Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats

Journal of Gene Medicine

Volumn 8, Issue 7, 2006, Pages 889-900

  Li, Guangming ^a   Xie, Qing ^b   Shi, Yi ^c   Li, Dingguo ^a   Zhang, Mingjun ^b   Jiang, Shan ^b   Zhou, Huijuan ^b   Lu, Hanming ^a   Jin, Youxin ^c  

^a XINHUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b SHANGHAI SECOND MEDICAL UNIVERSITY   (China)

^c Shanghai Jiao Tong University Affiliated Sixth People s Hospital   (China)

Author keywords

Connective tissue growth factor; Hepatic fibrosis; Hepatic stellate cell; RNA interference; Small interfering RNA

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; CARBON TETRACHLORIDE; COLLAGEN TYPE 1; COLLAGEN TYPE 3; CONNECTIVE TISSUE GROWTH FACTOR; GENECHEM; HYDROXYPROLINE; LAMININ; PROCOLLAGEN; SMAD2 PROTEIN; SMAD7 PROTEIN; SMALL INTERFERING RNA; SODIUM CHLORIDE; TISSUE INHIBITOR OF METALLOPROTEINASE 1; TRANSFORMING GROWTH FACTOR BETA1;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL ACTIVATION; CELL COUNT; CONTROLLED STUDY; DISEASE CLASSIFICATION; DRUG MECHANISM; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC CODE; HISTOPATHOLOGY; LIVER FIBROSIS; MALE; NONHUMAN; PORTAL VEIN; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN TARGETING; RAT; RAT STRAIN; SATELLITE CELL;

ANIMALS; BASE SEQUENCE; CARBON TETRACHLORIDE; GENE EXPRESSION; GENE SILENCING; IMMEDIATE-EARLY PROTEINS; INJECTIONS, INTRAVENOUS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LIVER; LIVER CIRRHOSIS; MALE; PORTAL VEIN; RATS; RATS, SPRAGUE-DAWLEY; RNA, SMALL INTERFERING; SMAD2 PROTEIN; SMAD7 PROTEIN; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1;

EID: 33746265975     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.894     Document Type: Article

References (35)

1. Shek FW, Benyon RC. How can transforming growth factor beta be targeted usefully to combat liver fibrosis? Eur J Gastroenterol Hepatol 2004; 16: 123-126.
2. mS. Roles of TGF-beta in hepatic fibrosis. Front Biosci 2002; 7: 793-807.
3. Schuppan D, Porov Y. Hepatic fibrosis: from bench to bedside. J Gastroenterol Hepatol 2002; 17(suppl 3): S300-305.
4. Hui AY, Friedman SL. Molecular basis of hepatic fibrosis. Expert Rev Mol Med 2003; 14: 1-23.
5. Paradis V, Dargere D, Bonvoust F, Vidaud M, Segarini P, Bedossa P. Effect and regulation of connective tissue growth factor on hepatic stellate cells. Lab Invest 2002; 82: 767-774.
6. Grotendorst, GR. Connective tissue growth factor: a mediator of TGF-beta action on fibroblasts. Cytokine Growth Factor Rev 1997; 8: 171-179.
7. Leask A, Abraham DJ. The role of connective tissue growth factor, a multifunctional matricellular protein, in fibroblast biology. Biochem Cell Biol 2003; 81: 355-363.
8. Holmes A, Abraham DJ, Chen Y, et al. Constitutive connective tissue growth factor expression in scleroderma fibroblasts is dependent on Sp1. J Biol Chem 2003; 278: 41728-41733.
9. Paradis V, Dargere D, Vidaud M, et al. Expression of connective tissue growth factor in experimental rat and human liver fibrosis. Hepatology 1999; 30: 968-976.
10. Hayashi N, Kakimuma T, Soma Y, et al. Connective tissue growth factor in directly related to liver fibrosis. Hepatogastroenterology 2002; 49: 133-135.
11. Abou-shady M, Friess H, Zimmermann A, et al. Connective tissue growth factor in human liver cirrhosis. Liver 2000; 20: 296-304.
12. Duncan MR, Frazier KS, Abramson S, et al. Connective tissue growth factor mediates transforming growth factor β-induced collagen synthesis: down-regulation by cAMP. FASEB J 1999; 13: 1774-1786.
13. Yokoi H, Mukoyama M, Sugawara A, et al. Role of connective tissue growth factor in fibronectin expression and tubulointerstitial fibrosis. Am J Physiol 2002; 282: F933-942.
14. Wang JF, Olson ME, Ma L, Brigstock DR, Hart DA. Connective tissue growth factor siRNA modulates mRNA levels for a subset of molecules in normal and TGF-beta1-stimulated porcine skin fibroblasts. Wound Repair Regen 2004; 12: 205-216.
15. Yokoi H, Mukoyama M, Nagae T, et al. Reduction in connective tissue growth factor by antisense treatment ameliorates renal tubulointerstitial fibrosis. J Am Soc Nephrol 2004; 15: 1430-1440.
16. Stratton R, Rajkumar, V, Ponticos M, et al. Prostacyclin derivatives prevent the fibrotic response to TGF-beta by inhibiting the Ras/MEK/ERK pathway. FASEB J 2002; 16: 1949-1951.
17. Ivkovic S, Yoon BS, Popoff SN, et al. Connective tissue growth factor coordinates chondrogenesis and angiogenesis during skeletal development. Development 2003; 130: 2779-2791.
18. Blom IF, Goldschmeding R, Leask A. Gene regulation of connective tissue growth factor: new targets for antifibrotic therapy? Matrix Biol 2002; 21: 473-482.
19. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411: 494-498.
20. Song E, Lee SK, Wang J, et al. RNA interference targeting Fas protects mice from fulminant hepatitis. Nat Med 2003; 9: 347-351.
21. Zender L, Hutker S, Liedtke C, et al. Caspase 8 small interfering RNA prevents acute liver failure in mice. Proc Natl Acad Sci U S A 2003; 100: 7797-7802.
22. Sedlaczek N, Jia JD, Bauer M, Herbst H, Ruehl M, Hahn EG. Proliferating bile duct epithelial cells are a major source of connective tissue growth factor in rat biliary fibrosis. Am J Pathol 2001; 158: 1239-1244.
23. Lewis DL, Hagstrom JE, Loomis AG, Wolff JA, Herwijer H. Efficient delivery of siRNA for inhibition of gene expression in postnatal mice. Nat Genet 2002; 32: 107-108.
24. Chen A, Beno DWA, Davis BH. Suppression of stellate cell type I collagen gene expression involves AP-2 transmodulation of nuclear factor-1-dependent gene transcription. J Biol Chem 1996; 271: 25994-25998.
25. Gao R, Ball DK, Perbal B, et al. Connective tissue growth factor induces c-fos gene activation and cell proliferation through p44/42 MAP kinase in primary rat hepatic stellate cells. J Hepatol 2004; 40: 431-438.
26. Poynard T, Bedossa P, Opolon P. Natural history of liver fibrosis progression in patients with chronic hepatitis C. The OBSVIRC, METAVIR, CLINIVIR, and DOSVIRC groups. Lancet 1997; 349: 825-832.
27. Shackel NA, Rockey DC. Intrahepatic gene silencing by RNA interference. Gastroenterology 2004; 126: 356-358.
28. Uchio K, Graham M, Dean NM, et al. Down-regulation of connective tissue growth factor and type I collagen mRNA expression by connective tissue growth factor antisense oligonucleotide during experimental liver fibrosis. Wound Repair Regen 2004; 12: 60-66.
29. Rachfal AW, Brigstock DR. Connective tissue growth factor (CTGF/CCN2) in hepatic fibrosis. Hepatol Res 2003; 26: 1-9.
30. Paradis V, Perlemuter G, Bonvoust F, et al. High glucose and hyperinsulinemia stimulate connective tissue growth factor expression: a potential mechanism involved in progression to fibrosis in nonalcoholic steatohepatitis. Hepatology 2001; 34: 738-744.
31. Letterio JJ, Roberts, AB. Regulation of immune responses by TGF-beta. Annu Rev Immunol 1998; 16: 137-161.
32. Ashcroft GS, Yang X, Glick AB, et al. Mice lacking Smad3 show accelerated wound healing and an impaired local inflammatory response. Nat Cell Biol 1999; 1: 260-266.
33. Soutschek J, Akinc A, Bramlage B, et al. Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs. Nature 2004; 11: 173-178.
34. Greupink R, Bakker HI, Reker-Smit C, et al. Studies on the targeted delivery of the antifibrogenic compound mycophenolic acid to the hepatic stellate cell. J Hepatol 2005; 43: 884-892.
35. Ye Z, Cheng K, Guntaka RV, Mahato RI. Targeted delivery of a triplex-forming oligonucleotide to hepatic stellate cells. Biochemistry 2005; 22: 4466-4476.