Improved NADPH supply for xylitol production by engineered Escherichia coli with glycolytic mutations

Biotechnology Progress

Volumn 27, Issue 2, 2011, Pages 333-341

  Chin, Jonathan W ^a   Cirino, Patrick C ^a  

^a The Pennsylvania State University   (United States)

Author keywords

Cofactor regeneration; Escherichia coli; Metabolic engineering; Resting cells; Xylitol

Indexed keywords

AEROBIC GROWTH; CANDIDA BOIDINII; COFACTOR REGENERATION; E. COLI; METABOLIC ENGINEERING; METABOLIC PATHWAYS; NADH DEHYDROGENASE; NICOTINAMIDE COFACTOR; PENTOSE PHOSPHATE PATHWAY; PHOSPHOFRUCTOKINASE; PHOSPHOGLUCOSE ISOMERASE; RESTING CELLS; SOLUBLE TRANSHYDROGENASE; STRONG CORRELATION; TRANSHYDROGENASES; XYLITOL; XYLOSE REDUCTASE;

AMIDES; ENCODING (SYMBOLS); ENZYME ACTIVITY; ESCHERICHIA COLI; GENE ENCODING; GLUCOSE; METABOLISM; PHYSIOLOGY; STRAIN;

SUGAR SUBSTITUTES;

ALDEHYDE REDUCTASE; GLUCOSE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; XYLITOL; XYLOSE;

ARTICLE; BIOSYNTHESIS; CANDIDA; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; GLYCOLYSIS; METABOLISM; MUTATION;

ALDEHYDE REDUCTASE; CANDIDA; ESCHERICHIA COLI; GLUCOSE; GLYCOLYSIS; MUTATION; NADP; XYLITOL; XYLOSE;

CANDIDA BOIDINII; ESCHERICHIA COLI; INSECTIVORA;

EID: 79954423939     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.559     Document Type: Article

References (54)

1. Cirino PC, Chin JW, Ingram LO. Engineering Escherichia coli for xylitol production from glucose-xylose mixtures. Biotechnol Bioeng. 2006; 95: 1167-1176.
2. Chin JW, Khankal R, Monroe CA, Maranas CD, Cirino PC. Analysis of NADPH supply during xylitol production by engineered Escherichia coli. Biotechnol Bioeng. 2009; 102: 209-220.
3. Duetz WA, van Beilen JB, Witholt B. Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis. Curr Opin Biotechnol. 2001; 12: 419-425.
4. Koeller KM, Wong CH. Enzymes for chemical synthesis. Nature. 2001; 409: 232-240.
5. Schmid A, Dordick JS, Hauer B, Kiener A, Wubbolts M, Witholt B. Industrial biocatalysis today and tomorrow. Nature. 2001; 409: 258-268.
6. Akinterinwa O, Khankal R, Cirino PC. Metabolic engineering for bioproduction of sugar alcohols. Curr Opin Biotechnol. 2008; 19: 461-467.
7. Boghigian BA, Pfeifer BA. Current status, strategies, and potential for the metabolic engineering of heterologous polyketides in Escherichia coli. Biotechnol Lett. 2008; 30: 1323-1330.
8. Das A, Yoon SH, Lee SH, Kim JY, Oh DK, Kim SW. An update on microbial carotenoid production: application of recent metabolic engineering tools. Appl Microbiol Biotechnol. 2007; 77: 505-512.
9. Jarboe LR, Grabar TB, Yomano LP, Shanmugan KT, Ingram LO. Development of ethanologenic bacteria. Adv Biochem Eng Biotechnol. 2007; 108: 237-261.
10. Lee SY, Kim HU, Park JH, Park JM, Kim TY. Metabolic engineering of microorganisms: general strategies and drug production. Drug Discov Today. 2009; 14: 78-88.
11. Neidhardt FC, Curtiss R. Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd ed. Washington, DC: ASM Press; 1996:2 v (xx, 2822, lxxvi).
12. Yan Y, Liao JC. Engineering metabolic systems for production of advanced fuels. J Ind Microbiol Biotechnol. 2009; 36: 471-479.
13. Khankal R, Chin JW, Cirino PC. Role of xylose transporters in xylitol production from engineered Escherichia coli. J Biotechnol. 2008; 134: 246-252.
14. Sauer U, Canonaco F, Heri S, Perrenoud A, Fischer E. The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. J Biol Chem. 2004; 279: 6613-6619.
15. Causey TB, Zhou S, Shanmugam KT, Ingram LO. Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: homoacetate production. Proc Natl Acad Sci USA. 2003; 100: 825-832.
16. Miller JH. A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor: Cold Spring Harbor Press; 1992.
17. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor: Cold Spring Harbor Press; 2001.
18. Haldimann A, Wanner BL. Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J Bacteriol. 2001; 183: 6384-6393.
19. Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. 2006; 2: 2006.0008.
20. Datsenko KA, Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA. 2000; 97: 6640-6645.
21. Walton AZ, Stewart JD. Understanding and improving NADPH-dependent reactions by nongrowing Escherichia coli cells. Biotechnol Prog. 2004; 20: 403-411.
22. Khankal R, Chin JW, Ghosh D, Cirino PC. Transcriptional effects of CRP* expression in Escherichia coli. J Biol Eng. 2009; 3: 13.
23. Bernofsky C, Swan M. An improved cycling assay for nicotinamide adenine dinucleotide. Anal Biochem. 1973; 53: 452-458.
24. Gibon Y, Larher F. Cycling assay for nicotinamide adenine dinucleotides: NaCl precipitation and ethanol solubilization of the reduced tetrazolium. Anal Biochem. 1997; 251: 153-157.
25. Avigad G. Stimulation of yeast phosphofructokinase activity by fructose 2, 6-bisphosphate. Biochem Biophys Res Commun. 1981; 102: 985-991.
26. Reed JL, Vo TD, Schilling CH, Palsson BO. An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR). Genome Biol. 2003; 4: R54.
27. Covert MW, Palsson BO. Transcriptional regulation in constraints-based metabolic models of Escherichia coli. J Biol Chem. 2002; 277: 28058-28064.
28. Canonaco F, Hess TA, Heri S, Wang T, Szyperski T, Sauer U. Metabolic flux response to phosphoglucose isomerase knock-out in Escherichia coli and impact of overexpression of the soluble transhydrogenase UdhA. FEMS Microbiol Lett. 2001; 204: 247-252.
29. Varma A, Palsson BO. Stoichiometric flux balance models quantitatively predict growth and metabolic by-product secretion in wild-type Escherichia coli W3110. Appl Environ Microbiol. 1994; 60: 3724-3731.
30. Babul J. Phosphofructokinases from Escherichia coli. Purification and characterization of the nonallosteric isozyme. J Biol Chem. 1978; 253: 4350-4355.
31. Blangy D, Buc H, Monod J. Kinetics of the allosteric interactions of phosphofructokinase from Escherichia coli. J Mol Biol. 1968; 31: 13-35.
32. Daldal F. Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme. J Mol Biol. 1983; 168: 285-305.
33. Morrissey AT, Fraenkel DG. Selection of fructose 6-phosphate kinase mutants in Escherichia coli. Biochem Biophys Res Commun. 1968; 32: 467-473.
34. Fischer E, Sauer U. Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS. Eur J Biochem. 2003; 270: 880-891.
35. Flores S, de Anda-Herrera R, Gosset G, Bolivar FG. Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway. Biotechnol Bioeng. 2004; 87: 485-494.
36. Kimata K, Tanaka Y, Inada T, Aiba H. Expression of the glucose transporter gene, ptsG, is regulated at the mRNA degradation step in response to glycolytic flux in Escherichia coli. EMBO J. 2001; 20: 3587-3595.
37. Lovingshimer MR, Siegele D, Reinhart GD. Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources. Protein Expr Purif. 2006; 46: 475-482.
38. Morita T, El-Kazzaz W, Tanaka Y, Inada T, Aiba H. Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli. J Biol Chem. 2003; 278: 15608-15614.
39. Lim SJ, Jung YM, Shin HD, Lee YH. Amplification of the NADPH-related genes zwf and gnd for the oddball biosynthesis of PHB in an E. coli transformant harboring a cloned phbCAB operon. J Biosci Bioeng. 2002; 93: 543-549.
40. Lee WH, Park JB, Park K, Kim MD, Seo JH. Enhanced production of epsilon-caprolactone by overexpression of NADPH-regenerating glucose 6-phosphate dehydrogenase in recombinant Escherichia coli harboring cyclohexanone monooxygenase gene. Appl Microbiol Biotechnol. 2007; 76: 329-338.
41. Bestel-Corre G, Boisart C, Chateau M, Gonzalez B, Soucaile P, Figge R, Zink O. Optimised micro-organism strains for NADPH-consuming biosynthetic pathways. US Patent 20070087403, April 19, 2007.
42. Hasona A, Kim Y, Healy FG, Ingram LO, Shanmugam KT. Pyruvate formate lyase and acetate kinase are essential for anaerobic growth of Escherichia coli on xylose. J Bacteriol. 2004; 186: 7593-7600.
43. Jojima T, Omumasaba CA, Inui M, Yukawa H. Sugar transporters in efficient utilization of mixed sugar substrates: current knowledge and outlook. Appl Microbiol Biotechnol. 2010; 85: 471-480.
44. Hibi M, Yukitomo H, Ito M, Mori H. Improvement of NADPH-dependent bioconversion by transcriptome-based molecular breeding. Appl Environ Microbiol. 2007; 73: 7657-7663.
45. Eiteman MA, Altman E. Overcoming acetate in Escherichia coli recombinant protein fermentations. Trends Biotechnol. 2006; 24: 530-536.
46. Gray CT, Wimpenny JW, Mossman MR. Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli. Biochim Biophys Acta. 1966; 117: 33-41.
47. Lara AR, Caspeta L, Gosset G, Bolivar F, Ramirez OT. Utility of an Escherichia coli strain engineered in the substrate uptake system for improved culture performance at high glucose and cell concentrations: an alternative to fed-batch cultures. Biotechnol Bioeng. 2008; 99: 893-901.
48. Wolfe AJ. The acetate switch. Microbiol Mol Biol Rev. 2005; 69: 12-50.
49. Blank LM, Ebert BE, Buhler B, Schmid A. Metabolic capacity estimation of Escherichia coli as a platform for redox biocatalysis: constraint-based modeling and experimental verification. Biotechnol Bioeng. 2008; 100: 1050-1065.
50. + ratio, and the distribution of metabolites in Escherichia coli. Metab Eng. 2002; 4: 238-247.
51. Li ZJ, Cai L, Wu Q, Chen GQ. Overexpression of NAD kinase in recombinant Escherichia coli harboring the phbCAB operon improves poly(3-hydroxybutyrate) production. Appl Microbiol Biotechnol. 2009; 83: 939-947.
52. San KY, Bennett GN, Berrios-Rivera SJ, Vadali RV, Yang YT, Horton E, Rudolph FB, Sariyar B, Blackwood K. Metabolic engineering through cofactor manipulation and its effects on metabolic flux redistribution in Escherichia coli. Metab Eng. 2002; 4: 182-192.
53. Sanchez AM, Andrews J, Hussein I, Bennett GN, San KY. Effect of overexpression of a soluble pyridine nucleotide transhydrogenase (UdhA) on the production of poly(3-hydroxybutyrate) in Escherichia coli. Biotechnol Prog. 2006; 22: 420-425.
54. Shalel-Levanon S, San KY, Bennett GN. Effect of ArcA and FNR on the expression of genes related to the oxygen regulation and glycolysis pathway in Escherichia coli under growth conditions. Biotechnol Bioeng. 2005; 92: 147-159.