Efficacy of bacterial ribosomal RNA-targeted reverse transcription-quantitative PCR for detecting neonatal sepsis: A case control study

BMC Pediatrics

Volumn 10, Issue , 2010, Pages

  Fujimori, Makoto ^a   Hisata, Ken ^a   Nagata, Satoru ^a,b   Matsunaga, Nobuaki ^a   Komatsu, Mitsutaka ^a   Shoji, Hiromichi ^a   Sato, Hiroaki ^a   Yamashiro, Yuichiro ^b   Asahara, Takashi ^c   Nomoto, Koji ^c   Shimizu, Toshiaki ^a  

^a JUNTENDO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b JUNTENDO UNIVERSITY   (Japan)

^c YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

C REACTIVE PROTEIN; BACTERIAL RNA; RIBOSOME RNA;

ABDOMINAL DISTENSION; APNEA; ARTERY PUNCTURE; ARTICLE; BACILLUS CEREUS; BACTERIAL RIBOSOMAL RNA TARGETED REVERSE TRANSCRIPTION QUANTITATIVE POLYMERASE CHAIN REACTION; BACTEROIDES FRAGILIS; BIFIDOBACTERIUM; BLOOD CULTURE; BLOOD SAMPLING; CASE CONTROL STUDY; CLINICAL ARTICLE; CLINICAL ASSESSMENT; CLOSTRIDIUM; CLOSTRIDIUM COCCOIDES; CLOSTRIDIUM LEPTUM; CLOSTRIDIUM PERFRINGENS; CYANOSIS; DIAGNOSTIC ACCURACY; DYSPNEA; ENTEROBACTERIACEAE; ENTEROCOCCUS; FEEDING DISORDER; FEVER; GENE AMPLIFICATION; HUMAN; HYPERGLYCEMIA; IRRITABILITY; MICROBIAL IDENTIFICATION; NEWBORN; NEWBORN SEPSIS; NUCLEIC ACID ANALYSIS; PATHOGENESIS; POLYMERASE CHAIN REACTION; PREVOTELLA; PSEUDOMONAS; RESPIRATORY DISTRESS; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS; STREPTOCOCCUS AGALACTIAE; TEMPERATURE MEASUREMENT; VOMITING; BACTERIAL INFECTION; BACTERIUM; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEPSIS;

BACTERIA; BACTERIAL INFECTIONS; BACTERIOLOGICAL TECHNIQUES; CASE-CONTROL STUDIES; HUMANS; INFANT, NEWBORN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL; SENSITIVITY AND SPECIFICITY; SEPSIS;

EID: 77955000563     PISSN: None     EISSN: 14712431     Source Type: Journal    
DOI: 10.1186/1471-2431-10-53     Document Type: Article

References (26)

1. Jordan JA, Durso MB, Butchko AR, Jones JG, Brozanski BS. Evaluating the near-term infant for early onset sepsis: progress and challenges to consider with 16S rDNA polymerase chain reaction testing. J Mol Diagn 2006, 8(3):357-363. 10.2353/jmoldx.2006.050138, 1867603, 16825509.
2. Cotten CM, Taylor S, Stoll B, Goldberg RN, Hansen NI, Sanchez PJ, Ambalavanan N, Benjamin DK. Prolonged duration of initial empirical antibiotic treatment is associated with increased rates of necrotizing enterocolitis and death for extremely low birth weight infants. Pediatrics 2009, 123(1):58-66. 10.1542/peds.2007-3423, 2760222, 19117861.
3. Del Vecchio A, Laforgia N, Capasso M, Iolascon A, Latini G. The role of molecular genetics in the pathogenesis and diagnosis of neonatal sepsis. Clin Perinatol 2004, 31(1):53-67. 10.1016/j.clp.2004.03.012, 15183656.
4. Jordan JA, Durso MB. Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis. J Mol Diagn 2005, 7(5):575-581. 1867550, 16258155.
5. Kaufman D, Fairchild KD. Clinical microbiology of bacterial and fungal sepsis in very-low-birth-weight infants. Clin Microbiol Rev 2004, 17(3):638-680. table of contents, 10.1128/CMR.17.3.638-680.2004, 452555, 15258097.
6. Ng PC, Lam HS. Diagnostic markers for neonatal sepsis. Curr Opin Pediatr 2006, 18(2):125-131. 10.1097/01.mop.0000193293.87022.4c, 16601490.
7. Malik A, Hui CP, Pennie RA, Kirpalani H. Beyond the complete blood cell count and C-reactive protein: a systematic review of modern diagnostic tests for neonatal sepsis. Arch Pediatr Adolesc Med 2003, 157(6):511-516. 10.1001/archpedi.157.6.511, 12796229.
8. Chan KY, Lam HS, Cheung HM, Chan AK, Li K, Fok TF, Ng PC. Rapid identification and differentiation of Gram-negative and Gram-positive bacterial bloodstream infections by quantitative polymerase chain reaction in preterm infants. Crit Care Med 2009, 37(8):2441-2447. 10.1097/CCM.0b013e3181a554de, 19531943.
9. Lam HS, Ng PC. Biochemical markers of neonatal sepsis. Pathology 2008, 40(2):141-148. 10.1080/00313020701813735, 18203036.
10. Frayha HH, Kalloghlian A. Gram-specific quantitative polymerase chain reaction for diagnosis of neonatal sepsis: implications for clinical practice. Crit Care Med 2009, 37(8):2487-2488. 10.1097/CCM.0b013e3181af1bd1, 19609126.
11. Jordan JA, Durso MB. Comparison of 16S rRNA gene PCR and BACTEC 9240 for detection of neonatal bacteremia. J Clin Microbiol 2000, 38(7):2574-2578. 86972, 10878046.
12. Laforgia N, Coppola B, Carbone R, Grassi A, Mautone A, Iolascon A. Rapid detection of neonatal sepsis using polymerase chain reaction. Acta Paediatr 1997, 86(10):1097-1099. 10.1111/j.1651-2227.1997.tb14815.x, 9350892.
13. Shang S, Chen G, Wu Y, Du L, Zhao Z. Rapid diagnosis of bacterial sepsis with PCR amplification and microarray hybridization in 16S rRNA gene. Pediatr Res 2005, 58(1):143-148. 10.1203/01.PDR.0000169580.64191.8B, 15985688.
14. Woo PC, Lau SK, Teng JL, Tse H, Yuen KY. Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin Microbiol Infect 2008, 14(10):908-934. 10.1111/j.1469-0691.2008.02070.x, 18828852.
15. Ohlin A, Backman A, Bjorkqvist M, Molling P, Jurstrand M, Schollin J. Real-time PCR of the 16S-rRNA gene in the diagnosis of neonatal bacteraemia. Acta Paediatr 2008, 97(10):1376-1380. 10.1111/j.1651-2227.2008.00924.x, 18624992.
16. Reier-Nilsen T, Farstad T, Nakstad B, Lauvrak V, Steinbakk M. Comparison of broad range 16S rDNA PCR and conventional blood culture for diagnosis of sepsis in the newborn: a case control study. BMC Pediatr 2009, 9:5. 10.1186/1471-2431-9-5, 2635358, 19152691.
17. Wu YD, Chen LH, Wu XJ, Shang SQ, Lou JT, Du LZ, Zhao ZY. Gram stain-specific-probe-based real-time PCR for diagnosis and discrimination of bacterial neonatal sepsis. J Clin Microbiol 2008, 46(8):2613-2619. 10.1128/JCM.02237-07, 2519463, 18550744.
18. Matsuda K, Tsuji H, Asahara T, Kado Y, Nomoto K. Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription-PCR. Appl Environ Microbiol 2007, 73(1):32-39. 10.1128/AEM.01224-06, 1797142, 17071791.
19. Kellogg JA, Manzella JP, Bankert DA. Frequency of low-level bacteremia in children from birth to fifteen years of age. J Clin Microbiol 2000, 38(6):2181-2185. 86758, 10834973.
20. Sakaguchi S, Saito M, Tsuji H, Asahara T, Takata O, Fujimura J, Nagata S, Nomoto K, Shimizu T. Bacterial Ribosomal RNA-Targeted Reverse Transcription PCR Used to Identify Pathogens Responsible for Fever with Neutropenia. J Clin Microbiol 2010, 48(5):1624-1628. 10.1128/JCM.01724-09, 20351213.
21. Wada M, Lkhagvadorj E, Bian L, Wang C, Chiba Y, Nagata S, Shimizu T, Yamashiro Y, Asahara T, Nomoto K. Quantitative reverse transcription-PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus. J Appl Microbiol 2010, 108(3):779-788. 10.1111/j.1365-2672.2009.04476.x, 19702857.
22. Hansen BM, Leser TD, Hendriksen NB. Polymerase chain reaction assay for the detection of Bacillus cereus group cells. FEMS Microbiol Lett 2001, 202(2):209-213. 10.1111/j.1574-6968.2001.tb10805.x, 11520616.
23. Tsujih H, Matsuda K, Asahara T, Kiwaki M, Nomoto K. Quantitative analysis of microbes with rRNA as target. 2006, In., vol. PCT/JP2006/301467. Japan;.
24. Matsuda K, Tsuji H, Asahara T, Matsumoto K, Takada T, Nomoto K. Establishment of an analytical system for the human fecal microbiota, based on reverse transcription-quantitative PCR targeting of multicopy rRNA molecules. Appl Environ Microbiol 2009, 75(7):1961-1969. 10.1128/AEM.01843-08, 2663197, 19201979.
25. Neal PR, Kleiman MB, Reynolds JK, Allen SD, Lemons JA, Yu PL. Volume of blood submitted for culture from neonates. J Clin Microbiol 1986, 24(3):353-356. 268912, 3760131.
26. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM. New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 2004, 4(12):751-760. 10.1016/S1473-3099(04)01205-8, 15567125.