Detection of fecal bacteria and source tracking identifiers in environmental waters using rRNA-based RT-qPCR and rDNA-based qPCR assays

Environmental Science and Technology

Volumn 47, Issue 23, 2013, Pages 13611-13620

  Pitkänen, Tarja ^a,b   Ryu, Hodon ^a   Elk, Michael ^a   Hokajärvi, Anna Maria ^b   Siponen, Sallamaari ^b   Vepsäläinen, Asko ^b   Räsänen, Pia ^b   Santo Domingo, Jorge W ^a  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^b NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL POPULATION; CAMPYLOBACTER SPP; DETECTION FREQUENCY; DETECTION SENSITIVITY; ENTEROCOCCUS SPP; ENVIRONMENTAL WATER; INTESTINAL ENTEROCOCCI; MICROBIAL WATER QUALITY;

ASSAYS; EFFLUENTS; ESCHERICHIA COLI; POLYMERASE CHAIN REACTION; RISK ASSESSMENT; SEWAGE; SURFACE WATERS; WATER QUALITY; WATER TREATMENT;

RNA;

RIBOSOME DNA; RIBOSOME RNA; SURFACE WATER; PRIMER DNA; RNA 16S; SEWAGE;

BACTERIUM; BIOASSAY; DNA; EFFLUENT; FREQUENCY ANALYSIS; GENE EXPRESSION; HEALTH RISK; IDENTIFICATION METHOD; POLYMERASE CHAIN REACTION; POPULATION STRUCTURE; RNA; WATER QUALITY;

ARTICLE; BACTERIUM DETECTION; BACTEROIDALES; CAMPYLOBACTER; CONTROLLED STUDY; DNA TEMPLATE; ENTEROCOCCUS; ESCHERICHIA COLI; FECES MICROFLORA; FLUOROMETER; GENE DOSAGE; GENE SEQUENCE; GENE TARGETING; HEAT TOLERANCE; MARKER GENE; PUBLIC HEALTH; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RISK ASSESSMENT; RNA EXTRACTION; SOURCE TRACKING IDENTIFIER; WATER CONTAMINATION; WATER QUALITY; WATER SAMPLING; WATER SUPPLY; BACTERIUM; BACTEROIDETES; ENVIRONMENTAL MONITORING; EVALUATION STUDY; FECES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; NUCLEOTIDE SEQUENCE; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; RNA GENE; SEWAGE; STATISTICS AND NUMERICAL DATA;

BACTERIA; BACTEROIDETES; BASE SEQUENCE; CAMPYLOBACTER; DNA PRIMERS; DNA, RIBOSOMAL; ENTEROCOCCUS; ENVIRONMENTAL MONITORING; ESCHERICHIA COLI; FECES; GENES, RRNA; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEWAGE; WATER MICROBIOLOGY;

EID: 84889784163     PISSN: 0013936X     EISSN: 15205851     Source Type: Journal    
DOI: 10.1021/es403489b     Document Type: Article

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