Expression and one-step purification of a β-galactosidase by fusion with elastin-like polypetides

Process Biochemistry

Volumn 47, Issue 7, 2012, Pages 1108-1114

  Yu, Shi Qin ^a   Liu, Yu Huan ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Galactosidase; Elastin like polypeptide (ELP); Halophilic enzyme; One step purification

Indexed keywords

AMMONIUM SULFATE; ELASTIN-LIKE POLYPEPTIDES; FUSION PROTEINS; GALACTOSIDASES; HALOTOLERANT; HIGH LEVEL EXPRESSION; HIGH SALT CONCENTRATION; INVERSE TRANSITION; NATURAL STRUCTURES; ONE-STEP PURIFICATION; OPTIMAL CONDITIONS; OPTIMIZED PROTOCOL; PROTEIN EXPRESSIONS; PROTEIN PURIFICATION; REVERSIBLE PHASE TRANSITION; SENSITIVE METHOD; TARGET PROTEINS; TOTAL PROTEIN;

CLONING; ENZYMES; ESCHERICHIA COLI; OPTIMIZATION; PROTEINS;

PURIFICATION;

ESCHERICHIA COLI;

EID: 84861337025     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2012.04.003     Document Type: Article

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