Osmotic stress response: Quantification of cell maintenance and metabolic fluxes in a lysine-overproducing strain of Corynebacterium glutamicum

Applied and Environmental Microbiology

Volumn 70, Issue 7, 2004, Pages 4222-4229

  Varela, Cristian A ^a   Baez, Mauricio E ^a   Agosin, Eduardo ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ENVIRONMENTAL IMPACT; FERMENTATION; METABOLISM; OSMOSIS; STRESSES;

BIOPROCESSES; DILUTION RATES; OSMOTIC STRESS RESPONSE;

MICROBIOLOGY;

ADENOSINE TRIPHOSPHATE; LYSINE;

MICROBIOLOGY;

ARTICLE; BACTERIAL OVERGROWTH; BACTERIUM CULTURE; CELL ACTIVATION; CELL GROWTH; CELL METABOLISM; CELL VIABILITY; CORYNEBACTERIUM GLUTAMICUM; DILUTION; ENERGY; FERMENTATION TECHNIQUE; NONHUMAN; OSMOLALITY; OSMOTIC STRESS; PROCESS MONITORING; QUANTITATIVE DIAGNOSIS; STRAIN IDENTIFICATION;

BIOMASS; CORYNEBACTERIUM; ENERGY METABOLISM; LYSINE; OSMOLAR CONCENTRATION; TREHALOSE;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM; UNCULTURED ACTINOMYCETE;

EID: 3242814746     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.7.4222-4229.2004     Document Type: Article

References (35)

1. Bott, M., and A. Niebisch. 2003. The respiratory chain of Corynebacterium glutamicum. J. Biotechnol. 104:129-153.
2. Ciulla, R., and M. Roberts. 1999. Effects of osmotic stress on Methanococcus thermolithotrophicus: 13C-edited 1H-NMR studies of osmolyte turnover. Biochim. Biophys. Acta 1427:193-204.
3. Cuber, R., E. Eleutherio, M. Pereira, and A. Panek. 1997. The role of the trehalose transporter during germination. Biochim. Biophys. Acta 1330:165-171.
4. Dauner, M., T. Storni, and U. Sauer. 2001. Bacillus subtilis metabolism and energetics in carbon-limited and excess-carbon chemostat culture. J. Bacteriol. 183:7308-7317.
5. Eggeling, L., H. Sahm, and A. de Graaf. 1996. Quantifying and directing metabolite flux: application to amino acid overproduction. Adv. Biochem. Eng. Biotechnol. 54:1-30.
6. Farwick, M., R. Siewe, and R. Kramer. 1995. Glycine betaine uptake after hyperosmotic shift in Corynebacterium glutamicum. J. Bacteriol. 177:4690-4695.
7. Frings, E., H. Kunte, and E. Galinski. 1993. Compatible solutes in representatives of the genera Brevibacterium and Corynebacterium: occurrence of tetrahydropyrimidines and glutamamine. FEMS Microbiol. Lett. 109:25-32.
8. González-Sepúlveda, M., and E. Agosin. 2000. Capture of volatile metabolites for tracking the evolution of gibberellic acid in a solid-state culture of Gibberella fujikuroi. Biotechnol. Lett. 22:1849-1854.
9. Guillouet, S., and J. Engasser. 1995. Growth of Corynebacterium glutamicum in glucose-limited continuous cultures under high osmotic pressure. Influence of growth rate on the intracellular accumulation of proline, glutamate and trehalose. Appl. Microbiol. Biotechnol. 44:496-500.
10. Guillouet, S., and J. Engasser. 1995. Sodium and proline accumulation in Corynebacterium glutamicum as a response to an osmotic saline upshock. Appl. Microbiol. Biotechnol. 43:315-320.
11. Kiss, R., and G. Stephanopoulos. 1992. Culture instability of auxotrophic amino-acid producers. Biotechnol. Bioeng. 40:75-85.
12. Kiss, R., and G. Stephanopoulos. 1992. Metabolic characterization of an L-lysine-producing strain by continuous culture. Biotechnol. Bioeng. 39:565-574.
13. Lapujade, P., J. Goergen, and J. Engasser. 1999. Glutamate excretion as a major kinetic bottleneck for the thermally triggered production of glutamic acid by Corynebacterium glutamicum. Metab. Eng. 1:255-261.
14. Martin, D., R. Ciulla, P. Robinson, and M. Roberts. 2000. Switching osmolyte strategies: response of Methanococcus thermalithotrophicus to changes in external NaCl. Biochim. Biophys. Acta 1524:1-10.
15. Ohwada, T., and S. Sagisaka. 1987. An immediate and steep increase in ATP concentration in response to reduced turgor pressure in Escherichia coli B. Arch. Biochem. Biophys. 259:157-163.
16. Ohwada, T., S. Sagisaka, and T. Sato. 1994. An exclusive increase in the concentration of ATP as a result of osmotic stress in Escherichia coli B. Biosci. Biotechnol. Biochem. 58:1512-1513.
17. Oliveira, E., J. Morais, and N. Pereira. 1992. Determination of the energy maintenance coefficient of Zymomonas mobilis. Biotechnol. Lett. 14:1081-1084.
18. Olz, R., K. Larsson, L. Adler, and L. Gustafsson. 1993. Energy flux and osmoregulation of Saccharomyces cerevisiae grown in chemostats under NaCl stress. J. Bacteriol. 175:2205-2213.
19. Oren, A. 1999. Bioenergetic aspects of halophilism. Microbiology and molecular biology reviews. Microbiol. Mol. Biol. Rev. 63:334-348.
20. Petersen, S., A. de Graaf, L. Eggeling, M. Mollney, W. Wiechert, and H. Sahm. 2000. In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum. J. Biol. Chem. 275:35932-35941.
21. Peter-Wendisch, P., V. Wendisch, S. Paul, B. Eikmanns, and H. Sahm. 1997. Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum. Microbiology 143:1095-1103.
22. Pons, A., C. Dussap, C. Péquignot, and J. Gros. 1996. Metabolic flux distribution in Corynebacterium melassecola ATCC 17965 for various carbon sources. Biotechnol. Bioeng. 51:177-189.
23. Regev, R., I. Peri, H. Gilboa, and Y. Avi-Dor. 1990. 13C NMR study of the interrelation between synthesis and uptake of compatible solutes in two moderately halophilic eubacteria, bacterium Bal and Vibrio costicola. Arch. Biochem. Biophys. 278:106-112.
24. Russell, J., and G. Cook. 1995. Energetics of bacterial growth: balance of anabolic and catabolic reactions. Microbiol. Rev. 59:48-62.
25. Shimakata, T., and Y. Minatogawa. 2000. Essential role of trehalose in the synthesis and subsequent metabolism of corynomycolic acid in Corynebacterium matruchotii. Arch. Biochem. Biophys. 380:331-338.
26. Skjerdal, O., H. Sletta, S. Flenstad, K. Josefsen, D. Levine, and T. Ellingsen. 1995. Changes in cell volume, growth and respiration rate in response to hyperosmotic stress of NaCl, sucrose and glutamic acid in Brevibacterium lactofermentum and Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 43:1099-1106.
27. Stephanopoulos, G., A. Aristidou, and J. Nielsen. 1998. Metabolic engineering: principles and methodologies. Academic Press, San Diego, Calif.
28. Stephanopoulos, G., and J. Vallino. 1991. Network rigidity and metabolic engineering in metabolite overproduction. Science 252:1675-1681.
29. Stouthamer, A., and H. van Verseveld. 1987. Microbial energetics should be considered in manipulating metabolism for biotechnological purposes. TIBTECH 5:149-156.
30. Styrvold, O., and A. Strom. 1991. Synthesis, accumulation, and excretion of trehalose in osmotically stressed Escherichia coli K-12 strains: influence of amber suppressors and function of the periplasmic trehalase. J. Bacteriol. 173:1187-1192.
31. Takiguchi, N., H. Shimizu, and S. Shioya. 1997. An online physiological-state recognition system for the lysine fermentation process based on a metabolic reaction model, Biotechnol. Bioeng. 55:170-181.
32. Vallino, J., and G. Stephanopoulos. 1994. Carbon flux distributions at the glucose-6-phosphate branch point in Corynebacterium glutamicum during lysine overproduction, Biotechnol. Prog. 10:327-334.
33. Vallino, J., and G. Stephanopoulos. 1993. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol. Bioeng. 41:633-646.
34. Varela, C., E. Agosin, M. Baez, M. Klapa, and G. Stephanopoulos. 2003. Metabolic flux redistribution in Corynebacterium glutamicum in response to osmotic stress. Appl. Microbiol. Biotechnol. 60:547-555.
35. Ventosa, A., J. Nieto, and A. Oren. 1998. Biology of moderately halophilic aerobic bacteria. Microbiol. Mol. Biol. Rev. 62:504-544.