Stimuli responsive elastin-like polypeptides and applications in medicine and biotechnology

Journal of Biomaterials Science, Polymer Edition

Volumn 25, Issue 2, 2014, Pages 101-120

  Shang, Yuezai ^a,b   Yan, Yufang ^a,b   Hou, Xin ^a,b  

^a TIANJIN KEY LABORATORY OF COMPOSITE AND FUNCTIONAL MATERIALS   (China)

^b TIANJIN UNIVERSITY   (China)

Author keywords

drug delivery; elastin like polypeptides; pentapeptide; protein purification; stimuli responsive biopolymers; tissue engineering

Indexed keywords

BIOLOGICAL APPLICATIONS; ELASTIN-LIKE POLYPEPTIDES; FUSION PROTEINS; INVERSE TEMPERATURE TRANSITIONS; PENTAPEPTIDE; PROTEIN PURIFICATION; STIMULI RESPONSIVE BIOPOLYMERS; STIMULI-RESPONSIVE;

BIOSYNTHESIS; BIOTECHNOLOGY; DRUG DELIVERY; PROTEINS; PURIFICATION; TISSUE ENGINEERING;

BIOMOLECULES;

BIOPOLYMER; ELASTIN LIKE POLYPEPTIDE; HYBRID PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AQUEOUS SOLUTION; ARTICLE; BIOCOMPATIBILITY; BIODEGRADABILITY; BIOTECHNOLOGY; CROSS LINKING; DRUG DELIVERY SYSTEM; GENETIC ENGINEERING; HYDROGEL; HYDROPHOBICITY; MOLECULAR WEIGHT; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN PURIFICATION; SYNTHESIS; THERMODYNAMICS; TISSUE ENGINEERING; TRANSITION TEMPERATURE;

BIOCOMPATIBLE MATERIALS; BIOTECHNOLOGY; ELASTIN; HUMANS; MEDICINE; PEPTIDES;

EID: 84889883948     PISSN: 09205063     EISSN: 15685624     Source Type: Journal    
DOI: 10.1080/09205063.2013.841073     Document Type: Article

References (113)

1. Urry DW, Pattanaik A, Xu J, Woods TC, McPherson DT, Parker TM. Elastic protein-based polymers in soft tissue augmentation and generation. J. Biomater. Sci., Polym. Ed. 1998;9:1015-1048. (Pubitemid 28468397)
2. Chang DC, Wang XL, Hou X, Yao KD. Application of elastin in biomedical materials. J. Biomed. Eng. 2008;25:1454-1457.
3. Keeley FW, Bellingham CM, Woodhouse KA. Elastin as a self-organizing biomaterial: use of recombinantly expressed human elastin polypeptides as a model for investigations of structure and self-assembly of elastin. Philos. Trans. R. Soc. London, Ser. B., Biol. Sci. 2002;357:185-189. (Pubitemid 34276209)
4. Bellingham CM, Lillie MA, Gosline JM, Wright GM, Starcher BC, Bailey AJ, Woodhouse KA, Keeley FW. Recombinant human elastin polypeptides self-assemble into biomaterials with elastin-like properties. Biopolymers. 2003;70:445-455. (Pubitemid 37531828)
5. Daamen WF, Veerkampa JH, van Hestb JCM, van Kuppevelta TH. Elastin as a biomaterial for tissue engineering. Biomaterials. 2007;28:4378-4398. (Pubitemid 47212414)
6. Wang XL, Gan TT, Zhang M, Hou X, Yao KD. Synthesis of elastin graft copolymer via ATRP and its properties. Chem. J. Chin. Univ. 2007;28:789-793. (Pubitemid 46923082)
7. Wang XL, Chang DC, Zhang M, Hou X, Yao KD. Modification of elastin via thioiniferters. Acta Chim. Sinica. 2007;65:749-754.
8. Annabi N, Mithieux SM, Boughton EA, Ruys AJ, Weiss AS, Dehghani F. Synthesis of highly porous crosslinked elastin hydrogels and their interaction with fibroblasts in vitro. Biomaterials. 2009;30:4550-4557.
9. Almine JF, Bax DV, Mithieux SM, Nivison-Smith L, Rnjak J, Waterhouse A, Wise SG, Weiss AS. Elastin-based materials. Chem. Soc. Rev. 2010;39:3371-3379.
10. Rodriguez-Cabello JC, Prieto S, Reguera J, Arias FJ, Ribeiro A. Biofunctional design of elastin-like polymers for advanced applications in nanobiotechnology. J. Biomater. Sci., Polym. Ed. 2007;18:269-286. (Pubitemid 46403136)
11. Urry DW. Physical chemistry of biological free energy transduction as demonstrated by elastic protein-based polymers. J. Phys. Chem. B. 1997;101:11007-11028. (Pubitemid 127627954)
12. Li B, Alonso DOV, Bennion BJ, Daggett V. Hydrophobic hydration is an important source of elasticity in elastin-based biopolymers. J. Am. Chem. Soc. 2001;123:11991-11998. (Pubitemid 33135030)
13. Li B, Alonso DOV, Daggett V. The molecular basis for the inverse temperature transition of elastin. J. Mol. Biol. 2001;305:581-592. (Pubitemid 33032863)
14. Schild HG, Tirrell DA. Microcalorimetric detection of lower critical solution temperatures in aqueous polymer solutions. J. Phys. Chem. 1990;94:4352-4356.
15. Meyer DE, Chilkoti A. Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides. Biomacromolecules. 2004;5:846-851. (Pubitemid 38702255)
16. Ghoorchian A, Holland NB. Molecular architecture influences the thermally induced aggregation behavior of elastin-like polypeptides. Biomacromolecules. 2011;12:4022-4029.
17. Zhang Y, Trabbic-Carlson K, Albertorio F, Chilkoti A, Cremer PS. Aqueous two-phase system formation kinetics for elastin-like polypeptides of varying chain length. Biomacromolecules. 2006;7:2192-2199. (Pubitemid 44103360)
18. Cho Y, Zhang Y, Christensen T, Sagle LB, Chilkoti A, Cremer PS. Effects of hofmeister anions on the phase transition temperature of elastin-like polypeptides. J. Phys. Chem. B. 2008;112:13765-13771.
19. Golonka M, Bulwan M, Nowakowska M, Testera AM, Rodríguez-Cabello JC, Zapotoczny S. Thermoresponsive multilayer films based on ionic elastin-like recombinamers. Soft Matter. 2011;7:9402-9409.
20. Costa RR, Custodio CA, Testera AM, Arias FJ, Rodriguez-Cabello JC, Alves NM, Mano JF. Stimuli-responsive thin coatings using elastin-like polymers for biomedical applications. Adv. Funct. Mater. 2009;19:3210-3218.
21. MacKay JA, Callahan DJ, FitzGerald KN, Chilkoti A. Quantitative model of the phase behavior of recombinant pH-responsive elastin-like polypeptides. Biomacromolecules. 2010;11:2873-2879.
22. Alvarez-Rodriguez R, Arias FJ, Santos M, Testera AM, Rodriguez-Cabello JC. Gold tailored photosensitive elastin-like polymer: synthesis of temperature, pH and UV-vis sensitive probes. Macromol. Rapid Commun. 2010;31:568-573.
23. Urry DW, Luan CH, Peng SQ. Molecular biophysics of elastin structure, function and pathology. Ciba Found. Symp. 1995;192:4-22.
24. Dybal J, Schmidt P, Kurková D, Kříž J. Temperature induced conformational transitions of elastin-like polypentapeptides studied by Raman and NMR spectroscopy. Spectroscopy. 2002;16:251-255. (Pubitemid 36124841)
25. Gross PC, Possart W, Zeppezauer M. An alternative structure model for the polypentapeptide in elastin. J. Biosci. 2003;58:873-878. (Pubitemid 38069723)
26. Serrano V, Liu W, Franzen S. An infrared spectroscopic study of the conformational transition of elastin-like polypeptides. Biophys. J. 2007;93:2429-2453.
27. Urry DW, Parker TM. Mechanics of elastin: Molecular mechanism of biological elasticity and its relationship to contraction. J. Muscle Res. Cell Motil. 2002;23:543-559. (Pubitemid 36583671)
28. Urry DW, Hugel T, Seitz M. Elastin: a representative ideal protein elastomer. R. Soc. 2002;357:169-184. (Pubitemid 34276208)
29. Meyer DE, Chilkoti A. Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system. Biomacromolecules. 2002;3:357-367. (Pubitemid 34257157)
30. Urry DW, Luan CH, Parker TM, Gowda DC, Prasad KU, Reid MC, Safavy A. Temperature of polypeptide inverse temperature transition depends on mean residue hydrophobicity. J. Am. Chem. Soc. 1991;113:4346-4348.
31. Urry DW. Physical chemistry of biological free energy transduction as demonstrated by elastic protein-based polymers. J. Phys. Chem. B. 1997;101:11007-11028. (Pubitemid 127627954)
32. Girotti A, Reguera J, Arias FJ, Alonso M, Testera AM, Rodriguez-Cabello JC. Influence of the molecular weight on the inverse temperature transition of a model genetically engineered elastin-like pH-responsive polymer. Macromolecules. 2004;37:3396-3400.
33. Reguera J, Urry DW, Parker TM, McPherson DT, Rodriguez-Cabello JC. Effect of NaCl on the exothermic and endothermic components of the inverse temperature transition of a model elastin-like polymer. Biomacromolecules. 2007;8:354-358. (Pubitemid 46323247)
34. Ribeiro A, Arias FJ, Reguera J, Alonso M, Rodriguez-Cabello JC. Influence of the aminoacid sequence on the inverse temperature transition of elastin-like polymers. Biophys. J. 2009;97:312-320.
35. McDaniel JR, Radford DC, Chilkoti A. A unified model for de novo design of elastin-like polypeptides with tunable inverse transition temperatures. Biomacromolecules. 2013;14:2866-2872.
36. Ghandehari H, Cappello J. Genetic engineering of protein-based polymers: potential in controlled drug delivery. Pharm. Res. 1998;15:813-815. (Pubitemid 28307819)
37. Chow DC, Dreher MR, Trabbic-Carlson K, Chilkoti A. Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli. Biotechnol. Progr. 2006;22:638-646. (Pubitemid 43877431)
38. Meyer DE, Chilkoti A. Purification of recombinant proteins by fusion with thermallyresponsive polypeptides. Nat. Biotechnol. 1999;17:1112-1115. (Pubitemid 29533553)
39. Chow D, Nunalee ML, Lim DW, Simnick AJ, Chilkoti A. Peptide-based biopolymers in biomedicine and biotechnology. Mater. Sci. Eng., R. 2008;62:125-155.
40. Urry DW, Parker TM, Reid MC, Gowda DC. Biocompatibility of the bioelastic materials, poly(GVGVP) and its γ-irradiation cross-linked matrix: summary of generic biological test results. J. Bioact. Compat. Polym. 1991;6:263-282.
41. Simnick AJ, Lim DO, Chow D, Chilkoti A. Biomedical and biotechnological applications of elastin-like polypeptides. Polym. Rev. 2007;47:121-154.
42. Liu W, Mackay JA, Dreher MR, Chen M, McDaniel JR, Simnick AJ, Callahan DJ, Zalutsky MR, Chilkoti A. Injectable intratumoral depot of thermally responsive polypeptide- radionuclide conjugates delays tumor progression in a mouse model. J. Control. Release. 2010;144:2-9.
43. Nettles DL, Chilkoti A, Setton LA. Applications of elastin-like polypeptides in tissue engineering. Adv. Drug Delivery Rev. 2010;62:1479-1485.
44. Martino M, Tamburro AM. Chemical synthesis of cross-linked poly(KGGVG), an elastinlike biopolymer. Biopolymers. 2001;59:29-37. (Pubitemid 32531784)
45. Zheng JS, Chang HN, Wang FL, Liu L. Fmoc synthesis of peptide thioesters without postchain- assembly manipulation. J. Am. Chem. Soc. 2011;133:11080- 11083.
46. Mende F, Seitz O. 9-Fluorenylmethoxycarbonyl-based solid-phase synthesis of peptide α-thioesters. Angew. Chem. Int. Ed. 2011;50:1232-1240.
47. Martino M, Coviello A, Tamburro AM. Synthesis and structural characterization of poly (LGGVG), an elastin-like polypeptide. Int. J. Biol. Macromol. 2000;27:59-64. (Pubitemid 30110489)
48. Martino M, Perri T, Tamburro AM. Elastin-based biopolymers: chemical synthesis and structural characterization of linear and cross-linked poly(Orn-GlyGlyOrnGly). Biomacromolecules. 2002;3:297-304. (Pubitemid 34257149)
49. Pechar M, Brus J, Kostka L, Konak C, Urbanova M, Slouf M. Thermoresponsive selfassembly of short elastin-like polypentapeptides and their poly(ethylene glycol) derivatives. Macromol. Biosci. 2007;7:56-69. (Pubitemid 46191960)
50. Betre H, Setton LA, Meyer DE, Chilkoti A. Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair. Biomacromolecules. 2002;3:910-916.
51. McDaniel JR, MacKay JA, Quiroz FG, Chilkoti A. Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes. Biomacromolecules. 2010;11:944-952.
52. Amiram M, Quiroz FG, Callahan DJ, Chilkoti A. A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers. Nat. Mater. 2011;10:141-148.
53. Nagapudi K, Brinkman WT, Thomas BS, Park JQ, Srinivasarao M, Wright E, Conticello VP, Chaikof EL. Viscoelastic and mechanical behavior of recombinant protein elastomers. Biomaterials. 2005;26:4695-4706. (Pubitemid 40343018)
54. Wu X, Sallach RE, Caves JM, Conticello VP, Chaikof EL. Deformation responses of a physically cross-linked high molecular weight elastin-like protein polymer. Biomacromolecules. 2008;9:1787-1794.
55. Hassouneh W, Fischer K, MacEwan SR, Branscheid R, Fu CL, Liu R, Schmidt M, Chilkoti A. Unexpected multivalent display of proteins by temperature triggered selfassembly of elastin-like polypeptide block copolymers. Biomacromolecules. 2012;13: 1598-1605.
56. MacEwan SR, Chilkoti A. Elastin-like polypeptides: biomedical applications of tunable biopolymers. Pept. Sci. 2010;94:60-77.
57. Chilkoti A, Christensen T, MacKay JA. Stimulus responsive elastin biopolymers: applications in medicine and biotechnology. Curr. Opin. Chem. Biol. 2006;10:652-657. (Pubitemid 44821899)
58. Floss DM, Schallau K, Rose-John S, Conrad U, Scheller J. Elastin-like polypeptides revolutionize recombinant protein expression and their biomedical application. Trends Biotechnol. 2010;28:37-45.
59. Trabbic-Carlson K, Meyer DE, Liu L, Piervincenzi R, Nath N, LaBean T, Chilkoti A. Effect of protein fusion on the transition temperature of an environmentally responsive elastin- like polypeptide: a role for surface hydrop-hobicity? Protein Eng. Des. Sel. 2004;17:57-66. (Pubitemid 38173169)
60. Christensen T, Hassouneh W, Trabbic-Carlson K, Chilkoti A. Predicting transition temperatures of elastin-like polypeptide fusion proteins. Biomacromolecules. 2013;14:1514-1519.
61. Bellucci JJ, Amiram M, Bhattacharyya J, McCafferty D, Chilkoti A. Three-in-one chromatography- free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides. Angew. Chem. Int. Ed. Engl. 2013;52:3703-3708.
62. Trabbic-Carlson K, Liu L, Kim B, Chilkoti A. Expression and purification of recombinant proteins from Escherichia coli: comparison of an elastin-like polypeptide fusion with an oligohistidine fusion. Protein Sci. 2004;13:3274-3284. (Pubitemid 39557797)
63. Lan DM, Huang GR, Shao HW, Zhang LC, Ma LX, Chen SW, Xu AL. An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases. Anal. Biochem. 2011;415:200-202.
64. Conley AJ, Joensuu JJ, Richman A, Menassa R. Protein body-inducing fusions for highlevel production and purification of recombinant proteins in plants. Plant Biotechnol. J. 2011;9:419-433.
65. Christensen T, Trabbic-Carlson K, Liu W, Chilkoti A. Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling. Anal. Biochem. 2007;360:166-168. (Pubitemid 44894337)
66. Ge X, Filipe CD. Simultaneous phase transition of ELP tagged molecules and free ELP: an efficient and reversible capture system. Biomacromolecules. 2006;7:2475-2478. (Pubitemid 44530483)
67. Lim DW, Trabbic-Carlson K, MacKay JA, Chilkoti A. Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides. Biomacromolecules. 2007;8:1417-1424. (Pubitemid 46865112)
68. Madan B, Chaudhary G, Cramer SM, Chen W. ELP-z and ELP-zz capturing scaffolds for the purification of immunoglobulins by affinity precipitation. J. Biotechnol. 2013;163:10-14.
69. Yu SQ, Liu YH. Expression and one-step purification of a β-galactosidase by fusion with elastin-like polypeptides. Process Biochem. 2012;47:1108-1114.
70. Srokowski EM, Blit PH, McClung WG, Brash JL, Santerre JP, Woodhouse KA. Platelet adhesion and fibrinogen accretion on a family of elastin-like polypeptides. J. Biomater. Sci., Polym. Ed. 2011;22:41-57.
71. Bidwell GL, III, Raucher D. Cell penetrating elastin-like polypeptides for therapeutic peptide delivery. Adv. Drug Delivery Rev. 2010;62:1486-1496.
72. McDaniel JR, Callahan DJ, Chilkoti A. Adv. drug delivery to solid tumors by elastin-like polypeptides. Adv. Drug Delivery Rev. 2010;62:1456-1467.
73. Manzoor AA, Lindner LH, Landon CD. Overcoming limitations in nanoparticle drug delivery: triggered, intravascular release to improve drug penetration into tumors. Cancer Res. 2012;72:5566-5575.
74. Fujita Y, Mie M, Kobatake E. Construction of nanoscale protein particle using temperaturesensitive elastin-like peptide and polyaspartic acid chain. Biomaterials. 2009;30:3450-3457.
75. Ghoorchian A, Cole JT, Holland NB. Thermoreversible micelle formation using a threearmed star elastin-like polypeptide. Macromolecules. 2010;43:4340-4345.
76. McDaniel JR, Bhattacharyya J, Vargo KB, Hassouneh W, Hammer DA, Chilkoti A. Selfassembly of thermally responsive nanoparticles of a genetically encoded peptide polymer by drug conjugation. Angew. Chem. Int. Ed. Engl. 2013;52:1683-1687.
77. MacKay JA, Chen MN, McDaniel JR, Liu WG, Simnick AJ, Chilkoti A. Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumours after a single injection. Nat. Mater. 2009;8:993-999.
78. Meyer DE, Shin BC, Kong GA, Dewhirst MW, Chilkoti A. Drug targeting using thermally responsive polymers and local hyperthermia. J. Control. Release. 2001;74:213-224. (Pubitemid 32727623)
79. McDaniel JR, MacEwan SR, Dewhirst M, Chilkoti A. Doxorubicin-conjugated chimeric polypeptide nanoparticles that respond to mild hyperthermia. J. Control. Release. 2013;159:362-367.
80. Dreher MR, Liu W, Michelich CR, Dewhirst MW, Chilkoti A. Thermal cycling enhances the accumulation of a temperature-sensitive biopolymer in solid tumors. Cancer Res. 2007;67:4418-4424. (Pubitemid 46815091)
81. Moktan S, Raucher D. Anticancer activity of proapoptotic peptides is highly improved by thermal targeting using elastin-like polypeptides. Int. J. Pept. Res. Ther. 2012;18:227-237.
82. Amirama M, Luginbuhl KM, Li X, Feinglos MN, Chilkoti A. Injectable protease-operated depots of glucagon-like peptide-1 provide extended and tunable glucose control. Proc. Nat. Acad. Sci. U.S.A. 2013;110:2792-2797.
83. Dreher MR, Simnick AJ, Fischer K, Smith RJ, Patel A, Schmidt M, Chilkoti A. Temperature triggered self-assembly of polypeptides into multivalent spherical micelles. J. Am. Chem. Soc. 2008;130:687-694. (Pubitemid 351455582)
84. Lutolf MMP, Hubbell JJA. Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat. Biotechnol. 2005;23:47-55.
85. Srokowski EM, Woodhouse KA. Development and characterisation of novel cross-linked bio-elastomeric materials. J. Biomater. Sci., Polym. Ed. 2008;19:785-799. (Pubitemid 351820783)
86. Nagaoka M, Jiang HL, HoshibaI T, Akaike T, Cho CS. Application of recombinant fusion proteins for tissue engineering. Ann. Biomed. Eng. 2010;38:683-693.
87. Nettles DL, Kitaoka K, Hanson NA, Flahiff CM, Mata BA, Hsu EW, Chilkoti A, Setton LA. In situ crosslinking elastin-like polypeptide gels for application to articular cartilage repair in a goat osteochondral defect model. Tissue Eng. Part A. 2008;14:1133-1140. (Pubitemid 351931505)
88. Betre H, Ong SR, Guilak F, Chilkoti A, Fermor B, Setton LA. Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide. Biomaterials. 2006;27:91-99. (Pubitemid 41225372)
89. Martinez-Osorio H, Juarez-Campo M, Diebold Y, Girotti A, Alonso M, Arias FJ, Rodriguez-Cabello JC, Garcia-Vazquez C, Calonge M. Genetically engineered elastin-like polymer as a substratum to culture cells from the ocular surface. Curr. Eye Res. 2009;34:48-56.
90. Wu X, Sallach R, Haller CA, Caves JA, Nagapudi K, Conticello VP, Levenston ME, Chaikof EL. Alterations in physical cross-linking modulate mechanical properties of two-phase protein polymer networks. Biomacromolecules. 2005;6:3037-3044. (Pubitemid 41764059)
91. Ma M, Kuang Y, Gao Y, Zhang Y, Gao P, Xu B. Aromatic-aromatic interactions induce the self-assembly of pentapeptidic derivatives in water to form nanofibers and supramolecular hydrogels. J. Am. Chem. Soc. 2010;132:2719-2728.
92. Sallach RE, Cui W, Wen J, Martinez A, Conticello VP, Chaikof EL. Elastin-mimetic protein polymers capable of physical and chemical crosslinking. Biomaterials. 2009;30:409-422.
93. Sallach RE, Cui W, Balderrama F, Martinez AW, Wen J, Haller CA, Taylor JV, Wright ER, Long RC, Jr, Chaikof EL. Long-term biostability of self-assembling protein polymers in the absence of covalent crosslinking. Biomaterials. 2010;31:779-791.
94. Krishna OD, Wiss KT, Luo T, Pochan DJ, Theato P, Kiick KL. Morphological transformations in a dually thermoresponsive coil-rod-coil bioconjugate. Soft Matter. 2012;8:3832-3840.
95. Lee J, Macosko CW, Urry DW. Swelling behavior of γ-irradiation cross-linked elastomeric polypentapeptide-based hydrogels. Macromolecules. 2001;34:4114-4123. (Pubitemid 34216857)
96. Lee J, Macosko CW, Urry DW. Mechanical properties of cross-linked synthetic elastomeric polypentapeptides. Macromolecules. 2001;34:5968-5974. (Pubitemid 34217207)
97. McMillan RA, Conticello VP. Synthesis and characterization of elastin-mimetic protein gels derived from a well-defined polypeptide precursor. Macromolecules. 2000;33:4809-4821.
98. Raphel J, Parisi-Amon A, Heilshorn SC. Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings. J. Mater. Chem. 2012;22:19429-19437.
99. Hrabchak C, Rouleau J, Moss I, Woodhouse K, Akens M, Bellinghame C, Keeley F, Dennis M, Yee A. Assessment of biocompatibility and initial evaluation of genipin cross-linked elastin-like polypeptides in the treatment of an osteochondral knee defect in rabbits. Acta Biomater. 2010;6:2108-2115.
100. Lim DW, Nettles DL, Setton LA, Chilkoti A. In situ cross-linking of elastin-like polypeptide block copolymers for tissue repair. Biomacromolecules. 2008;9:222-230. (Pubitemid 351201602)
101. Lim DW, Nettles DL, Setton LA, Chilkoti A. Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution. Biomacromolecules. 2007;8:1463-1470. (Pubitemid 46865117)
102. Trabbic-Carlson K, Setton LA, Chilkoti A. Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides. Biomacromolecules. 2003;4:572-580. (Pubitemid 36701226)
103. Nettles DL, Haider MA, Chilkoti A, Setton LA. Neural network analysis identifies scaffold properties necessary for in vitro chondrogenesis in elastin-like polypeptide biopolymer scaffolds. Tissue Eng. Part A. 2010;16:11-20.
104. Krishna UM, Martinez AW, Caves JM, Chaikof EL. Hydrazone self-crosslinking of multiphase elastin-like block copolymer networks. Acta Biomater. 2012;8:988-997.
105. Xu DH, Asai D, Chilkoti A, Craig SL. Rheological properties of cysteine-containing elastin- like polypeptide solutions and hydrogels. Biomacromolecules. 2012;13:2315-2321.
106. Girotti A, Reguera J, Rodriguez-Cabello JC, Arias FJ, Alonso M, Testera AM. Design and bioproduction of a recombinant multi(bio)functional elastin-like protein polymer containing cell adhesion sequences for tissue engineering purposes. J. Mater. Sci., Mater. Med. 2004;15:479-484. (Pubitemid 39012166)
107. Tejeda-Montes E, Smith KH, Poch M, López-Bosque MJ, Martín L, Alonso M, Engel E, Mata A. Engineering membrane scaffolds with both physical and biomolecular signaling. Acta Biomater. 2012;8:998-1009.
108. McHale KM, Setton LA, Chilkoti A. Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair. Tissue Eng. 2005;11:1768-1779. (Pubitemid 43120188)
109. Bozzini S, Giuliano L, Altomare L, Bandiera A, Conconi MT, Fare S, Tanzi MC. Enzymatic cross-linking of human recombinant elastin (HELP) as biomimetic approach in vascular tissue engineering. J. Mater. Sci., Mater. Med. 2011;22:2641-2650.
110. Lorand L, Graham RM. Transglutaminases: crosslinking enzymes with pleiotropic functions. Nat. Rev. Mol. Cell. Biol. 2003;4:140-156. (Pubitemid 36172696)
111. Summey BT, Graff RD, Lai TS, Greenberg CS, Lee GM. Tissue transglutaminase localization and activity regulation in the extracellular matrix of articular cartilage. J. Orthopaedic Res. 2002;20:76-82. (Pubitemid 34071424)
112. Sperinde JJ, Griffith LG. Synthesis and characterization of enzymatically-cross-linked poly (ethylene glycol) hydrogels. Macromolecules. 1997;30:5255-5264. (Pubitemid 127564780)
113. Shah M, Hsueh PY, Sun GY, Chang HY, Janib SM, MacKay JA. Biodegradation of elastin- like polypeptide nanoparticles. Protein Sci. 2012;21:743-750.