Improvement of NADPH bioavailability in Escherichia coli by replacing NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase GapA with NADP+-dependent GapB from Bacillus subtilis and addition of NAD kinase

Journal of Industrial Microbiology and Biotechnology

Volumn 40, Issue 12, 2013, Pages 1449-1460

  Wang, Yipeng ^a   San, Ka Yiu ^b   Bennett, George N ^a  

^a RICE UNIVERSITY   (United States)

^b Rice University   (United States)

Author keywords

Cofactor engineering; E. coli; NAD kinase; NADPdependent GAPDH; NADPH bioavailability; Reducing equivalent

Indexed keywords

2 HALOACRYLATE REDUCTASE; CYCLOHEXANONE; CYCLOHEXANONE MONOOXYGENASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; LYCOPENE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE KINASE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL STRAIN; BIOAVAILABILITY; BIOSYNTHESIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GAPA GENE; GAPB GENE; GENE OVEREXPRESSION; NONHUMAN; OXIDATION; REACTION ANALYSIS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

BACILLUS SUBTILIS; BIOLOGICAL AVAILABILITY; CAROTENOIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); GLYCOLYSIS; METABOLOME; MUTATION; NAD; NADP; OXIDATION-REDUCTION; OXYGENASES; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN SUBUNITS;

EID: 84892831820     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-013-1335-x     Document Type: Article

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