Development of quantitative PCR assays targeting the 16s rRNA genes of enterococcus spp. and their application to the identification of enterococcus species in environmental samples

Applied and Environmental Microbiology

Volumn 79, Issue 1, 2013, Pages 196-204

  Ryu, Hodon ^a   Henson, Michael ^a   Elk, Michael ^a   Toledo Hernandez, Carlos ^a   Griffith, John ^b   Blackwood, Denene ^c   Noble, Rachel ^c   Gourmelon, Michèle ^d   Glassmeyer, Susan ^e   Santo Domingo, Jorge W ^a  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^b SOUTHERN CALIFORNIA COASTAL WATER RESEARCH PROJECT   (United States)

^c The University of North Carolina at Chapel Hill   (United States)

^d IFREMER   (France)

^e U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA; 16S RRNA GENE; 16S RRNA GENE SEQUENCE; AMPLIFICATION RATE; ENTEROCOCCUS CASSELIFLAVUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS SPP; ENVIRONMENTAL SAMPLE; ENVIRONMENTAL WATER SAMPLES; FECAL SAMPLES; NON-TARGETED; POSITIVE SIGNALS; QUANTITATIVE PCR; RELATIVE ABUNDANCE; WATER SAMPLES;

BACILLI; GENES; POLYMERASE CHAIN REACTION; RNA; SIGNAL DETECTION;

ASSAYS;

BACTERIAL DNA; BACTERIAL RNA; PRIMER DNA; RIBOSOME DNA; RNA 16S;

BACTERIUM; BIOASSAY; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RELATIVE ABUNDANCE; SAMPLING; SPATIAL DISTRIBUTION; SPECIES DIVERSITY;

ARTICLE; CHEMISTRY; CLASSIFICATION; DNA SEQUENCE; ENTEROCOCCUS; EVALUATION; GENETIC VARIABILITY; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION;

BACTERIOLOGICAL TECHNIQUES; DNA PRIMERS; DNA, BACTERIAL; DNA, RIBOSOMAL; ENTEROCOCCUS; ENVIRONMENTAL MICROBIOLOGY; GENETIC VARIATION; MOLECULAR SEQUENCE DATA; REAL-TIME POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

ENTEROCOCCUS; ENTEROCOCCUS CASSELIFLAVUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM;

EID: 84871860476     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02802-12     Document Type: Article

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