메뉴 건너뛰기




Volumn 415, Issue 2, 2011, Pages 200-202

An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

Author keywords

[No Author keywords available]

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; ELASTIN; GLYCOPROTEINS; PURIFICATION;

EID: 79958275550     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.04.034     Document Type: Article
Times cited : (19)

References (18)
  • 1
    • 0032739304 scopus 로고    scopus 로고
    • Purification of recombinant proteins by fusion with thermally-responsive polypeptides
    • DOI 10.1038/15100
    • D.E. Meyer, and A. Chilkoti Purification of recombinant proteins by fusion with thermally-responsive polypeptides Nat. Biotechnol. 17 1999 1112 1115 (Pubitemid 29533553)
    • (1999) Nature Biotechnology , vol.17 , Issue.11 , pp. 1112-1115
    • Meyer, D.E.1    Chilkoti, A.2
  • 2
    • 0026468684 scopus 로고
    • Free energy transduction in polypeptides and proteins based on inverse temperature transitions
    • D.W. Urry Free energy transduction in polypeptides and proteins based on inverse temperature transitions Prog. Biophys. Mol. Biol. 57 1992 23 57
    • (1992) Prog. Biophys. Mol. Biol. , vol.57 , pp. 23-57
    • Urry, D.W.1
  • 3
    • 0034878076 scopus 로고    scopus 로고
    • Protein purification by fusion with an environmentally responsive elastin-like polypeptide: Effect of polypeptide length on the purification of thioredoxin
    • DOI 10.1021/bp010049o
    • D.E. Meyer, K. Trabbic-Carlson, and A. Chilkoti Protein purification by fusion with an environmentally responsive elastin-like polypeptide: effect of polypeptide length on the purification of thioredoxin Biotechnol. Prog. 17 2001 720 728 (Pubitemid 32748063)
    • (2001) Biotechnology Progress , vol.17 , Issue.4 , pp. 720-728
    • Meyer, D.E.1    Trabbic-Carlson, K.2    Chilkoti, A.3
  • 4
    • 33845441925 scopus 로고    scopus 로고
    • Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling
    • DOI 10.1016/j.ab.2006.09.020, PII S0003269706007044
    • T. Christensen, K. Trabbic-Carlson, W. Liu, and A. Chilkoti Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling Anal. Biochem. 360 2007 166 168 (Pubitemid 44894337)
    • (2007) Analytical Biochemistry , vol.360 , Issue.1 , pp. 166-168
    • Christensen, T.1    Trabbic-Carlson, K.2    Liu, W.3    Chilkoti, A.4
  • 5
    • 33749581223 scopus 로고    scopus 로고
    • Simultaneous phase transition of ELP tagged molecules and free ELP: An efficient and reversible capture system
    • DOI 10.1021/bm060507n
    • X. Ge, and C.D. Filipe Simultaneous phase transition of ELP tagged molecules and free ELP: an efficient and reversible capture system Biomacromolecules 7 2006 2475 2478 (Pubitemid 44530483)
    • (2006) Biomacromolecules , vol.7 , Issue.9 , pp. 2475-2478
    • Ge, X.1    Filipe, C.D.M.2
  • 6
    • 33745026919 scopus 로고    scopus 로고
    • Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli
    • DOI 10.1021/bp0503742
    • D.C. Chow, M.R. Dreher, K. Trabbic-Carlson, and A. Chilkoti Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli Biotechnol. Prog. 22 2006 638 646 (Pubitemid 43877431)
    • (2006) Biotechnology Progress , vol.22 , Issue.3 , pp. 638-646
    • Chow, D.C.1    Dreher, M.R.2    Trabbic-Carlson, K.3    Chilkoti, A.4
  • 7
    • 9344234999 scopus 로고    scopus 로고
    • Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion
    • DOI 10.1110/ps.04931604
    • K. Trabbic-Carlson, L. Liu, B. Kim, and A. Chilkoti Expression and purification of recombinant proteins from Escherichia coli: comparison of an elastin-like polypeptide fusion with an oligohistidine fusion Protein Sci. 13 2004 3274 3284 (Pubitemid 39557797)
    • (2004) Protein Science , vol.13 , Issue.12 , pp. 3274-3284
    • Trabbic-Carlson, K.1    Liu, L.2    Kim, B.3    Chilkoti, A.4
  • 8
    • 0242680018 scopus 로고    scopus 로고
    • Thermally Triggered Purification and Immobilization of Elastin-OPH Fusions
    • DOI 10.1002/bit.10446
    • M. Shimazu, A. Mulchandani, and W. Chen Thermally triggered purification and immobilization of elastin-OPH fusions Biotechnol. Bioeng. 81 2003 74 79 (Pubitemid 38181640)
    • (2003) Biotechnology and Bioengineering , vol.81 , Issue.1 , pp. 74-79
    • Shimazu, M.1    Mulchandani, A.2    Chen, W.3
  • 9
    • 33748747042 scopus 로고    scopus 로고
    • Functional expression of a biologically active fragment of soluble gp130 as an ELP-fusion protein in transgenic plants: Purification via inverse transition cycling
    • DOI 10.1042/BJ20060544
    • M. Lin, S. Rose-John, J. Grotzinger, U. Conrad, and J. Scheller Functional expression of a biologically active fragment of soluble gp130 as an ELP-fusion protein in transgenic plants: purification via inverse transition cycling Biochem. J. 398 2006 577 583 (Pubitemid 44453403)
    • (2006) Biochemical Journal , vol.398 , Issue.3 , pp. 577-583
    • Lin, M.1    Rose-John, S.2    Grotzinger, J.3    Conrad, U.4    Scheller, J.5
  • 10
    • 36949012514 scopus 로고    scopus 로고
    • Heterologous expression and optimized one-step separation of levansucrase via elastin-like polypeptides tagging system
    • H.J. Kang, J.H. Kim, W.J. Chang, E.S. Kim, and Y.M. Koo Heterologous expression and optimized one-step separation of levansucrase via elastin-like polypeptides tagging system J. Microbiol. Biotechnol. 17 2007 1751 1757
    • (2007) J. Microbiol. Biotechnol. , vol.17 , pp. 1751-1757
    • Kang, H.J.1    Kim, J.H.2    Chang, W.J.3    Kim, E.S.4    Koo, Y.M.5
  • 11
    • 0842334816 scopus 로고    scopus 로고
    • Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: A role for surface hydrophobicity?
    • DOI 10.1093/protein/gzh006
    • K. Trabbic-Carlson, D.E. Meyer, L. Liu, R. Piervincenzi, N. Nath, T. LaBean, and A. Chilkoti Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity? Protein Eng. Des. Sel. 17 2004 57 66 (Pubitemid 38173169)
    • (2004) Protein Engineering, Design and Selection , vol.17 , Issue.1 , pp. 57-66
    • Trabbic-Carlson, K.1    Meyer, D.E.2    Liu, L.3    Piervincenzi, R.4    Nath, N.5    LaBean, T.6    Chilkoti, A.7
  • 12
    • 24044491571 scopus 로고    scopus 로고
    • Simple bioseparations using self-cleaving elastin-like polypeptide tags
    • DOI 10.1038/nmeth787
    • M.R. Banki, L. Feng, and D.W. Wood Simple bioseparations using self-cleaving elastin-like polypeptide tags Nat. Methods 2 2005 659 661 (Pubitemid 41223091)
    • (2005) Nature Methods , vol.2 , Issue.9 , pp. 659-661
    • Banki, M.R.1    Feng, L.2    Wood, D.W.3
  • 14
  • 15
    • 0033638223 scopus 로고    scopus 로고
    • Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast
    • E. Mossessova, and C.D. Lima Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast Mol. Cell 5 2000 865 876
    • (2000) Mol. Cell , vol.5 , pp. 865-876
    • Mossessova, E.1    Lima, C.D.2
  • 17
    • 0027109250 scopus 로고
    • Efficient expression and purification of a protease from the common cold virus, human rhinovirus type 14
    • DOI 10.1016/0022-0248(92)90253-F
    • L.E.-C. Leong, P.A. Walker, and A.G. Porter Efficient expression and purification of a protease from the common cold virus, human rhinovirus type 14 J. Cryst. Growth 122 1992 246 252 (Pubitemid 23577812)
    • (1992) Journal of Crystal Growth , vol.122 , Issue.1-4 , pp. 246-252
    • Leong, L.E.-C.1    Walker, P.A.2    Porter, A.G.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.