Knockdown of AMP-activated protein kinase alpha 1 and alpha 2 catalytic subunits

Journal of RNAi and Gene Silencing

Volumn 8, Issue 1, 2012, Pages 470-478

  Tangeman, Larissa ^a   Wyatt, Christopher N ^a   Brown, Thomas L ^a  

^a WRIGHT STATE UNIVERSITY   (United States)

Author keywords

Ampk; Ampk 1; Ampk 2; Prkaa; shrna; Sirna

Indexed keywords

ACETYL COENZYME A CARBOXYLASE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE ALPHA 1; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE ALPHA 2; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVE SITE; FIBROBLAST; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PROTEIN PHOSPHORYLATION; RNA INTERFERENCE;

EID: 84887602089     PISSN: 17470854     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Aguilar V, Alliouachene S, Sotiropoulos A et al, 2007. S6 kinase depletion suppresses muscle growth adaptations to nutrient availability by activating AMP kinase. Cell Metab, 5, 476-487.
2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal Biochem. 72, 248-254.
3. Brummelkamp TR, Bernards R and Agami R. 2002a. Stable suppression of tumorigenicity by virus-mediated RNA interference, Cancer Cell, 2, 243-247.
4. Brummelkamp TR, Bernards R and Agami R. 2002b. A system for stable expression of short interfering RNAs in mammalian cells, Science, 296, 550-553.
5. Carling D. 2005. AMP-activated protein kinase: balancingg the scales. Biochimie, 87, 87-91.
6. Carthew RW and Sontheimer EJ. 2009. Origins and Mechanisms of miRNAs and siRNAs. Cell, 136, 642-55.
7. Dale S, Wilson WA, Edelman AM et al. 1995. Similar substrate recognition motifs for mammalian AMP-activated protein kinase, higher plant HMG-CoA reductase kinase-A, yeast SNF1, and mammalian calmodulin-dependent protein kinase I. FEBS Lett, 361,191-195.
8. Elbashir SM, Harbouth J, Lendeckel W et al, 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, 411, 494-498.
9. Emerling BM, Weinberg F, Snyder C et al. 2009. Hypoxic activation of AMPK is dependent on mitochondrial ROS but independent of an increase in AMP/ATP ratio. Free Radic Biol Med, 46, 1386-1391.
10. Evans AM, Mustard KJ, Wyatt CN et al. 2005. Does AMP-activated protein kinase couple inhibition of mitochondrial oxidative phosphorylation by hypoxia to calcium signaling in O2-sensing cells? J Biol Chem, 280, 41504-41511.
11. Fire A, Xu S, Montgomery MK et al. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391, 806-811.
12. Geiduscheck EP and Tocchini-Valentini GP. 1988. Transcription by RNA polymerase III, Ann Rev Biochem, 57, 873-914.
13. Guigas B, Taleux N, Foretz M et al. 2007. AMP-activated protein kinase-independent inhibition of hepatic mitochondrial oxidative phosphorylation by AICA riboside. Biochem J, 404, 499-507.
14. Gultice AD, Kulkarni-Datar K and Brown TL. 2009. Hypoxia-inducible factor 1alpha (HIF1A) mediates distinct steps of rat trophoblast differentiation in gradient oxygen. Biol Reprod, 80, 184-193.
15. Ha J, Daniel S, Broyles SS et al. 1994. Critical phosphorylation sites for acetyl-CoA carboxylase activity. J Biol Chem, 269, 22162-22168.
16. Harborth J, Elbashir SM, Vandenburgh K et al. 2003. Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing. Antisense Nucleic Acid Drug Dev, 13, 83-105.
17. Hardie DG. 2011. AMP-activated protein kinase: a cellular energy sensor with a key role in metabolic disorders and in cancer. Biochem Soc Trans, 39, 1-13.
18. Hardie DG. 2007. AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy. Nat Rev Mol Cell Biol, 8, 774-785.
19. Hawley SA, Ross FA, Chevtzoff C et al. 2010. Use of cells expressing gamma subunit variants to identify diverse mechanisms of AMPK activation. Cell Metab, 11, 554-565.
20. Hitz C, Steuber-Buchberger P, Delic S et al. 2009. Generation of shRNA transgenic mice. Methods Mol Biol, 530, 101-129.
21. Herold MJ, van Den Brandt J, Seibler J et al. 2008. Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats. Proc Natl Acad Sci USA. 105, 18507-18512.
22. Horman S, Browne G, Krause U et al. 2002. Activation of AMP-activated protein kinase leads to the phosphorylation of elongation factor 2 and an inhibition of protein synthesis. Curr Biol, 12, 1419-1423.
23. Jørgensen SB, Wojtaszewski JF, Viollet B et al. 2005. Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle. FASEB J, 19, 1146-1148.
24. Kim D and Rossi J. 2008. RNAi mechanisms and applications. Biotechniques, 44, 613-616.
25. Neurath KM, Keough MP, Mikkelsen T et al. 2006. AMP-dependent protein kinase alpha 2 isoform promotes hypoxia-induced VEGF expression in human glioblastoma. Glia, 53, 733-743.
26. Park SH, Gammon SR, Knippers JD et al. 2002. Phosphorylation-activity relationships of AMPK and acetyl-CoA carboxylase in muscle. J Appl Physiol, 92, 2475-2482.
27. Schopman NC, Liu YP, Konstantinova P et al. 2010. Optimization of shRNA inhibitors by variation of the terminal loop sequence. Antiviral Res, 86, 204-211.
28. Semizarov D, Frost L, Sarthy A et al. 2003 Specificity of short interfering RNA determined through gene expression signatures. Proc Natl Acad Sci USA, 100, 6347-6352.
29. Steinberg GR and Kemp BE. 2009. AMPK in Health and Disease. Physiol Rev, 89, 1025-1078.
30. Viollet B, Andreelli F, Jørgensen SB et al. 2003. Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem Soc Trans, 31, 216-219.
31. Viollet B, Athea Y, Mounier R et al. 2009. AMPK: Lessons from transgenic and knockout animals. Front Biosci, 14, 19-44.
32. Vucicevic L, Misirkic M, Janjetovic K et al. 2009. AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C. Biochem Pharmacol, 77, 1684-1693.
33. Xing Y, Musi N, Fujii N et al. 2003. Glucose metabolism and energy homeostasis in mouse hearts overexpressing dominant negative alphα2 subunit of AMP-activated protein kinase. J Biol Chem, 278, 28372-28377.
34. Zhou J, Huang W, Tao R et al. 2009. Inactivation of AMPK alters gene expression and promotes growth of prostate cancer cells. Oncogene, 28,1993-2002.