Inhibition of hepatic fibrosis with artificial microRNA using ultrasound and cationic liposome-bearing microbubbles

Gene Therapy

Volumn 20, Issue 12, 2013, Pages 1140-1148

  Yang, D ^a,b   Gao, Y H ^a   Tan, K B ^a   Zuo, Z X ^a   Yang, W X ^a   Hua, X ^a   Li, P J ^a   Zhang, Y ^a   Wang, G ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b Department of Hepatobiliary Surgery   (China)

Author keywords

artificial microRNA; connective tissue growth factor; gene delivery; hepatic fibrosis; microbubble; ultrasound

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN FIBER; COLLAGEN TYPE 1; CONNECTIVE TISSUE GROWTH FACTOR; LIPOSOME; MICRORNA; TRANSFORMING GROWTH FACTOR BETA1;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; GENE DELIVERY SYSTEM; GENE SILENCING; GENE TARGETING; LIVER FIBROSIS; MICROBUBBLE; NONHUMAN; PARTICLE SIZE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REAL TIME POLYMERASE CHAIN REACTION; ULTRASOUND; WESTERN BLOTTING; ZETA POTENTIAL;

RATTUS;

ACTINS; ANIMALS; CATIONS; CELL LINE; COLLAGEN TYPE I; CONNECTIVE TISSUE GROWTH FACTOR; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; LIPOSOMES; LIVER CIRRHOSIS; MALE; MICROBUBBLES; MICRORNAS; PLASMIDS; RANDOM ALLOCATION; RATS; RATS, SPRAGUE-DAWLEY; RNA INTERFERENCE; TRANSFORMING GROWTH FACTOR BETA1; ULTRASONICS;

EID: 84890123659     PISSN: 09697128     EISSN: 14765462     Source Type: Journal    
DOI: 10.1038/gt.2013.41     Document Type: Article

References (38)

1. Hernandez-Gea V, Friedman SL. Pathogenesis of liver fibrosis. Annu Rev Pathol 2011; 6: 425-456.
2. Lee UE, Friedman SL. Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol 2011; 25: 195-196.
3. Jiao J, Friedman SL, Aloman C. Hepatic fibrosis. Curr Opin Gastroenterol 2009; 25: 223-229.
4. Cheng K, Yang N, Mahato RI. TGF-beta1 gene silencing for treating liver fibrosis. Mol Pharm 2009; 6: 772-779.
5. Sisco M, Kryger ZB, O'Shaughnessy KD, Kim PS, Schultz GS, Ding XZ et al. Antisense inhibition of connective tissue growth factor (CTGF/CCN2) mRNA limits hypertrophic scarring without affecting wound healing in vivo. Wound Repair Regen 2008; 16: 661-673.
6. Gao RP, Brigstock DR. Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function. World J Gastroenterol 2009; 15: 3807-3813.
7. Huang G, Brigstock DR. Regulation of hepatic stellate cells by connective tissue growth factor. Front Biosci 2012; 17: 2495-2497.
8. Li G, Li D, Xie Q, Shi Y, Jiang S, Jin Y. RNA interfering connective tissue growth factor prevents rat hepatic stellate cell activation and extracellular matrix production. J Gene Med 2008; 10: 1039-1047.
9. Zhang D, Wang NY, Yang CB, Fang GX, Liu W, Wen J et al. The clinical value of serum connective tissue growth factor in the assessment of liver fibrosis. Dig Dis Sci 2010; 55: 767-774.
10. George J, Tsutsumi M. siRNA-mediated knockdown of connective tissue growth factor prevents N-nitrosodimethylamine - induced hepatic fibrosis in rats. Gene Ther 2007; 14: 790-803.
11. Li G, Xie Q, Shi Y, Li D, Zhang M, Jiang S et al. Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats. J GeneMed 2006; 8: 889-900.
12. Roderburg C, Urban GW, Bettermann K, Vucur M, Zimmermann H, Schmidt S et al. Micro-RNA profiling reveals a role for miR-29 in human and murine liver fibrosis. Hepatology 2011; 53: 209-218.
13. Maubach G, Lim MC, Chen J, Yang H, Zhuo L. miRNA studies in vitro and in vivo activated hepatic stellate cells. World J Gastroenterol 2011; 17: 2748-2773.
14. Hu T, Fu Q, Chen P, Ma L, Sin O, Guo D. Construction of an artificial MicroRNA expression vector forsimultaneous inhibition of multiple genes in mammalian cells. Int J Mol Sci 2009; 10: 2158-2168.
15. Baek MN, Jung KH, Halder D, Choi MR, Lee BH, Jung MH et al. Artificial microRNAbased neurokinin-1 receptor gene silencing reduces alcohol consumption in mice. Neurosci Lett 2010; 475: 124-128.
16. Fan ZD, Zhang L, Gan XB, Gao XY, Zhu GQ. Artificial microRNA interference targeting AT(1a) receptors in paraventricular nucleus attenuates hypertension in rats. Gene Therapy 2011; 19: 810-817.
17. Suzuki R, Oda Y, Utoguchi N, Maruyama K. Progress in the development of ultrasound-mediated gene delivery systems utilizing nano- and microbubbles. J Control Release 2011; 149: 36-41.
18. Mayer CR, Geis NA, Katus HA, Bekeredjian R. Ultrasound targeted microbubble destruction for drug and gene delivery. Expert Opin Drug Deliv 2008; 5: 1121-1138.
19. Bekeredjian R, Chen S, Frenkel PA, Grayburn PA, Shohet RV. Ultrasound-targeted microbubble destruction can repeatedly direct highly specific plasmid expression to the heart. Circulation 2003; 108: 1022-1026.
20. Kheirolomoom A, Dayton PA, Lum AF, Little E, Paoli EE, Zheng H et al. Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle. J Control Release 2007; 118: 275-284.
21. Vandenbroucke RE, Lentacker I, Demeester J, De Smedt SC, Sanders NN. Ultrasound assisted siRNA delivery using PEG-siPlex loaded microbubbles. J Control Release 2008; 126: 265-273.
22. Yang D, Tan KB, Gao YH, Liu H, Yang WX. Effects of diagnostic ultrasound-targeted microbubble destruction on permeability of normal liver in rats. Ultrasonics 2012; 52: 1065-1071.
23. Mingozzi F, High KA. Immune responses to AAV in clinical trials. Curr Gene Ther 2011; 11: 321-330.
24. Chen SW, Zhang XR, Wang CZ, Chen WZ, Xie WF, Chen YX. RNA interference targeting the platelet-derived growth factor receptor beta subunit ameliorates experimental hepatic fibrosis in rats. Liver Int 2008; 28: 1446-1457.
25. Sawyer GJ, Rela M, Davenport M, Whitehorne M, Zhang X, Fabre JW. Hydrodynamic gene delivery to the liver: Theoretical and practical issues for clinical application. Curr Gene Ther 2009; 9: 128-135.
26. Carson AR, McTiernan CF, Lavery L, Hodnick A, Grata M, Leng X et al. Gene therapy of carcinoma using ultrasound- targeted microbubble destruction. Ultrasound Med Biol 2011; 37: 393-402.
27. Vancraeynest D, Havaux X, Pasquet A, Gerber B, Beauloye C, Rafter P et al. Myocardial injury induced by ultrasound-targeted microbubble destruction: Evidence for the contribution of myocardial ischemia. Ultrasound Med Biol 2009; 35: 672-679.
28. Qiu L, Zhang L, Wang L, Jiang Y, Luo Y, Peng Y et al. Ultrasound-targeted microbubble destruction enhances naked plasmid DNA transfection in rabbit Achilles tendons in vivo. Gene Therapy 2011; 19: 703-710.
29. Liu P, Wang X, Zhou YS, Hua X, Liu Z, Gao Y. Effects of a novel ultrasound contrast agent with long persistence on right ventricular pressure: Comparison with Sonovue. Ultrasonics 2011; 51: 210-214.
30. Liu H, Wang X, Tan KB, Liu P, Zhuo ZX, Liu Z et al. Molecular imaging of vulnerable plaques in rabbits using contrast-enhanced ultrasound targeting to vascular endothelial growth factor receptor-2. J Clin Ultrasound 2011; 39: 83-90.
31. Xu YL, Gao YH, Liu Z, Tan KB, Hua X, Fang ZQ et al. Myocardium-targeted transplantation of mesenchymal stem cells by diagnostic ultrasound-mediated microbubble destruction improves cardiac function in myocardial infarction of New Zealand rabbits. Int J Cardiol 2010; 138: 182-195.
32. Gao Y, Gao S, Zhao B, Zhao Y, Hua X, Tan K et al. Vascular effects of microbubbleenhanced, pulsed, focused ultrasound on liver blood perfusion. Ultrasound Med Biol 2012; 38: 91-98.
33. Tros de Ilarduya C, Sun Y, Dü zgünes N. Gene delivery by lipoplexes and polyplexes. Eur J Pharm Sci 2010; 40: 159-170.
34. Wang W, Liu GJ, Xie XY, Xu ZF, Chen LD, Huang GL et al. Development and evaluation of lipid microbubbles targeted to alpha(v)beta(3)-integrin via biotinavidin bridge. J Microencapsul 2012; 29: 177-184.
35. Lee ES, Shin MO, Yoon S, Moon JO. Resveratrol inhibits dimethylnitrosamineinduced hepatic fibrosis in rats. Arch Pharm Res 2010; 33: 925-932.
36. Gieling RG, Burt AD, Mann DA. Fibrosis and cirrhosis reversibility - molecular mechanisms. Clin Liver Dis 2008; 12: 915-937.
37. Ismail MH, Pinzani M. Reversal of liver fibrosis. Saudi J Gastroenterol 2009; 15: 72-79.
38. Li CH, Piao DM, Xu WX, Yin ZR, Jin JS, Shen ZS. Morphological and serum hyaluronic acid, laminin and type IV collagen changes in dimethylnitrosamineinduced hepatic fibrosis of rats. World J Gastroenterol 2005; 11: 7620-7624.