Multifunctional antitumor molecule 5′-triphosphate siRNA combining glutaminase silencing and RIG-I activation

International Journal of Cancer

Volumn 134, Issue 8, 2014, Pages 1958-1971

  Meng, Gang ^a   Xia, Mao ^a   Xu, Chun ^a   Yuan, Dongmei ^a   Schnurr, Max ^b   Wei, Jiwu ^a,c  

^a MEDICAL SCHOOL OF NANJING UNIVERSITY   (China)

^b LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^c NANJING UNIVERSITY   (China)

Author keywords

apoptosis; cancer cell; glutaminase; RIG I; ROS; siRNA

Indexed keywords

APOPTOSIS; CANCER CELL; GLUTAMINASE; RIG-I; ROS; SIRNA;

APOPTOSIS; CELL LINE, TUMOR; CELL SURVIVAL; DEAD-BOX RNA HELICASES; ENZYME ACTIVATION; FEMALE; GLIOMA; GLUTAMINASE; HELA CELLS; HUMANS; LUNG NEOPLASMS; MITOCHONDRIA; NEOPLASMS; PANCREATIC NEOPLASMS; REACTIVE OXYGEN SPECIES; RNA INTERFERENCE; RNA, SMALL INTERFERING; UTERINE CERVICAL NEOPLASMS;

EID: 84893772398     PISSN: 00207136     EISSN: 10970215     Source Type: Journal    
DOI: 10.1002/ijc.28416     Document Type: Article

References (42)

1. Hanahan D, Weinberg RA,. Hallmarks of cancer: the next generation. Cell 2011; 144: 646-74.
2. Vander Heiden MG, Cantley LC, Thompson CB,. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science 2009; 324: 1029-33.
3. Tennant DA, Duran RV, Gottlieb E,. Targeting metabolic transformation for cancer therapy. Nat Rev Cancer 2010; 10: 267-77.
4. Erickson JW, Cerione RA,. Glutaminase: a hot spot for regulation of cancer cell metabolism? Oncotarget 2010; 1: 734-40.
5. DeBerardinis RJ, Mancuso A, Daikhin E, et al. Beyond aerobic glycolysis: transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis. Proc Natl Acad Sci USA 2007; 104: 19345-50.
6. Shanware NP, Mullen AR, DeBerardinis RJ, et al. Glutamine: pleiotropic roles in tumor growth and stress resistance. J Mol Med (Berl) 2011; 89: 229-36.
7. Márquez J, de la Oliva AR, Matés JM, et al. Glutaminase: a multifaceted protein not only involved in generating glutamate. Neurochem Int 2006; 48: 465-71.
8. Matés J, Segura J, Martín-Rufián M, et al. Glutaminase isoenzymes as key regulators in metabolic and oxidative stress against cancer. Curr Mol Med 2013; 13: 514-34.
9. Suzuki S, Tanaka T, Poyurovsky MV, et al. Phosphate-activated glutaminase (GLS2), a p53-inducible regulator of glutamine metabolism and reactive oxygen species. Proc Natl Acad Sci USA 2010; 107: 7461-6.
10. Wang JB, Erickson JW, Fuji R, et al. Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Cancer Cell 2010; 18: 207-19.
11. Gao P, Tchernyshyov I, Chang TC, et al. c-Myc suppression of miR-23a/b enhances mitochondrial glutaminase expression and glutamine metabolism. Nature 2009; 458: 762-5.
12. Szeliga M, Obara-Michlewska M,. Glutamine in neoplastic cells: focus on the expression and roles of glutaminases. Neurochem Int 2009; 55: 71-5.
13. Aledo JC, Gómez-Fabre PM, Olalla L, et al. Identification of two human glutaminase loci and tissue-specific expression of the two related genes. Mamm Genome 2000; 11: 1107-10.
14. Elgadi KM, Meguid RA, Qian M, et al. Cloning and analysis of unique human glutaminase isoforms generated by tissue-specific alternative splicing. Physiol Genom 1999; 1: 51-62. (Pubitemid 129622451)
15. Son J, Lyssiotis CA, Ying H, et al. Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway. Nature 2013; 496: 101-5.
16. Szatrowski TP, Nathan CF,. Production of large amounts of hydrogen peroxide by human tumor cells. Cancer Res 1991; 51: 794-8.
17. Trachootham D, Alexandre J, Huang P,. Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat Rev Drug Discov 2009; 8: 579-91.
18. Schumacker PT,. Reactive oxygen species in cancer cells: live by the sword, die by the sword. Cancer Cell 2006; 10: 175-6. (Pubitemid 44311072)
19. Matés JM, Segura JA, Alonso FJ, et al. Oxidative stress in apoptosis and cancer: an update. Arch Toxicol 2012; 86: 1649-65.
20. Le A, Lane, AN, Hamaker, M, et al. Glucose-independent glutamine metabolism via TCA cycling for proliferation and survival in B cells. Cell Metab 2012; 15: 110-21.
21. Lora J, Alonso FJ, Segura JA, et al. Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells. Eur J Biochem 2004; 271: 4298-306. (Pubitemid 39506759)
22. Robinson MM, McBryant SJ, Tsukamoto T, et al. Novel mechanism of inhibition of rat kidney-type glutaminase by bis-2-(5-phenylacetamido-1,2,4- thiadiazol-2-yl)ethyl sulfide (BPTES). Biochem J 2007; 406: 407-14. (Pubitemid 47425447)
23. Lu W, Pelicano H, Huang P,. Cancer metabolism: is glutamine sweeter than glucose? Cancer Cell 2010; 18: 199-200.
24. Poeck H, Besch R, Maihoefer C, et al. 5′-Triphosphate-siRNA: turning gene silencing and Rig-I activation against melanoma. Nat Med 2008; 14: 1256-63.
25. Hornung V, Ellegast J, Kim S, et al. 5′-Triphosphate RNA is the ligand for RIG-I. Science 2006; 314: 994-7.
26. Pichlmair A, Schulz O, Tan CP, et al. RIG-I-mediated antiviral responses to single-stranded RNA bearing 5'-phosphates. Science 2006; 314: 997-1001. (Pubitemid 44749946)
27. Dunn GP, Koebel CM, Schreiber RD,. Interferons, immunity and cancer immunoediting. Nat Rev Immunol 2006; 6: 836-48. (Pubitemid 44631652)
28. Besch R, Poeck H, Hohenauer T, et al. Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon-independent apoptosis in human melanoma cells. J Clin Invest 2009; 119: 2399-411.
29. Ellermeier J, Wei J, Duewell P, et al. Therapeutic efficacy of bifunctional siRNA combining TGF-β1 silencing with RIG-I activation in pancreatic cancer. Cancer Res 2013; 73: 1709-20.
30. Vander Heiden MG, Lunt SY, Dayton TL, et al. Metabolic pathway alterations that support cell proliferation. Cold Spring Harb Symp Quant Biol 2011; 76: 325-34.
31. Twig G, Elorza A, Molina AJ, et al. Fission and selective fusion govern mitochondrial segregation and elimination by autophagy. EMBO J 2008; 27: 433-46. (Pubitemid 351161653)
32. Donadio AC, Lobo C, Tosina M, et al. Antisense glutaminase inhibition modifies the O-GlcNAc pattern and flux through the hexosamine pathway in breast cancer cells. J Cell Biochem 2008; 103: 800-11. (Pubitemid 351240616)
33. Matés JM, Perez-Gomez C, Nunez de Castro I, et al. Glutamine and its relationship with intracellular redox status, oxidative stress and cell proliferation/death. Int J Biochem Cell Biol 2002; 34: 439-58. (Pubitemid 34242758)
34. Yudkoff M, Pleasure D, Cregar L, et al. Glutathione turnover in cultured astrocytes: studies with [15N]glutamate. J Neurochem 1990; 55: 137-45.
35. Nathan C, Ding A,. SnapShot: reactive oxygen intermediates (ROI). Cell 2010; 140: 951-e2.
36. Cairns RA, Harris IS, Mak TW,. Regulation of cancer cell metabolism. Nat Rev Cancer 2011; 11: 85-95.
37. Gonzalez P, Mader I, Tchoghandjian A, et al. Impairment of lysosomal integrity by B10, a glycosylated derivative of betulinic acid, leads to lysosomal cell death and converts autophagy into a detrimental process. Cell Death Differ 2012; 19: 1337-46.
38. Dang CV, Le A, Gao, P,. MYC-induced cancer cell energy metabolism and therapeutic opportunities. Clin Cancer Res 2009; 15: 6479-83.
39. Hardie DG, Ross FA, Hawley SA,. AMPK: a nutrient and energy sensor that maintains energy homeostasis. Nat Rev Mol Cell Biol 2012; 13: 251-62.
40. Zmijewski JW, Banerjee S, Bae H, et al. Exposure to hydrogen peroxide induces oxidation and activation of AMP-activated protein kinase. J Biol Chem 2010; 285: 33154-64.
41. Hawley SA, Ross FA, Chevtzoff C, et al. Use of cells expressing gamma subunit variants to identify diverse mechanisms of AMPK activation. Cell Metab 2010; 11: 554-65.
42. Sanli T, Rashid A, Liu C, et al. Ionizing radiation activates AMP-activated kinase (AMPK): a target for radiosensitization of human cancer cells. Int J Radiat Oncol Biol Phys 2010; 78: 221-9.