Development of a sensitive rRNA-targeted reverse transcription-quantitative polymerase chain reaction for detection of Vibrio cholerae/mimicus, V. parahaemolyticus/alginolyticus and Campylobacter jejuni/coli

Microbiology and Immunology

Volumn 56, Issue 1, 2012, Pages 10-20

  Kurakawa, Takashi ^a   Kubota, Hiroyuki ^a,b   Tsuji, Hirokazu ^a   Matsuda, Kazunori ^a   Asahara, Takashi ^a   Takahashi, Takuya ^a   Ramamurthy, Thandavarayan ^c   Hamabata, Takashi ^d   Takahashi, Eizo ^e   Miyoshi, Shin Ichi ^e   Okamoto, Keinosuke ^e   Mukhopadhyay, Asish K ^c   Takeda, Yoshifumi ^f   Nomoto, Koji ^a  

^a YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

^b Yakult Honsha European Research Center for Microbiology   (Belgium)

^c NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES   (India)

^d NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE   (Japan)

^e OKAYAMA UNIVERSITY   (Japan)

^f JICA Building ID Hospital Campus   (India)

Author keywords

Campylobacter jejuni; RT qPCR; Vibrio cholerae; Vibrio parahaemolyticus

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; BACTERIAL RNA; RIBOSOME RNA;

ACCURACY; ARTICLE; BACTERIAL COUNT; BACTERIUM CULTURE; CAMPYLOBACTER JEJUNI; CONTROLLED STUDY; CULTURE TECHNIQUE; FECES ANALYSIS; FLUORESCENCE IN SITU HYBRIDIZATION; GENE TARGETING; HUMAN; IN VITRO CULTURE; INTERMETHOD COMPARISON; LIMIT OF DETECTION; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA STABILITY; STAINING; STORAGE TEMPERATURE; SUSPENSION; VIBRIO CHOLERAE; VIBRIO PARAHAEMOLYTICUS; VIRUS DETECTION;

BACTERIAL LOAD; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; DNA PRIMERS; FECES; GENES, RRNA; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; VIBRIO CHOLERAE; VIBRIO MIMICUS; VIBRIO PARAHAEMOLYTICUS;

BACTERIA (MICROORGANISMS); CAMPYLOBACTER; CAMPYLOBACTER JEJUNI; VIBRIO; VIBRIO CHOLERAE; VIBRIO PARAHAEMOLYTICUS;

EID: 84863137882     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2011.00405.x     Document Type: Article

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