tissue cells feel and respond to the stiffness of their substrate. Science 310, 1139-1143. (doi:10.1126/science. 1116995)
131. Engler AJ, Sen S, Sweeney HL, Discher DE. 2006 Matrix elasticity directs stem cell lineage specification. Cell 126, 677-689. (doi:10.1016/j.cell. 2006.06.044)
132. Peyton SR, Putnam AJ. 2005 Extracellular matrix rigidity governs smooth muscle cell motility in a biphasic fashion. J. Cell. Physiol. 204, 198-209. (doi:10.1002/jcp.20274)
133. Lo CM, Wang HB, Dembo M, Wang YL. 2000 Cell movement is guided by the rigidity of the substrate. Biophys. J. 79, 144-152. (doi:10.1016/S0006-3495(00)76279-5)
134. Even-Ram S, Artym V, Yamada KM. 2006 Matrix control of stem cell fate. Cell 126, 645-647. (doi:10.1016/j.cell.2006.08.008)
135. Saha K, Keung AJ, Irwin EF, Li Y, Little L, Schaffer DV, Healy KE. 2008 Substrate modulus directs neural stem cell behavior. Biophys. J. 95, 4426-4438. (doi:10.1529/biophysj.108.132217)
136. Sanz-Ramos P, Mora G, Vicente-Pascual M, Ochoa I, Alcaine C, Moreno R, Doblaré M, Izal-Azcárate I. 2013 Response of sheep chondrocytes to changes in substrate stiffness from 2 to 20 Pa: effect of cell passaging. Connect. Tissue Res. 54, 159-166. (doi:10.3109/03008207.2012.762360)
137. Forte G et al. 2012 Substrate stiffness modulates gene expression and phenotype in neonatal cardiomyocytes in vitro. Tissue Eng. A 18, 1837-1848. (doi:10.1089/ten.tea.2011.0707)
138. Bhana B, Iyer RK, Chen WL, Zhao R, Sider KL, Likhitpanichkul M, Simmons CA, Radisic M. 2010 Influence of substrate stiffness on the phenotype of heart cells. Biotechnol. Bioeng. 105, 1148-1160. (doi:10.1002/bit.22647)
139. Hopp I, Michelmore A, Smith LE, Robinson DE, Bachhuka A, Mierczynska A, Vasilev K. 2013 The influence of substrate stiffness gradients on primary human dermal fibroblasts. Biomaterials 34, 5070-5077. (doi:10.1016/j.biomaterials.2013.03.075)
140. Jones RR, Hamley IW, Connon CJ. 2012 Ex vivo expansion of limbal stem cells is affected by substrate properties. Stem Cell Res. 8, 403-409. (doi:10.1016/j.scr.2012.01.001)
141. Trappmann B et al. 2012 Extracellular-matrix tethering regulates stem-cell fate. Nat. Mater. 11, 642-649. (doi:10.1038/nmat3339)
142. Marklein RA, Burdick JA. 2010 Spatially controlled hydrogel mechanics to modulate stem cell interactions. Soft Matter 6, 136-143. (doi:10.1039/b916933d)
143. Banerjee A, Arha M, Choudhary S, Ashton RS, Bhatia SR, Schaffer DV, Kane RS. 2009 The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells. Biomaterials 30, 4695-4699. (doi:10.1016/j. biomaterials.2009.05.050)
144. Cha C, Jeong JH, Shim J, Kong H. 2011 Tuning the dependency between stiffness and permeability of a cell encapsulating hydrogel with hydrophilic pendant chains. Acta Biomater. 7, 3719-3728. (doi:10.1016/j.actbio.2011.06.017)
145. Hadjipanayi E, Mudera V, Brown RA. 2009 Close dependence of fibroblast proliferation on collagen scaffold matrix stiffness. J. Tissue Eng. Regen. Med. 3, 77-84. (doi:10.1002/term.136)
146. Levis HJ, Brown RA, Daniels JT. 2010 Plastic compressed collagen as a biomimetic substrate for human limbal epithelial cell culture. Biomaterials 31, 7726-7737. (doi:10.1016/j.biomaterials.2010.07.012)
147. Alekseeva T, Hadjipanayi E, Abou Neel EA, Brown RA. 2012 Engineering stable topography in dense biomimetic 3D collagen scaffolds. Eur. Cells Mater. 23, 28-40.
148. Kureshi A, Cheema U, Alekseeva T, Cambrey A, Brown R. 2010 Alignment hierarchies: engineering architecture from the nanometre to the micrometre scale. J. R. Soc. Interface 7(Suppl. 6), S707-S716. (doi:10.1098/rsif.2010.0346.focus)
149. Mi S, Chen B, Wright B, Connon CJ. 2010 Ex vivo construction of an artificial ocular surface by combination of corneal limbal epithelial cells and a compressed collagen scaffold containing keratocytes. Tissue Eng. A 16, 2091-2100. (doi:10.1089/ten.tea. 2009.0748)
150. Ahearne M, Yang Y, Then KY, Liu KK. 2008 Nondestructive mechanical characterisation of UVA/riboflavin crosslinked collagen hydrogels. Br. J. Ophthalmol. 92, 268-271. (doi:10.1136/bjo.2007.130104)
151. Moffat KL, Marra KG. 2004 Biodegradable poly(ethylene glycol) hydrogels crosslinked with genipin for tissue engineering applications. J. Biomed. Mater. Res. B 71, 181-187. (doi:10. 1002/jbm.b.30070)
152. Wylie RG, Shoichet MS. 2011 Three-dimensional spatial patterning of proteins in hydrogels. Biomacromolecules 12, 3789-3796. (doi:10.1021/bm201037j)
153. Girton TS, Dubey N, Tranquillo RT. 1999 Magneticinduced alignment of collagen fibrils in tissue equivalents. Methods Mol. Med. 18, 67-73. (doi:10.1385/0-89603-516-6:67)
154. Torbet J, Ronziere MC. 1984 Magnetic alignment of collagen during self-assembly. Biochem. J. 219, 1057-1059.
155. Ahearne M, Siamantouras E, Yang Y, Liu KK. 2009 Mechanical characterization of biomimetic membranes by micro-shaft poking. J. R. Soc. Interface 6, 471-478. (doi:10.1098/rsif.2008.0317)
156. Sarvestani AS, He X, Jabbari E. 2007 Viscoelastic characterization and modeling of gelation kinetics of injectable in situ cross-linkable poly(lactide-co-ethylene oxide-co-fumarate) hydrogels. Biomacromolecules 8, 406-415. (doi:10.1021/bm060648p)
157. Hyland LL, Taraban MB, Feng Y, Hammouda B, Yu YB. 2012 Viscoelastic properties and nanoscale structures of composite oligopeptide-polysaccharide hydrogels. Biopolymers 97, 177-188. (doi:10.1002/bip.21722)
158. Yang Y, Bagnaninchi PO, Ahearne M, Wang RK, Liu KK. 2007 A novel optical coherence tomographybased micro-indentation technique for mechanical characterization of hydrogels. J. R. Soc. Interface 4, 1169-1173. (doi:10.1098/rsif.2007.1044)
159. Demol J, Lambrechts D, Geris L, Schrooten J, Van Oosterwyck H. 2011 Towards a quantitative understanding of oxygen tension and cell density evolution in fibrin hydrogels. Biomaterials 32, 107-118. (doi:10.1016/j.biomaterials.2010.08.093)
160. Wilson SL, Wimpenny I, Ahearne M, Ruaz S, El Haj AJ, Yang Y. 2012 Chemical and topographical effects on cell differentiation and matrix elasticity in a corneal stromal layer model. Adv. Funct. Mater. 22, 3641-3649. (doi:10.1002/adfm. 201200655)
161. Kai D, Prabhakaran MP, Stahl B, Eblenkamp M, Wintermantel E, Ramakrishna S. 2012 Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications. Nanotechnology 23, 095705. (doi:10. 1088/0957-4484/23/9/095705)
162. Yang Y, Wimpenny I, Ahearne M. 2011 Portable nanofiber meshes dictate cell orientation throughout three-dimensional hydrogels. Nanomedicine 7, 131-136. (doi:10.1016/j.nano. 2010.12.011)
163. Tonsomboon K, Oyen ML. 2013 Composite electrospun gelatin fiber-alginate gel scaffolds for mechanically robust tissue engineered cornea. J. Mech. Behav. Biomed. Mater. 21, 185-194. (doi:10.1016/j.jmbbm.2013.03.001)
164. Geng H, Song H, Qi J, Cui D. 2011 Sustained release of VEGF from PLGA nanoparticles embedded thermo-sensitive hydrogel in full-thickness porcine bladder acellular matrix. Nanoscale Res. Lett. 6, 312. (doi:10.1186/1556-276X-6-312)
165. Lim SM, Oh SH, Lee HH, Yuk SH, Im GI, Lee JH. 2010 Dual growth factor-releasing nanoparticle/hydrogel system for cartilage tissue engineering. J. Mater. Sci. Mater. Med. 21, 2593-2600. (doi:10. 1007/s10856-010-4118-1)
166. Gaharwar AK, Dammu SA, Canter JM, Wu CJ, Schmidt G. 2011 Highly extensible, tough, and elastomeric nanocomposite hydrogels from poly(ethylene glycol) and hydroxyapatite nanoparticles. Biomacromolecules 12, 1641-1650. (doi:10.1021/bm200027z)
167. Tran PA, Zhang L, Webster TJ. 2009 Carbon nanofibers and carbon nanotubes in regenerative medicine. Adv. Drug Deliv. Rev. 61, 1097-1114. (doi:10.1016/j.addr.2009.07.010)
168. Zhao B, Hu H, Mandal SK, Haddon RC. 2005 A bone mimic based on the self-assembly of hydroxyapatite on chemically functionalized single-walled carbon nanotubes. Chem. Mater. 17, 3235-3241. (doi:10. 1021/cm0500399)
169. Shin SR et al. 2012 Carbon nanotube reinforced hybrid microgels as scaffold materials for cell encapsulation. ACS Nano 6, 362-372. (doi:10. 1021/nn203711s)
170. Xie F, Weiss P, Chauvet O, Le Bideau J, Tassin JF. 2010 Kinetic studies of a composite carbon nanotube-hydrogel for tissue engineering by rheological methods. J. Mater. Sci. Mater. Med. 21, 1163-1168. (doi:10.1007/s10856-009-3984-x)
171. Dong C, Kashon ML, Lowry D, Dordick JS, Reynolds SH, Rojanasakul Y, Sargent LM, Dinu CZ. 2013 Exposure to carbon nanotubes leads to changes in the cellular biomechanics. Adv. Healthcare Mater. 2, 945-951. (doi:10.1002/adhm.201200430)
172. Martin I, Wendt D, Heberer M. 2004 The role of bioreactors in tissue engineering. Trends Biotechnol. 22, 80-86. (doi:10.1016/j.tibtech.2003.12.001)
173. Portner R, Nagel-Heyer S, Goepfert C, Adamietz P, Meenen NM. 2005 Bioreactor design for tissue engineering. J. Biosci. Bioeng. 100, 235-245. (doi:10.1263/jbb.100.235)
174. Chen HC, Hu YC. 2006 Bioreactors for tissue engineering. Biotechnol. Lett. 28, 1415-1423. (doi:10.1007/s10529-006-9111-x)
175. Mauck RL, Soltz MA, Wang CC, Wong DD, Chao PH, Valhmu WB, Hung CT, Ateshian GA. 2000 Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. J. Biomech. Eng. 122, 252-260. (doi:10.1115/1.429656)
176. Mauck RL, Byers BA, Yuan X, Tuan RS. 2007 Regulation of cartilaginous ECM gene transcription by chondrocytes and MSCs in 3D culture in response to dynamic loading. Biomech. Model. Mechanobiol. 6, 113-125. (doi:10.1007/s10237-006-0042-1)
177. Doroski DM, Levenston ME, Temenoff JS. 2010 Cyclic tensile culture promotes fibroblastic differentiation of marrow stromal cells encapsulated in poly(ethylene glycol)-based hydrogels. Tissue Eng. A 16, 3457-3466. (doi:10.1089/ten.tea.2010.0233)
178. Webb K, Hitchcock RW, Smeal RM, Li W, Gray SD, Tresco PA. 2006 Cyclic strain increases fibroblast proliferation, matrix accumulation, and elastic modulus of fibroblast-seeded polyurethane constructs. J. Biomech. 39, 1136-1144. (doi:10. 1016/j.jbiomech.2004.08.026)
179. Puetzer J, Williams J, Gillies A, Bernacki S, Loboa EG. 2013 The effects of cyclic hydrostatic pressure on chondrogenesis and viability of human adipose-and bone marrow-derived mesenchymal stem cells in three-dimensional agarose constructs. Tissue Eng. A 19, 299-306. (doi:10.1089/ten.tea.2012.0015)
180. Meyer EG, Buckley CT, Steward AJ, Kelly DJ. 2011 The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells. J. Mech. Behav. Biomed. Mater. 4, 1257-1265. (doi:10.1016/j.jmbbm.2011. 04.012)
181. Chen T, Buckley M, Cohen I, Bonassar L, Awad HA. 2012 Insights into interstitial flow, shear stress, and mass transport effects on ECM heterogeneity in bioreactor-cultivated engineered cartilage hydrogels. Biomech. Model. Mechanobiol. 11, 689-702. (doi:10.1007/s10237-011-0343-x)
182. Eniwumide JO, Lee DA, Bader DL. 2009 The development of a bioreactor to perfuse radially-confined hydrogel constructs: design and characterization of mass transport properties. Biorheology 46, 417-437. (doi:10. 3233/BIR-2009-0552)
183. Orr AW, Helmke BP, Blackman BR, Schwartz MA. 2006 Mechanisms of mechanotransduction. Dev. Cell 10, 11-20. (doi:10.1016/j.devcel.2005.12.006)
184. Starr DA, Fridolfsson HN. 2010 Interactions between nuclei and the cytoskeleton are mediated by SUNKASH nuclear-envelope bridges. Annu. Rev. Cell Dev. Biol. 26, 421-444. (doi:10.1146/annurev-cellbio-100109-104037)
185. Crisp M, Liu Q, Roux K, Rattner JB, Shanahan C, Burke B, Stahl PD, Hodzic D. 2006 Coupling of the nucleus and cytoplasm: role of the LINC complex. J. Cell Biol. 172, 41-53. (doi:10.1083/jcb. 200509124)
186. Khatau SB et al. 2012 The distinct roles of the nucleus and nucleus-cytoskeleton connections in three-dimensional cell migration. Sci. Rep. 2, 488. (doi:10.1038/srep00488)
187. Sosa BA, Rothballer A, Kutay U, Schwartz TU. 2012 LINC complexes form by binding of three KASH peptides to domain interfaces of trimeric SUN proteins. Cell 149, 1035-1047. (doi:10.1016/j.cell. 2012.03.046)
188. (2+)-conducting ion channels in mechanicallyinduced signal transduction of airway epithelial cells. J. Cell Sci. 107, 3037-3044.
189. Stojkovska J, Bugarski B, Obradovic B. 2010 Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering. J. Mater. Sci. Mater. Med. 21, 2869-2879. (doi:10.1007/s10856-010-4135-0)
190. Gonen-Wadmany M, Gepstein L, Seliktar D. 2004 Controlling the cellular organization of tissueengineered cardiac constructs. Ann. N.Y. Acad. Sci. 1015, 299-311. (doi:10.1196/annals.1302.025)
191. Rathbone SR, Glossop JR, Gough JE, Cartmell SH. 2012 Cyclic tensile strain upon human mesenchymal stem cells in 2D and 3D culture differentially influences CCNL2, WDR61 and BAHCC1 gene expression levels. J. Mech. Behav. Biomed. Mater. 11, 82-91. (doi:10.1016/j.jmbbm.2012.01.019)
192. Hall AC. 1999 Differential effects of hydrostatic pressure on cation transport pathways of isolated articular chondrocytes. J. Cell. Physiol. 178, 197-204. (doi:10.1002/(SICI)1097-4652(199902) 178:2<197::AID-JCP9>3.0.CO;2-3)
193. Elder BD, Athanasiou KA. 2009 Hydrostatic pressure in articular cartilage tissue engineering: from chondrocytes to tissue regeneration. Tissue Eng. B 15, 43-53. (doi:10.1089/ten.teb.2008.0435)
194. Toyoda T, Seedhom BB, Kirkham J, Bonass WA. 2003 Upregulation of aggrecan and type II collagen mRNA expression in bovine chondrocytes by the application of hydrostatic pressure. Biorheology 40, 79-85.
195. Toyoda T, Seedhom BB, Yao JQ, Kirkham J, Brookes S, Bonass WA. 2003 Hydrostatic pressure modulates proteoglycan metabolism in chondrocytes seeded in agarose. Arthritis Rheum 48, 2865-2872. (doi:10. 1002/art.11250)
196. Malone AM, Batra NN, Shivaram G, Kwon RY, You L, Kim CH, Rodriguez J, Jair K, Jacobs CR. 2007 The role of actin cytoskeleton in oscillatory fluid flowinduced signaling in MC3T3-E1 osteoblasts. Am. J. Physiol. Cell Physiol. 292, C1830-C1836. (doi:10.1152/ajpcell.00352.2005)