Improved polyhydroxybutyrate production by Saccharomyces cerevisiae through the use of the phosphoketolase pathway

Biotechnology and Bioengineering

Volumn 110, Issue 8, 2013, Pages 2216-2224

  Kocharin, Kanokarn ^a   Siewers, Verena ^a   Nielsen, Jens ^a  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

Author keywords

Acetyl CoA; NADPH; Phosphoketolase pathway; Polyhydroxybutyrate

Indexed keywords

ACETYL-COA; BACTERIAL BIOPOLYMERS; CENTRAL CARBON METABOLISMS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; HETEROLOGOUS EXPRESSION; NADPH; PHOSPHOKETOLASE; POLYHYDROXYBUTYRATE;

BIOCHEMISTRY; BIOLOGICAL MATERIALS; BIOSYNTHESIS; ETHANOL; GENE EXPRESSION; METABOLISM;

YEAST;

ACETYL COENZYME A; ALCOHOL; BIOPOLYMER; GLYCEROL; PHOSPHOKETOLASE; POLYHYDROXYBUTYRATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; ASPERGILLUS NIDULANS; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOSYNTHESIS; CARBON METABOLISM; DEGRADATION; HETEROLOGOUS EXPRESSION; METABOLIC ENGINEERING; NONHUMAN; PLASMID; SACCHAROMYCES CEREVISIAE; STREPTOCOCCUS MUTANS;

ACETYL COENZYME A; ALDEHYDE-LYASES; ASPERGILLUS NIDULANS; BIOTECHNOLOGY; GLYCEROL; HYDROXYBUTYRATES; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; NADP; POLYESTERS; SACCHAROMYCES CEREVISIAE; STREPTOCOCCUS MUTANS;

BACTERIA (MICROORGANISMS); EMERICELLA NIDULANS; SACCHAROMYCES CEREVISIAE; STREPTOCOCCUS MUTANS;

EID: 84879603106     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24888     Document Type: Article

References (27)

1. Blank LM, Sauer U. 2004. TCA cycle activity in Saccharomyces cerevisiae is a function of the environmentally determined specific growth and glucose uptake rates. Microbiology 150(Pt 4):1085-1093.
2. Boeke J, Xu H, Fink G. 1988. A general method for the chromosomal amplification of genes in yeast. Science 239(4837):280-282.
3. Bro C, Regenberg B, Forster J, Nielsen J. 2006. In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production. Metab Eng 8(2):102-111.
4. Bruinenberg PM. 1986. The NADP(H) redox couple in yeast metabolism. Antonie Van Leeuwenhoek 52(5):411-429.
5. Bruinenberg PM, Vandijken JP, Scheffers WA. 1983. A theoretical-analysis of NADPH production and consumption in yeasts. J Gen Microbiol 129:953-964.
6. Carlson R, Srienc F. 2006. Effects of recombinant precursor pathway variations on poly[(R)-3-hydroxybutyrate] synthesis in Saccharomyces cerevisiae. J Biotechnol 124(3):561-573.
7. Carlson R, Fell D, Srienc F. 2002. Metabolic pathway analysis of a recombinant yeast for rational strain development. Biotechnol Bioeng 79(2):121-134.
8. Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J. 2013. Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Met Eng 15(0):48-54.
9. Dawes EA, Senior PJ. 1973. The role and regulation of energy reserve polymers in micro-organisms. In: AH, Rose DW, Tempest editors. Advances in microbial physiology. Vol. 10. London and New York: Academic Press. p 135-266.
10. Frick O, Wittmann C. 2005. Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis. Microb Cell Fact 4(1):30.
11. Gietz RD, Woods RA. 2002. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol 350:87-96.
12. Gombert AK, Moreira dos Santos M, Christensen B, Nielsen J. 2001. Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under Different conditions of glucose repression. J Bacteriol 183(4):1441-1451.
13. Grabowska D, Chelstowska A. 2003. The ALD6 gene product is indispensable for providing NADPH in yeast cells lacking glucose-6-phosphate dehydrogenase activity. J Biol Chem 278(16):13984-13988.
14. Karr DB, Waters JK, Emerich DW. 1983. Analysis of poly-beta-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Appl Environ Microbiol 46(6):1339-1344.
15. Kocharin K, Chen Y, Siewers V, Nielsen J. 2012. Engineering of acetyl-CoA metabolism for the improved production of polyhydroxybutyrate in Saccharomyces cerevisiae. AMB Express 2(1):52.
16. Leaf TA, Srienc F. 1998. Metabolic modeling of polyhydroxybutyrate biosynthesis. Biotechnol Bioeng 57(5):557-570.
17. Minard KI, McAlister-Henn L. 2005. Sources of NADPH in yeast vary with carbon source. J Biol Chem 280(48):39890-39896.
18. 13C-based metabolic flux analysis. Appl Microbiol Biotechnol 95(4):1001-1010.
19. Partow S, Siewers V, Bjorn S, Nielsen J, Maury J. 2010. Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast 27(11):955-964.
20. Sambrook J, Russell DW. 2006. The Inoue method for preparation and transformation of competent E. Coli: "Ultra-Competent" cells. Cold Spring Harb Protoc 2006(2):3944.
21. Scalcinati G, Partow S, Siewers V, Schalk M, Daviet L, Nielsen J. 2012. Combined metabolic engineering of precursor and co-factor supply to increase alpha-santalene production by Saccharomyces cerevisiae. Microb Cell Fact 11(1):117.
22. Siso MIG, Freire Picos MA, Cerdan ME. 1996. Reoxidation of the NADPH produced by the pentose phosphate pathway is necessary for the utilization of glucose by Kluyveromyces lactisrag2 mutants. FEBS Lett 387(1):7-10.
23. Sonderegger M, Schumperli M, Sauer U. 2004. Metabolic engineering of a phosphoketolase pathway for pentose catabolism in Saccharomyces cerevisiae. Appl Environ Microbiol 70(5):2892-2897.
24. Tyo KE, Zhou H, Stephanopoulos GN. 2006. High-throughput screen for poly-3-hydroxybutyrate in Escherichia coli and Synechocystis sp. strain PCC6803. Appl. Environ. Microbiol 72(5):3412-3417.
25. 13C-labeled primary metabolites. FEMS Yeast Res 5(6-7):559-568.
26. Vaseghi S, Baumeister A, Rizzi M, Reuss M. 1999. In vivo dynamics of the pentose phosphate pathway in Saccharomyces cerevisiae. Metab Eng 1(2):128-140.
27. Verduyn C, Postma E, Scheffers WA, van Dijken JP. 1990. Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. J Gen Microbiol 136(3):395-403.