메뉴 건너뛰기




Volumn 10, Issue 5, 2009, Pages 2158-2168

Construction of an artificial microRNA expression vector for simultaneous inhibition of multiple genes in mammalian cells

Author keywords

Artificial microRNA; Expression vector; Multiple genes; RNA interference

Indexed keywords

BETA GALACTOSIDASE; ENHANCED GREEN FLUORESCENT PROTEIN; FIREFLY LUCIFERASE; MICRORNA; PRIMER RNA;

EID: 67049118082     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms10052158     Document Type: Article
Times cited : (21)

References (32)
  • 2
    • 8144225486 scopus 로고    scopus 로고
    • MicroRNA genes are transcribed by RNA polymerase II
    • Lee, Y.; Kim, M.; Han, J.; Yeom, K.H.; Lee, S.; Baek, S.H.; Kim, V.N. MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 2004, 23, 4051-4060.
    • (2004) EMBO J , vol.23 , pp. 4051-4060
    • Lee, Y.1    Kim, M.2    Han, J.3    Yeom, K.H.4    Lee, S.5    Baek, S.H.6    Kim, V.N.7
  • 3
    • 9344235449 scopus 로고    scopus 로고
    • Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs
    • Cai, X.; Hagedorn, C.H.; Cullen, B.R., Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 2004, 10, 1957-1966.
    • (2004) RNA , vol.10 , pp. 1957-1966
    • Cai, X.1    Hagedorn, C.H.2    Cullen, B.R.3
  • 4
    • 33344477448 scopus 로고    scopus 로고
    • Genomics of microRNA
    • Kim, V.N.; Nam, J.W. Genomics of microRNA. Trends Genet. 2006, 22, 165-173.
    • (2006) Trends Genet , vol.22 , pp. 165-173
    • Kim, V.N.1    Nam, J.W.2
  • 7
    • 10644234841 scopus 로고    scopus 로고
    • The Drosha-DGCR8 complex in primary microRNA processing
    • Han, J.; Lee, Y.; Yeom, K.H.; Kim, Y.K.; Jin, H.; Kim, V.N., The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev. 2004, 18, 3016-3027.
    • (2004) Genes Dev , vol.18 , pp. 3016-3027
    • Han, J.1    Lee, Y.2    Yeom, K.H.3    Kim, Y.K.4    Jin, H.5    Kim, V.N.6
  • 9
    • 0347361541 scopus 로고    scopus 로고
    • Exportin-5 mediates the nuclear export of premicroRNAs and short hairpin RNAs
    • Yi, R.; Qin, Y.; Macara, I.G.; Cullen, B.R. Exportin-5 mediates the nuclear export of premicroRNAs and short hairpin RNAs. Genes Dev. 2003, 17, 3011-3016.
    • (2003) Genes Dev , vol.17 , pp. 3011-3016
    • Yi, R.1    Qin, Y.2    Macara, I.G.3    Cullen, B.R.4
  • 10
    • 0035800521 scopus 로고    scopus 로고
    • A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    • Hutvagner, G.; McLachlan, J.; Pasquinelli, A.E.; Balint, E.; Tuschl, T.; Zamore, P.D. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 2001, 293, 834-838.
    • (2001) Science , vol.293 , pp. 834-838
    • Hutvagner, G.1    McLachlan, J.2    Pasquinelli, A.E.3    Balint, E.4    Tuschl, T.5    Zamore, P.D.6
  • 11
    • 0035887005 scopus 로고    scopus 로고
    • Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
    • Ketting, R.F.; Fischer, S.E.; Bernstein, E.; Sijen, T.; Hannon, G.J.; Plasterk, R.H. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev. 2001, 15, 2654-2659.
    • (2001) Genes Dev , vol.15 , pp. 2654-2659
    • Ketting, R.F.1    Fischer, S.E.2    Bernstein, E.3    Sijen, T.4    Hannon, G.J.5    Plasterk, R.H.6
  • 12
    • 27744537851 scopus 로고    scopus 로고
    • Human RISC couples microRNA biogenesis and posttranscriptional gene silencing
    • Gregory, R.I.; Chendrimada, T.P.; Cooch, N.; Shiekhattar, R. Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. Cell 2005, 123, 631-640.
    • (2005) Cell , vol.123 , pp. 631-640
    • Gregory, R.I.1    Chendrimada, T.P.2    Cooch, N.3    Shiekhattar, R.4
  • 13
    • 0036294527 scopus 로고    scopus 로고
    • Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells
    • Zeng, Y.; Wagner, E.J.; Cullen, B.R. Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol. Cell 2002, 9, 1327-1333.
    • (2002) Mol. Cell , vol.9 , pp. 1327-1333
    • Zeng, Y.1    Wagner, E.J.2    Cullen, B.R.3
  • 14
    • 33747603277 scopus 로고    scopus 로고
    • Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown
    • Sun, D.; Melegari, M.; Sridhar, S.; Rogler, C.E.; Zhu, L. Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown. Biotechniques 2006, 41, 59-63.
    • (2006) Biotechniques , vol.41 , pp. 59-63
    • Sun, D.1    Melegari, M.2    Sridhar, S.3    Rogler, C.E.4    Zhu, L.5
  • 16
    • 55049131163 scopus 로고    scopus 로고
    • A tightly regulated Pol III promoter for synthesis of miRNA genes in tandem
    • Zhou, H.; Huang, C.; Xia, X.G. A tightly regulated Pol III promoter for synthesis of miRNA genes in tandem. Biochim. Biophys. Acta 2008, 1779, 773-779.
    • (2008) Biochim. Biophys. Acta , vol.1779 , pp. 773-779
    • Zhou, H.1    Huang, C.2    Xia, X.G.3
  • 17
    • 33751113540 scopus 로고    scopus 로고
    • Design of expression vectors for RNA interference based on miRNAs and RNA splicing
    • Du, G.; Yonekubo, J.; Zeng, Y.; Osisami, M.; Frohman, M.A. Design of expression vectors for RNA interference based on miRNAs and RNA splicing. FEBS J. 2006, 273, 5421-5427.
    • (2006) FEBS J , vol.273 , pp. 5421-5427
    • Du, G.1    Yonekubo, J.2    Zeng, Y.3    Osisami, M.4    Frohman, M.A.5
  • 18
    • 28644450043 scopus 로고    scopus 로고
    • An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi
    • Zhou, H.; Xia, X.G.; Xu, Z. An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi. Nucleic Acids Res. 2005, 33, e62.
    • (2005) Nucleic Acids Res , vol.33
    • Zhou, H.1    Xia, X.G.2    Xu, Z.3
  • 19
    • 11844297347 scopus 로고    scopus 로고
    • Use of RNA polymerase II to transcribe artificial microRNAs
    • Zeng, Y.; Cai, X.; Cullen, B.R. Use of RNA polymerase II to transcribe artificial microRNAs. Methods Enzymol. 2005, 392, 371-380.
    • (2005) Methods Enzymol , vol.392 , pp. 371-380
    • Zeng, Y.1    Cai, X.2    Cullen, B.R.3
  • 20
    • 36248979862 scopus 로고    scopus 로고
    • Primer extension-based method for the generation of a siRNA/miRNA expression vector
    • Gou, D.; Zhang, H.; Baviskar, P.S.; Liu, L. Primer extension-based method for the generation of a siRNA/miRNA expression vector. Physiol. Genomics 2007, 31, 554-562.
    • (2007) Physiol. Genomics , vol.31 , pp. 554-562
    • Gou, D.1    Zhang, H.2    Baviskar, P.S.3    Liu, L.4
  • 22
    • 33747754760 scopus 로고    scopus 로고
    • Lessons from Nature: MicroRNA-based shRNA libraries
    • Chang, K.; Elledge, S.J.; Hannon, G.J. Lessons from Nature: microRNA-based shRNA libraries. Nat. Methods 2006, 3, 707-714.
    • (2006) Nat. Methods , vol.3 , pp. 707-714
    • Chang, K.1    Elledge, S.J.2    Hannon, G.J.3
  • 24
    • 30644463822 scopus 로고    scopus 로고
    • Design and cloning strategies for constructing shRNA expression vectors
    • McIntyre, G.J.; Fanning, G.C. Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnol. 2006, 6, 1.
    • (2006) BMC Biotechnol , vol.6 , pp. 1
    • McIntyre, G.J.1    Fanning, G.C.2
  • 26
    • 33947728394 scopus 로고    scopus 로고
    • RNA stem-loops: To be or not to be cleaved by RNAse III
    • Ritchie, W.; Legendre, M.; Gautheret, D. RNA stem-loops: to be or not to be cleaved by RNAse III. RNA 2007, 13, 457-462.
    • (2007) RNA , vol.13 , pp. 457-462
    • Ritchie, W.1    Legendre, M.2    Gautheret, D.3
  • 27
    • 23044502585 scopus 로고    scopus 로고
    • Efficient processing of primary microRNA hairpins by Drosha requires flanking nonstructured RNA sequences
    • Zeng, Y.; Cullen, B.R. Efficient processing of primary microRNA hairpins by Drosha requires flanking nonstructured RNA sequences. J. Biol. Chem. 2005, 280, 27595-27603.
    • (2005) J. Biol. Chem , vol.280 , pp. 27595-27603
    • Zeng, Y.1    Cullen, B.R.2
  • 29
    • 0346727524 scopus 로고    scopus 로고
    • MicroRNAs modulate hematopoietic lineage differentiation
    • Chen, C.Z.; Li, L.; Lodish, H.F.; Bartel, D.P. MicroRNAs modulate hematopoietic lineage differentiation. Science 2004, 303, 83-86.
    • (2004) Science , vol.303 , pp. 83-86
    • Chen, C.Z.1    Li, L.2    Lodish, H.F.3    Bartel, D.P.4
  • 30
    • 10944254152 scopus 로고    scopus 로고
    • Transcription and processing of human microRNA precursors
    • Cullen, B.R. Transcription and processing of human microRNA precursors. Mol. Cell 2004, 16, 861-865.
    • (2004) Mol. Cell , vol.16 , pp. 861-865
    • Cullen, B.R.1
  • 31
    • 20444365531 scopus 로고    scopus 로고
    • Specific inhibition of HIV-1 replication by short hairpin RNAs targeting human cyclin T1 without inducing apoptosis
    • Li, Z.; Xiong, Y.; Peng, Y.; Pan, J.; Chen, Y.; Wu, X.; Hussain, S.; Tien, P.; Guo, D. Specific inhibition of HIV-1 replication by short hairpin RNAs targeting human cyclin T1 without inducing apoptosis. FEBS Lett. 2005, 579, 3100-3106.
    • (2005) FEBS Lett , vol.579 , pp. 3100-3106
    • Li, Z.1    Xiong, Y.2    Peng, Y.3    Pan, J.4    Chen, Y.5    Wu, X.6    Hussain, S.7    Tien, P.8    Guo, D.9
  • 32
    • 59149104555 scopus 로고    scopus 로고
    • Generation of a stable mammalian cell line for simultaneous expression of multiple genes by using 2A peptide-based lentiviral vector
    • Hu, T.; Fu, Q.; Chen, P.; Zhang, K.; Guo, D. Generation of a stable mammalian cell line for simultaneous expression of multiple genes by using 2A peptide-based lentiviral vector. Biotechnol. Lett. 2009, 31, 353-359.
    • (2009) Biotechnol. Lett , vol.31 , pp. 353-359
    • Hu, T.1    Fu, Q.2    Chen, P.3    Zhang, K.4    Guo, D.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.