Malachite green mediates homodimerization of antibody VL domains to form a fluorescent ternary complex with singular symmetric interfaces

Journal of Molecular Biology

Volumn 425, Issue 22, 2013, Pages 4595-4613

  Szent Gyorgyi, Chris ^a   Stanfield, Robyn L ^b   Andreko, Susan ^a   Dempsey, Alison ^a   Ahmed, Mushtaq ^a   Capek, Sarah ^c   Waggoner, Alan S ^a,c   Wilson, Ian A ^b   Bruchez, Marcel P ^a,c  

^a CARNEGIE MELLON UNIVERSITY   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

cooperative binding; directed evolution; fluorogen activating protein; quaternary structure; yeast surface display

Indexed keywords

ANTIBODY; DYE; FLUOROGEN; IMMUNOGLOBULIN LIGHT CHAIN; LIGAND; MALACHITE GREEN; TERNARY COMPLEX FACTOR; UNCLASSIFIED DRUG;

ANTIBODY AFFINITY; ARTICLE; BINDING AFFINITY; BIOSENSOR; DIMERIZATION; ENTHALPY; FLUORESCENCE; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN ASSEMBLY;

EID: 84886718229     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2013.08.014     Document Type: Article

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