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Angewandte Chemie - International Edition

Volumn 52, Issue 13, 2013, Pages 3703-3708

Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides

(5) Bellucci, Joseph J a Amiram, Miriam a Bhattacharyya, Jayanta a McCafferty, Dewey b Chilkoti, Ashutosh a

a Duke University (United States)

b DUKE UNIVERSITY SCHOOL OF MEDICINE (United States)

Author keywords

anticancer agents; bioorganic chemistry; biotechnology; protein expression; Sortase A

Indexed keywords

ANTI-CANCER AGENTS; BIOACTIVE PROTEINS; BIOORGANIC CHEMISTRY; ELASTIN-LIKE POLYPEPTIDES; PROTEIN EXPRESSIONS; SITE-SPECIFIC MODIFICATIONS; SMALL MOLECULES; SORTASE A;

BIOCHEMISTRY; BIOTECHNOLOGY; CHROMATOGRAPHY; PURIFICATION;

RECOMBINANT PROTEINS;

AMINOACYLTRANSFERASE; ANTINEOPLASTIC AGENT; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; ELASTIN; HYBRID PROTEIN; SORTASE A; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND;

ANIMAL; ARTICLE; CELL SURVIVAL; CHEMISTRY; DRUG EFFECT; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; MOUSE; SITE DIRECTED MUTAGENESIS; STAPHYLOCOCCUS AUREUS; TUMOR CELL LINE;

AMINOACYLTRANSFERASES; ANIMALS; ANTINEOPLASTIC AGENTS; BACTERIAL PROTEINS; CELL LINE, TUMOR; CELL SURVIVAL; CYSTEINE ENDOPEPTIDASES; ELASTIN; ESCHERICHIA COLI; HUMANS; MICE; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS AUREUS; TNF-RELATED APOPTOSIS-INDUCING LIGAND; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84875332178 PISSN: 14337851 EISSN: 15213773 Source Type: Journal
DOI: 10.1002/anie.201208292 Document Type: Article

Times cited : (51)

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